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Mouse Anti-ALDH3A1 Recombinant Antibody (G2) (CBMAB-A2282-YC)

Provided herein is a Mouse monoclonal antibody against Human Aldehyde Dehydrogenase 3 Family Member A1. The antibody can be used for immunoassay techniques, such as ELISA, IP.
See all ALDH3A1 antibodies
Published Data
Mouse Anti-ALDH3A1 Recombinant Antibody (G2) in Immunoblot
Immunoblot analysis was carried out using ALDH1A1, 1A3, and 3A1 specific antibodies in a panel of immortalized PCa cell lines (PC-3, LNCaP, and DU145) and using GAPDH as a control. ...View More
Ibrahim, A. I., Batlle, E., Sneha, S., Jiménez, R., Pequerul, R., Parés, X., ... & Pors, K. (2022). Expansion of the 4-(Diethylamino) benzaldehyde scaffold to explore the impact on aldehyde dehydrogenase activity and antiproliferative activity in prostate cancer. Journal of Medicinal Chemistry, 65(5), 3833-3848.
Mouse Anti-ALDH3A1 Recombinant Antibody (G2) in WB
Western blot showed ALDH3A1 was knocked down by ALDH3A1 lentiviral shRNA. ...View More
Li, W., Wilson, G. C., Bachmann, M., Wang, J., Mattarei, A., Paradisi, C., ... & Patel, S. H. (2022). Inhibition of a mitochondrial potassium channel in combination with gemcitabine and abraxane drastically reduces pancreatic ductal adenocarcinoma in an immunocompetent orthotopic murine model. Cancers, 14(11), 2618.
Mouse Anti-ALDH3A1 Recombinant Antibody (G2) in IHC
Immunohistochemical detection of macrophages and analysis of keratocyte phenotype in the healed cornea at 30 hours post debridement. (a) Immunohistochemistry detects F4/80-labeled macrophages, ALDH3A1 (keratocyte marker), and aSMA (myofibroblast marker). F4/80-labeled macrophages were more frequently observed beneath the regenerated epithelium in the KO mouse (B) compared with the WT cornea (A). The cells in the posterior stroma were labeled for ALDH3A1, while fibroblastic cells in the anterior stroma were not labeled in the WT cornea (C). In the KO cornea, the majority of the cells in the stroma were negative for ALDH3A1 (D). A few a-smooth muscle actin-positive myofibroblasts were detected in the WT stroma (E). Almost all the stromal cells were labeled with anti-aSMA antibody and thus were myofibroblasts in the KO cornea (F). Scale bar: 50 lm. Real-time PCR indicated no difference in the mRNA expression level of ALDH3A1 (b) and aSMA (c). ...View More
Shirai, K., Okada, Y., Cheon, D. J., Miyajima, M., Behringer, R. R., Yamanaka, O., & Saika, S. (2014). Effects of the loss of conjunctival Muc16 on corneal epithelium and stroma in mice. Investigative ophthalmology & visual science, 55(6), 3626-3637.
Mouse Anti-ALDH3A1 Recombinant Antibody (G2) in WB
Effect of EPQ on the expression of ALDH-related proteins in acetaldehyde-treated PC12 cells. Cells were treated with DMSO (0.5%, v/v) or EPQ (20 μM) in the presence and absence of acetaldehyde (500 μM) for 24 h. The oxidative stress-related protein levels of ALDH1A1, ALDH2A1, and ALDH3A1 were determined by the western blots. GAPDH was a loading control. All values are expressed as mean ± SEM (n = 3; ** p < 0.01 compared to the DMSO control group and ## p < 0.01 compared to the group treated only with ACT). EPQ, ent-peniciherqueinone; DMSO, dimethyl sulfoxide; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; ALDH, aldehyde dehydrogenase. ...View More
Oh, T., Kwon, M., Yu, J. S., Jang, M., Kim, G. H., Kim, K. H., ... & Ahn, J. S. (2020). Ent-peniciherqueinone suppresses acetaldehyde-induced cytotoxicity and oxidative stress by inducing ALDH and suppressing MAPK signaling. Pharmaceutics, 12(12), 1229.
Mouse Anti-ALDH3A1 Recombinant Antibody (G2) in WB
Western blotting analysis of ALDH3A1, GPX2, GLRX, and PDK4 expression in A549, WT, and DEL clones in normoxia or hypoxia. HSP60 was used as a loading control. ...View More
Moreno Leon, L., Gautier, M., Allan, R., Ilié, M., Nottet, N., Pons, N., ... & Rezzonico, R. (2019). The nuclear hypoxia-regulated NLUCAT1 long non-coding RNA contributes to an aggressive phenotype in lung adenocarcinoma through regulation of oxidative stress. Oncogene, 38(46), 7146-7165.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
G2
Antibody Isotype
IgG1, κ
Application
ELISA, ICC, IF, IHC, WB

Basic Information

Immunogen
Amino acids 31-106 mapping near the N-terminus of ALDH3A1 of mouse origin.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
ELISA1:30-1:3,000
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
IHC1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin.
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Aldehyde Dehydrogenase 3 Family Member A1
Introduction
Aldehyde dehydrogenases oxidize various aldehydes to the corresponding acids. They are involved in the detoxification of alcohol-derived acetaldehyde and in the metabolism of corticosteroids, biogenic amines, neurotransmitters, and lipid peroxidation. The
Entrez Gene ID
218
UniProt ID
P30838
Alternative Names
Aldehyde Dehydrogenase 3 Family Member A1; Aldehyde Dehydrogenase, Dimeric NADP-Preferring; EC 1.2.1.5; ALDHIII; ALDH3; Aldehyde Dehydrogenase 3 Family, Member A1; Aldehyde Dehydrogenase Family 3 Member A1;
Function
ALDHs play a major role in the detoxification of alcohol-derived acetaldehyde (Probable). They are involved in the metabolism of corticosteroids, biogenic amines, neurotransmitters, and lipid peroxidation (Probable). Oxidizes medium and long chain aldehydes into non-toxic fatty acids (PubMed:1737758). Preferentially oxidizes aromatic aldehyde substrates (PubMed:1737758). Comprises about 50 percent of corneal epithelial soluble proteins (By similarity). May play a role in preventing corneal damage caused by ultraviolet light (By similarity).
Biological Process
Aging Source: Ensembl
Cellular aldehyde metabolic process Source: UniProtKB
Positive regulation of cell population proliferation Source: Ensembl
Response to cAMP Source: Ensembl
Response to drug Source: Ensembl
Response to glucocorticoid Source: Ensembl
Response to hypoxia Source: Ensembl
Response to nutrient Source: Ensembl
Xenobiotic metabolic process Source: Reactome
Cellular Location
Cytoplasm
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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