Rhesus Recombinant CD1B & B2M protein, Hetero Dimer , His Tag (V2LY-0526-LY9666)

Name: Rhesus Recombinant CD1B & B2M protein, Hetero Dimer , His Tag
SKU: V2LY-0526-LY9666
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Rhesus

Tag

His Tag

Protein Construction

This product is Rhesus Recombinant CD1B & B2M protein, Hetero Dimer , His Tag consist of Amino Acid: 1-303, 1-119 and predicts a molecular mass of 44.6 kDa.

Molecule Mass

44.6 kDa

Polymeric

Hetero Dimer

Sequence

Amino Acid: 1-303, 1-119

Species

Rhesus

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>95% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tris, NaCl, Glutathione, EDTA, DTT, PMSF, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

CD1b Molecule

Function

Antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells.

Biological Process

Adaptive immune response Source: UniProtKB-KW
Antigen processing and presentation, endogenous lipid antigen via MHC class Ib Source: GO_Central
Antigen processing and presentation, exogenous lipid antigen via MHC class Ib Source: GO_Central
Positive regulation of T cell mediated cytotoxicity Source: GO_Central
Regulation of immune response Source: Reactome

Cellular Location

Lysosome membrane; Endosome membrane; Cell membrane. Subject to intracellular trafficking between the cell membrane, endosomes and lysosomes.

Topology

Extracellular: 18-303
Helical: 304-324
Cytoplasmic: 325-333

Ween, M. P., White, J. B., Tran, H. B., Mukaro, V., Jones, C., Macowan, M., ... & Hodge, S. J. (2021). The role of oxidised self-lipids and alveolar macrophage CD1b expression in COPD. Scientific reports, 11(1), 1-14.

Guo, J., Jin, H., Xi, Y., Guo, J., Jin, Y., & Jiang, D. (2020). The miR-582/CD1B axis is involved in regulation of dendritic cells and is associated with clinical outcomes in advanced lung adenocarcinoma. BioMed research international, 2020.

Reijneveld, J. F., Ocampo, T. A., Shahine, A., Gully, B. S., Vantourout, P., Hayday, A. C., ... & Van Rhijn, I. (2020). Human γδ T cells recognize CD1b by two distinct mechanisms. Proceedings of the National Academy of Sciences, 117(37), 22944-22952.

Camacho, F., Moreno, E., Garcia-Alles, L., Santiago, G. C., Gilleron, M., Vasquez, A., ... & Acosta, A. (2020). A direct role for the CD1b endogenous spacer in the recognition of a Mycobacterium tuberculosis antigen by T-cell receptors. Frontiers in immunology, 11, 2538.

Shahine, A., Reinink, P., Reijneveld, J. F., Gras, S., Holzheimer, M., Cheng, T. Y., ... & Van Rhijn, I. (2019). A T-cell receptor escape channel allows broad T-cell response to CD1b and membrane phospholipids. Nature communications, 10(1), 1-12.

Lee, C. H., Chen, L. C., Yu, C. C., Lin, W. H., Lin, V. C., Huang, C. Y., ... & Bao, B. Y. (2019). Prognostic value of CD1B in localised prostate cancer. International journal of environmental research and public health, 16(23), 4723.

Shahine, A. (2018). The intricacies of self-lipid antigen presentation by CD1b. Molecular immunology, 104, 27-36.

Chancellor, A., Tocheva, A. S., Cave-Ayland, C., Tezera, L., White, A., Juma’a, R., ... & Mansour, S. (2017). CD1b-restricted GEM T cell responses are modulated by Mycobacterium tuberculosis mycolic acid meromycolate chains. Proceedings of the National Academy of Sciences, 114(51), E10956-E10964.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant CD1B & B2M protein, Hetero Dimer , His Tag (CAT#: V2LY-0526-LY2715)

Human Recombinant CD1B protein, hFc Tag (CAT#: V2LY-0526-LY2716)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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