Dog ALDH3A1 ELISA Kit (V2LY-0626-LY831)

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Tested Data
Datasheet
SDS
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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity
0.011 ng/mL
Detection Range
0.02-3.5 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Dog
Assay Type
Sandwich
Reactivity
Dog
Assay Time
1.5 h
Molecule Mass
50.7 kDa
Components
  • Pre-coated ELISA plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
Aldehyde Dehydrogenase 3 Family Member A1
Function
ALDHs play a major role in the detoxification of alcohol-derived acetaldehyde (Probable). They are involved in the metabolism of corticosteroids, biogenic amines, neurotransmitters, and lipid peroxidation (Probable). Oxidizes medium and long chain aldehydes into non-toxic fatty acids (PubMed:1737758). Preferentially oxidizes aromatic aldehyde substrates (PubMed:1737758). Comprises about 50 percent of corneal epithelial soluble proteins (By similarity). May play a role in preventing corneal damage caused by ultraviolet light (By similarity).
Biological Process
Aging Source: Ensembl
Cellular aldehyde metabolic process Source: UniProtKB
Positive regulation of cell population proliferation Source: Ensembl
Response to cAMP Source: Ensembl
Response to drug Source: Ensembl
Response to glucocorticoid Source: Ensembl
Response to hypoxia Source: Ensembl
Response to nutrient Source: Ensembl
Xenobiotic metabolic process Source: Reactome
Cellular Location
Cytoplasm

Suwala, A. K., Koch, K., Rios, D. H., Aretz, P., Uhlmann, C., Ogorek, I., ... & Maciaczyk, J. (2018). Inhibition of Wnt/beta-catenin signaling downregulates expression of aldehyde dehydrogenase isoform 3A1 (ALDH3A1) to reduce resistance against temozolomide in glioblastoma in vitro. Oncotarget, 9(32), 22703.

Saiki, J. P., Cao, H., Van Wassenhove, L. D., Viswanathan, V., Bloomstein, J., Nambiar, D. K., ... & Mochly-Rosen, D. (2018). Aldehyde dehydrogenase 3A1 activation prevents radiation-induced xerostomia by protecting salivary stem cells from toxic aldehydes. Proceedings of the National Academy of Sciences, 115(24), 6279-6284.

Duong, H. Q., You, K. S., Oh, S., Kwak, S. J., & Seong, Y. S. (2017). Silencing of NRF2 reduces the expression of ALDH1A1 and ALDH3A1 and sensitizes to 5-FU in pancreatic cancer cells. Antioxidants, 6(3), 52.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant ALDH3A1 protein, His Tag (CAT#: V2LY-0526-LY1999)

ALDH3A1 Matched Antibody Pair (063) (CAT#: APMAB-063LY)

ALDH3A1 Matched Antibody Pair (064) (CAT#: APMAB-064LY)

Antibody Pairs

ALDH3A1 Matched Antibody Pair (063) (CAT#: APMAB-063LY)

ALDH3A1 Matched Antibody Pair (064) (CAT#: APMAB-064LY)

Related Products

ALDH3A1 Matched Antibody Pair (063) (CAT#: APMAB-063LY)

Rabbit Anti-ALDH3A1 Recombinant Antibody (BA0332) (CAT#: CBMAB-0058CQ)

Mouse Anti-ALDH3A1 Recombinant Antibody (B8) (CAT#: CBMAB-A2280-YC)

Human Recombinant ALDH3A1 protein, His Tag (CAT#: V2LY-0526-LY1999)

Mouse Anti-ALDH3A1 Recombinant Antibody (G2) (CAT#: CBMAB-A2282-YC)

ALDH3A1 Matched Antibody Pair (064) (CAT#: APMAB-064LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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