GH1 Antibodies

Structure of GH1

The molecular weight of the globulin subunit encoded by the GH1 gene is approximately 16.0 kDa, and its precise value varies slightly with species differences. This protein is composed of 146 amino acids and achieves oxygen transport function through the precise coordination of histidine residues and heme cofactors. The GH1 sequences of different mammals show a high degree of conservation, and the amino acid substitution at key sites directly affects the oxygen affinity of hemoglobin.

Fig. 1 The Cartoon Structure of a GH1 Family β-Glucosidase.¹

Key structural properties of GH1:

• Form a typical α -helical folded conformation
• Hydrophobic pockets precisely fit heme excipients
• Histidine residues at E7 and F8 coordinate to regulate oxygenation balance
• The β-chain tetrameric interface maintains the stability of the quaternary structure of hemoglobin

Functions of GH1

The β -globin encoded by the GH1 gene is the core component of hemoglobin tetramer, and its main physiological functions include:

Unlike the monomer high affinity of myoglobin, the hemoglobin formed by GH1 shows a typical S-shaped oxygen dissociation curve, achieving efficient oxygen transport and release through subunit synergistic effects.

Applications of GH1 and GH1 Antibody in Literature

1. Chagas, Rafael S., et al. "Mechanism of imidazole inhibition of a GH1 β‐glucosidase." FEBS Open Bio 13.5 (2023): 912-925. https://doi.org/10.1002/2211-5463.13595

Research has found that imidazole can bind to the conserved active center of GH1 β -glucosidase through a partially competitive inhibition mechanism, reducing substrate affinity and thereby affecting enzyme activity. This phenomenon may be widespread in the GH1 family and is of great significance for the study of recombinase.

2. Plaza-Vinuesa, Laura, et al. "Dual 6Pβ-galactosidase/6Pβ-glucosidase GH1 family for lactose metabolism in the probiotic bacterium Lactiplantibacillus plantarum WCFS1." Journal of Agricultural and Food Chemistry 71.28 (2023): 10693-10700. https://doi.org/10.1021/acs.jafc.3c01158

Research has found that the GH1 family protein Lp_3525 in Lactobacillus plantarum has a unique 6Pβ -galactosidase activity. This enzyme belongs to a novel bifunctive GH1 protein and can effectively hydrolyze lactose under intestinal conditions, providing new insights into alleviating lactose dyspepsia.

3. Khusnutdinova, Anna N., et al. "Moderately thermostable GH1 β-glucosidases from hyperacidophilic archaeon Cuniculiplasma divulgatum S5." FEMS microbiology ecology 100.9 (2024): fiae114. https://doi.org/10.1093/femsec/fiae114

Two GH1 family β -glucosidases, CIB12 and CIB13, were identified in hyperacidophilic archaea. They have a typical TIM-barrel structure, with an optimal temperature of 50° C. They mainly function as cellobiose hydrolase, which helps archaea utilize cellulose carbon sources in peptide environments and enhances environmental adaptability.

4. Park, Dongryeol, et al. "Bacillus velezensis GH1-13 enhances drought tolerance in rice by reducing the accumulation of reactive oxygen species." Frontiers in Plant Science 15 (2024): 1432494. https://doi.org/10.3389/fpls.2024.1432494

Research has found that the GH1-13 strain enhances the expression of antioxidant genes in rice by activating the jasmonic acid signaling pathway, effectively eliminating reactive oxygen species under drought stress, thereby significantly improving the drought tolerance of rice.

5. Huang X, Chen H, et al. "The clinical and genetic aspects of six individuals with GH1 variants and isolated growth hormone deficiency type II." Frontiers in Endocrinology 15 (2024): 1363050. https://doi.org/10.3389/fendo.2024.1363050

In this study, GH1 gene variations, including four de novo mutations, were identified in six Chinese families with type II isolated growth hormone deficiency. All patients presented with severe growth hormone deficiency and short stature. rhGH replacement therapy could effectively improve their height.

Creative Biolabs: GH1 Antibodies for Research

Creative Biolabs specializes in the production of high-quality GH1 antibodies for research and industrial applications. Our portfolio includes monoclonal antibodies tailored for ELISA, Flow Cytometry, Western blot, immunohistochemistry, and other diagnostic methodologies.

• Custom GH1 Antibody Development: Tailor-made solutions to meet specific research requirements.
• Bulk Production: Large-scale antibody manufacturing for industry partners.
• Technical Support: Expert consultation for protocol optimization and troubleshooting.
• Aliquoting Services: Conveniently sized aliquots for long-term storage and consistent experimental outcomes.

For more details on our GH1 antibodies, custom preparations, or technical support, contact us at email.