Mouse Anti-ALDH3A1 Recombinant Antibody (B8) (CBMAB-A2280-YC)

Name: Mouse Anti-ALDH3A1 Recombinant Antibody (B8)
SKU: CBMAB-A2280-YC
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-ALDH3A1 Recombinant Antibody (B8) in WB

Western blot analysis was used to compare the expression of different ALDH isozymes GC1401 (lane 1), GC1415 (lane 2) and GC1436 (lane 3) GC cell lines. GAPDH served as loading control. ...View More

Mytar, B., Stec, M., Szatanek, R., Węglarczyk, K., Szewczyk, K., Szczepanik, A., ... & Baj‑Krzyworzeka, M. (2018). Characterization of human gastric adenocarcinoma cell lines established from peritoneal ascites. Oncology Letters, 15(4), 4849-4858.

Mouse Anti-ALDH3A1 Recombinant Antibody (B8) in WB

Immunoblot analysis of ALDH isoforms in NSCLC cell lines. IMR-90 and normal lung airway cells were used as non-cancer controls, whereas β-actin was used as a loading control. ...View More

Kang, J. H., Lee, S. H., Hong, D., Lee, J. S., Ahn, H. S., Ahn, J. H., ... & Kim, S. Y. (2016). Aldehyde dehydrogenase is used by cancer cells for energy metabolism. Experimental & molecular medicine, 48(11), e272-e272.

Mouse Anti-ALDH3A1 Recombinant Antibody (B8) in IHC

A case of HCC with high ALDH3A1 expression, with scarce clusters of positive cells in the adjacent tumour-free liver tissue (a). Both cytoplasmic and nuclear ALDH3A1 immunoreactivity was observed (b). ...View More

Calderaro, J., Nault, J. C., Bioulac-Sage, P., Laurent, A., Blanc, J. F., Decaens, T., & Zucman-Rossi, J. (2014). ALDH3A1 is overexpressed in a subset of hepatocellular carcinoma characterised by activation of the Wnt/β-catenin pathway. Virchows Archiv, 464, 53-60.

Mouse Anti-ALDH3A1 Recombinant Antibody (B8) in IHC

Striking centrilobular ALDH3A1 expression in tumour-free liver tissue at low (a) and high magnification (b). ...View More

Mouse Anti-ALDH3A1 Recombinant Antibody (B8) in WB

Cell lysates were collected and western blot analysis was carried out for DU145 and DU145 spheres to determine ALDH3A1 expression (top). RNA was isolated from both cell lines and examined for ALDH3A1 mRNA expression using real-time PCR (bottom). β-actin was used as an internal control. ALDH3A1 mRNA in DU145 spheres is shown as a fold change to DU145 cells (mean±s.d.). *P<0.05 by a Mann-Whitney test. ...View More

Yan, J., De Melo, J., Cutz, J. C., Aziz, T., & Tang, D. (2014). Aldehyde dehydrogenase 3A1 associates with prostate tumorigenesis. British journal of cancer, 110(10), 2593-2603.

Mouse Anti-ALDH3A1 Recombinant Antibody (B8) in IF

Keratocytes in the stromal part of the hemicornea were observed after 4',6-diamidino-2-phenylindole (DAPI) staining (A). Expression of specific markers of the corneal stroma was analyzed: keratan sulfate (KS), essential for the maintenance of the correct orientation of collagen fibrils (B); CD-34, a specific marker of keratocytes expressed both in vivo and in vitro (C); ALDH3A1, a marker of quiescent keratocytes in vivo (D); VSX1, found in keratocytes undergoing fibroblastic transformation (E); α-SMA, a muscle protein of F-actin stress fibers, typically found in myofibroblasts (F). Scale bar=50 μm. ...View More

Barbaro, V., Ferrari, S., Fasolo, A., Ponzin, D., & Di Iorio, E. (2009). Reconstruction of a human hemicornea through natural scaffolds compatible with the growth of corneal epithelial stem cells and stromal keratocytes. Molecular Vision, 15, 2084.

