Sign in or Register   Sign in or Register
  |  

Mouse Anti-ARHGDIB Recombinant Antibody (CBYC-A758) (CBMAB-A3514-YC)

Provided herein is a Mouse monoclonal antibody against Human Rho GDP Dissociation Inhibitor Beta. The antibody can be used for immunoassay techniques, such as FC, IHC, IP, WB.
See all ARHGDIB antibodies
Published Data
Mouse Anti-ARHGDIB Recombinant Antibody (CBYC-A758) CBMAB-A3514-YC in Immunoblot
Representative Immunoblot using antibodies anti-RhoGDI3, anti-RhoGDI2 and anti-GAPDH were used as loading control. Total lysates from hTERT-HPNE, BxPC3 and PANC-1 cell lines were analyzed. ...View More
de León-Bautista, M. P., Cardenas-Aguayo, M. D. C., Casique-Aguirre, D., Almaraz-Salinas, M., Parraguirre-Martinez, S., Olivo-Diaz, A., ... & Vargas, M. (2016). Immunological and functional characterization of RhoGDI3 and its molecular targets RhoG and RhoB in human pancreatic cancerous and normal cells. PLoS One, 11(11), e0166370.

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBYC-A758
Antibody Isotype
IgG3, κ
Application
WB, IP, IF, ELISA

Basic Information

Immunogen
Amino acids 45-74 within an internal region of Ly-GDI of human origin.
Specificity
Human
Antibody Isotype
IgG3, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Rho GDP Dissociation Inhibitor Beta
Introduction
Members of the Rho (or ARH) protein family and other Ras-related small GTP-binding proteins are involved in diverse cellular events, including cell signaling, proliferation, cytoskeletal organization, and secretion. The GTP-binding proteins are active onl
Entrez Gene ID
397
UniProt ID
P52566
Alternative Names
Rho GDP Dissociation Inhibitor Beta; Rho GDP Dissociation Inhibitor (GDI) Beta; Rho-GDI Beta; Rho GDI 2; RAP1GN1; Ly-GDI; GDIA2;
Function
Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them (PubMed:8356058, PubMed:7512369).
Regulates reorganization of the actin cytoskeleton mediated by Rho family members (PubMed:8262133).
Biological Process
Cellular response to redox state Source: Ensembl
Multicellular organism development Source: ProtInc
Negative regulation of cell adhesion Source: ProtInc
Negative regulation of trophoblast cell migration Source: CACAO
Positive regulation of GTPase activity Source: GOC
Regulation of actin cytoskeleton reorganization Source: UniProtKB
Regulation of Rho protein signal transduction Source: UniProtKB
Regulation of small GTPase mediated signal transduction Source: Reactome
Rho protein signal transduction Source: GO_Central
Cellular Location
Cytosol

Betjes, M. G., Sablik, K. A., Litjens, N. H., Otten, H. G., & de Weerd, A. E. (2021). ARHGDIB and AT1R autoantibodies are differentially related to the development and presence of chronic antibody-m

Wang, X., Bi, X., Huang, X., Wang, B., Guo, Q., & Wu, Z. (2020). Systematic investigation of biomarker-like role of ARHGDIB in breast cancer. Cancer Biomarkers, 28(1), 101-110.

Senev, A., Otten, H. G., Kamburova, E. G., Callemeyn, J., Lerut, E., Van Sandt, V., ... & Naesens, M. (2020). Antibodies against ARHGDIB and ARHGDIB gene expression associate with kidney allograft outcome. Transplantation, 104(7), 1462-1471.

Kardol-Hoefnagel, T., van Logtestijn, S. A., & Otten, H. G. (2020). A review on the function and regulation of ARHGDIB/RhoGDI2 expression including the hypothetical role of ARHGDIB/RhoGDI2 autoantibodies in kidney transplantation. Transplantation direct, 6(5).

Kamburova, E. G., Gruijters, M. L., Kardol‐Hoefnagel, T., Wisse, B. W., Joosten, I., Allebes, W. A., ... & Otten, H. G. (2019). Antibodies against ARHGDIB are associated with long‐term kidney graft loss. American Journal of Transplantation, 19(12), 3335-3344.

Cao, L., Lafyatis, R., & Burkly, L. C. (2017). Increased dermal collagen bundle alignment in systemic sclerosis is associated with a cell migration signature and role of Arhgdib in directed fibroblast migration on aligned ECMs. PloS one, 12(6), e0180751.

von Klot, C. A., Dubrowinskaja, N., Peters, I., Hennenlotter, J., Merseburger, A. S., Stenzl, A., ... & Serth, J. (2017). Rho GDP dissociation inhibitor‑β in renal cell carcinoma. Oncology letters, 14(6), 8190-8196.

Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Mouse Anti-ARHGDIB Recombinant Antibody (CBYC-A758)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Go to
Compare