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Mouse Anti-ARHGDIB Recombinant Antibody (97A1015) (CBMAB-D0026-YC)


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Summary

Host Animal
Mouse
Specificity
Human, Mouse
Clone
97A1015
Antibody Isotype
IgG2b, κ
Application
FC, WB, IHC, IP

Basic Information

Immunogen
A synthetic peptide corresponding to the region of the Fas-induced cleavage site of human D4-GDI/RhoGDI2 was used as the immunogen.
Specificity
Human, Mouse
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
FC0.5-1 µg/10^6 cells
WB2 µg/ml
IP1-2 µg/ml
IHC-P1:10-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS
Preservative
0.05% sodium azide
Concentration
1 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Rho GDP Dissociation Inhibitor Beta
Introduction
Members of the Rho protein family and other Ras-related small GTP-binding proteins are involved in diverse cellular events, including cell signaling, proliferation, cytoskeletal organization, and secretion. The GTP-binding proteins are active only in the GTP-bound state. At least 3 classes of proteins tightly regulate cycling between the GTP-bound and GDP-bound states: GTPase-activating proteins (GAPs), guanine nucleotide-releasing factors (GRFs), and GDP-dissociation inhibitors (GDIs). The GDIs, including ARHGDIB, decrease the rate of GDP dissociation from Ras-like GTPases.
Entrez Gene ID
397
UniProt ID
P52566
Alternative Names
Rho GDP Dissociation Inhibitor Beta; Rho GDP Dissociation Inhibitor (GDI) Beta; Rho-GDI Beta; Rho GDI 2; RAP1GN1; Ly-GDI; GDIA2;
Function
Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them (PubMed:8356058, PubMed:7512369).
Regulates reorganization of the actin cytoskeleton mediated by Rho family members (PubMed:8262133).
Biological Process
Cellular response to redox state Source: Ensembl
Multicellular organism development Source: ProtInc
Negative regulation of cell adhesion Source: ProtInc
Negative regulation of trophoblast cell migration Source: CACAO
Positive regulation of GTPase activity Source: GOC
Regulation of actin cytoskeleton reorganization Source: UniProtKB
Regulation of Rho protein signal transduction Source: UniProtKB
Regulation of small GTPase mediated signal transduction Source: Reactome
Rho protein signal transduction Source: GO_Central
Cellular Location
Cytosol
More Infomation

Betjes, M. G., Sablik, K. A., Litjens, N. H., Otten, H. G., & de Weerd, A. E. (2021). ARHGDIB and AT1R autoantibodies are differentially related to the development and presence of chronic antibody-m

Wang, X., Bi, X., Huang, X., Wang, B., Guo, Q., & Wu, Z. (2020). Systematic investigation of biomarker-like role of ARHGDIB in breast cancer. Cancer Biomarkers, 28(1), 101-110.

Senev, A., Otten, H. G., Kamburova, E. G., Callemeyn, J., Lerut, E., Van Sandt, V., ... & Naesens, M. (2020). Antibodies against ARHGDIB and ARHGDIB gene expression associate with kidney allograft outcome. Transplantation, 104(7), 1462-1471.

Kardol-Hoefnagel, T., van Logtestijn, S. A., & Otten, H. G. (2020). A review on the function and regulation of ARHGDIB/RhoGDI2 expression including the hypothetical role of ARHGDIB/RhoGDI2 autoantibodies in kidney transplantation. Transplantation direct, 6(5).

Kamburova, E. G., Gruijters, M. L., Kardol‐Hoefnagel, T., Wisse, B. W., Joosten, I., Allebes, W. A., ... & Otten, H. G. (2019). Antibodies against ARHGDIB are associated with long‐term kidney graft loss. American Journal of Transplantation, 19(12), 3335-3344.

Cao, L., Lafyatis, R., & Burkly, L. C. (2017). Increased dermal collagen bundle alignment in systemic sclerosis is associated with a cell migration signature and role of Arhgdib in directed fibroblast migration on aligned ECMs. PloS one, 12(6), e0180751.

von Klot, C. A., Dubrowinskaja, N., Peters, I., Hennenlotter, J., Merseburger, A. S., Stenzl, A., ... & Serth, J. (2017). Rho GDP dissociation inhibitor‑β in renal cell carcinoma. Oncology letters, 14(6), 8190-8196.

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For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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