Mouse Anti-ALDH3A1 Recombinant Antibody (B8) in ICC

Immunocytological phenotyping of primary muscle cells in culture. Human cells were extracted from TFL muscles of healthy donors and grown in culture in proliferation medium (two passages, A) or in proliferation (two passages) and then in differentiation medium (4 days, B). Cells were fixed using PFA and permeabilized using saponin; and then incubated with antibodies directed against ALDH isoenzymes, and desmin; and then incubated with labelled secondary antibodies. Nuclei were stained using DAPI (blue). In proliferation (A), the number of cells expressing isoenzymes (red, first column) generally exceeded the number of cells expressing desmin (green, second column) (ALDH3A2, 3B2, 7A1, 8A1, and 9A1, arrows) except for 1A1 where some desmin+ cells were negative for the isoenzyme. ALDH3A2 stained strongly some nuclei. Following differentiation (B), myotubes were generally labelled with a stronger intensity than mononucleated cells (ALDH3A1, 3B1, and 9A1). The intensities of ALDH1A1 and 2 labelling were decreased as compared with cells in proliferation. Several nuclei strongly expressed ALDH3A2, suggesting a translocation from cytoplasm to nucleus. Original magnifications: ×40. ...View More

Etienne, J., Joanne, P., Catelain, C., Riveron, S., Bayer, A. C., Lafable, J., ... & Vilquin, J. T. (2020). Aldehyde dehydrogenases contribute to skeletal muscle homeostasis in healthy, aging, and Duchenne muscular dystrophy patients. Journal of cachexia, sarcopenia and muscle, 11(4), 1047-1069.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

Application

ELISA, ICC, IF, IHC, WB

Immunogen

Amino acids 31-106 mapping near the N-terminus of ALDH3A1 of human origin.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
ELISA	1:30-1:3,000
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
IHC	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin.

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Aldehyde Dehydrogenase 3 Family Member A1

Introduction

Aldehyde dehydrogenases oxidize various aldehydes to the corresponding acids. They are involved in the detoxification of alcohol-derived acetaldehyde and in the metabolism of corticosteroids, biogenic amines, neurotransmitters, and lipid peroxidation. The

Entrez Gene ID

218

UniProt ID

P30838

Alternative Names

Aldehyde Dehydrogenase 3 Family Member A1; Aldehyde Dehydrogenase, Dimeric NADP-Preferring; EC 1.2.1.5; ALDHIII; ALDH3; Aldehyde Dehydrogenase 3 Family, Member A1; Aldehyde Dehydrogenase Family 3 Member A1;

Function

ALDHs play a major role in the detoxification of alcohol-derived acetaldehyde (Probable). They are involved in the metabolism of corticosteroids, biogenic amines, neurotransmitters, and lipid peroxidation (Probable). Oxidizes medium and long chain aldehydes into non-toxic fatty acids (PubMed:1737758). Preferentially oxidizes aromatic aldehyde substrates (PubMed:1737758). Comprises about 50 percent of corneal epithelial soluble proteins (By similarity). May play a role in preventing corneal damage caused by ultraviolet light (By similarity).

Biological Process

Aging Source: Ensembl
Cellular aldehyde metabolic process Source: UniProtKB
Positive regulation of cell population proliferation Source: Ensembl
Response to cAMP Source: Ensembl
Response to drug Source: Ensembl
Response to glucocorticoid Source: Ensembl
Response to hypoxia Source: Ensembl
Response to nutrient Source: Ensembl
Xenobiotic metabolic process Source: Reactome

Cellular Location

Cytoplasm

Suwala, A. K., Koch, K., Rios, D. H., Aretz, P., Uhlmann, C., Ogorek, I., ... & Maciaczyk, J. (2018). Inhibition of Wnt/beta-catenin signaling downregulates expression of aldehyde dehydrogenase isoform 3A1 (ALDH3A1) to reduce resistance against temozolomide in glioblastoma in vitro. Oncotarget, 9(32), 22703.

Saiki, J. P., Cao, H., Van Wassenhove, L. D., Viswanathan, V., Bloomstein, J., Nambiar, D. K., ... & Mochly-Rosen, D. (2018). Aldehyde dehydrogenase 3A1 activation prevents radiation-induced xerostomia by protecting salivary stem cells from toxic aldehydes. Proceedings of the National Academy of Sciences, 115(24), 6279-6284.

Duong, H. Q., You, K. S., Oh, S., Kwak, S. J., & Seong, Y. S. (2017). Silencing of NRF2 reduces the expression of ALDH1A1 and ALDH3A1 and sensitizes to 5-FU in pancreatic cancer cells. Antioxidants, 6(3), 52.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Antibody Pairs

ALDH3A1 Matched Antibody Pair (063) (CAT#: APMAB-063LY)

ALDH3A1 Matched Antibody Pair (064) (CAT#: APMAB-064LY)

Related Products

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Rabbit Anti-ALDH3A1 Recombinant Antibody (BA0332) (CAT#: CBMAB-0058CQ)

ALDH3A1 Matched Antibody Pair (063) (CAT#: APMAB-063LY)

ALDH3A1 Matched Antibody Pair (064) (CAT#: APMAB-064LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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