GSTP1 Antibodies

Structure of GSTP1

GSTP1 is a relatively small protein with a molecular weight of approximately 23.2 kDa. This molecular weight may vary slightly among different species, mainly due to variations in the amino acid sequence encoded by the gene.

This protein is composed of 209 amino acids and forms a compact spherical homodimer through its primary structure folding. The three-dimensional structure of GSTP1 contains a hydrophilic glutathione binding site (G-site) and a hydrophobic substrate binding pocket (H-site). This structure enables it to catalyze various electrophilic poison-glutathione binding reactions. The key tyrosine residue in its active center is crucial for promoting the deprotonation of glutathione and initiating nucleophilic reactions. The secondary structure of the protein is mainly composed of α-helices and β-sheets, which jointly maintain the stable conformation required for its catalytic function.

Fig. 1 Modeled structure of parts of GSTP1.¹

Key structural properties of GSTP1:

• Typical thioredoxin folding structure
• Hydrophobic/hydrophilic compatible substrate binding pocket
• Active site tyrosine residues mediated catalytic reaction
• Homologous dimer interface through hydrogen bonding network stable conformation

Functions of GSTP1

The main function of the GSTP1 protein is to catalyze the binding of various electrophilic toxins with reduced glutathione (GSH), thereby enabling the detoxification of cells. Additionally, it is deeply involved in regulating the cellular redox balance, signal transduction, and drug metabolism, among other physiological and pathological processes.

Unlike many enzymatic reactions, the catalytic action of GSTP1 on GSH follows the "ping-pong" mechanism. That is, GSH first binds and undergoes deprotonation before the enzyme-glutathione complex combines with the hydrophobic substrate to complete the reaction. This enables it to efficiently handle a wide variety of toxic substances.

Applications of GSTP1 and GSTP1 Antibody in Literature

1. Sun, Yandi, et al. "A GSTP1-mediated lactic acid signaling promotes tumorigenesis through the PPP oxidative branch." Cell Death & Disease 14.7 (2023): 463. https://doi.org/10.1038/s41419-023-05998-4

Studies have shown that the tumor metabolite lactic acid inhibits the phosphorylation of G6PD by binding to GSTP1, enhances the generation of NADPH in the pentose phosphate pathway, and thereby maintains redox homeostasis and promotes breast cancer growth. This mechanism is particularly significant in certain breast cancer subtypes.

2. Lei, Xiao, et al. "GSTP1 as a novel target in radiation induced lung injury." Journal of translational medicine 19.1 (2021): 297. https://doi.org/10.1186/s12967-021-02978-0

Studies have shown that GSTP1 is the main antioxidant enzyme in lung tissue and is associated with the risk of radiation-induced lung injury. It participates in the disease process by regulating oxidative stress and cell apoptosis. Specific gene polymorphisms can affect susceptibility.

3. Santric, Veljko, et al. "GSTP1 rs1138272 polymorphism affects prostate cancer risk." Medicina 56.3 (2020): 128. https://doi.org/10.3390/medicina56030128

The study found that two specific mutations (rs1695 and rs1138272) of the GSTP1 gene were significantly associated with the risk of prostate cancer. Especially when both mutations occurred simultaneously, the risk of the disease increased by 5.46 times. The co-occurrence of multiple GST gene mutations would further synergistically increase the risk.

4. Fang, Cheng, et al. "Aberrant GSTP1 promoter methylation is associated with increased risk and advanced stage of breast cancer: a meta-analysis of 19 case-control studies." BMC cancer 15.1 (2015): 920. https://doi.org/10.1186/s12885-015-1926-1

The meta-analysis confirmed that abnormal methylation of the GSTP1 gene promoter is significantly associated with the risk of breast cancer (OR = 7.85), and it is more common in advanced patients. This change exists in different ethnic groups and can serve as a potential biomarker for breast cancer diagnosis.

5. Miao, Li-Feng, Xiang-Hua Ye, and Xiao-Feng He. "Individual and combined effects of GSTM1, GSTT1, and GSTP1 polymorphisms on breast cancer risk: a meta-analysis and re-analysis of systematic meta-analyses." PloS one 15.3 (2020): e0216147. https://doi.org/10.1371/journal.pone.0216147

This study, through a meta-analysis of 101 articles, indicates that the evidence linking the polymorphisms of the GSTM1, GSTT1 and GSTP1 genes (including both individual and combined effects) to an increased risk of breast cancer is limited. In high-quality studies, the reliability of most positive results is relatively low.

Creative Biolabs: GSTP1 Antibodies for Research

Creative Biolabs specializes in the production of high-quality GSTP1 antibodies for research and industrial applications. Our portfolio includes monoclonal and polyclonal antibodies tailored for ELISA, Flow Cytometry, Western blot, immunohistochemistry, and other diagnostic methodologies.

• Custom GSTP1 Antibody Development: Tailor-made solutions to meet specific research requirements.
• Bulk Production: Large-scale antibody manufacturing for industry partners.
• Technical Support: Expert consultation for protocol optimization and troubleshooting.
• Aliquoting Services: Conveniently sized aliquots for long-term storage and consistent experimental outcomes.

For more details on our GSTP1 antibodies, custom preparations, or technical support, contact us at info@creative-biolabs.com.