Mouse Anti-ARHGDIB Recombinant Antibody (CBYC-A759) (CBMAB-A3515-YC)

Name: Mouse Anti-ARHGDIB Recombinant Antibody (CBYC-A759)
SKU: CBMAB-A3515-YC
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-ARHGDIB Recombinant Antibody (CBYC-A759) CBMAB-A3515-YC in IHC

Rho-GDIβ and XIAP proteins level was evaluated by using IHC staining in bladder cancer tissues in comparison to the paired normal bladder tissues (n=32). ...View More

Yu, Y., Jin, H., Xu, J., Gu, J., Li, X., Xie, Q., ... & Huang, C. (2018). XIAP overexpression promotes bladder cancer invasion in vitro and lung metastasis in vivo via enhancing nucleolin-mediated Rho-GDIβ mRNA stability. International journal of cancer, 142(10), 2040-2055.

Mouse Anti-ARHGDIB Recombinant Antibody (CBYC-A759) CBMAB-A3515-YC in WB

Representative Western blots of RhoGDI1 and RhoGDI2 expression in prostate cell lines. ...View More

Baker, M. J., Abba, M. C., Garcia-Mata, R., & Kazanietz, M. G. (2020). P-REX1-independent, calcium-dependent RAC1 hyperactivation in prostate cancer. Cancers, 12(2), 480.

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBYC-A759

Application

WB, IP, IF, ELISA, IHC-P

Immunogen

Amino acids 1-201 representing full length Ly-GDI of human origin.

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Rho GDP Dissociation Inhibitor Beta

Introduction

Members of the Rho (or ARH) protein family and other Ras-related small GTP-binding proteins are involved in diverse cellular events, including cell signaling, proliferation, cytoskeletal organization, and secretion. The GTP-binding proteins are active onl

Entrez Gene ID

397

UniProt ID

P52566

Alternative Names

Rho GDP Dissociation Inhibitor Beta; Rho GDP Dissociation Inhibitor (GDI) Beta; Rho-GDI Beta; Rho GDI 2; RAP1GN1; Ly-GDI; GDIA2;

Function

Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them (PubMed:8356058, PubMed:7512369).
Regulates reorganization of the actin cytoskeleton mediated by Rho family members (PubMed:8262133).

Biological Process

Cellular response to redox state Source: Ensembl
Multicellular organism development Source: ProtInc
Negative regulation of cell adhesion Source: ProtInc
Negative regulation of trophoblast cell migration Source: CACAO
Positive regulation of GTPase activity Source: GOC
Regulation of actin cytoskeleton reorganization Source: UniProtKB
Regulation of Rho protein signal transduction Source: UniProtKB
Regulation of small GTPase mediated signal transduction Source: Reactome
Rho protein signal transduction Source: GO_Central

Cellular Location

Cytosol

Betjes, M. G., Sablik, K. A., Litjens, N. H., Otten, H. G., & de Weerd, A. E. (2021). ARHGDIB and AT1R autoantibodies are differentially related to the development and presence of chronic antibody-m

Wang, X., Bi, X., Huang, X., Wang, B., Guo, Q., & Wu, Z. (2020). Systematic investigation of biomarker-like role of ARHGDIB in breast cancer. Cancer Biomarkers, 28(1), 101-110.

Senev, A., Otten, H. G., Kamburova, E. G., Callemeyn, J., Lerut, E., Van Sandt, V., ... & Naesens, M. (2020). Antibodies against ARHGDIB and ARHGDIB gene expression associate with kidney allograft outcome. Transplantation, 104(7), 1462-1471.

Kardol-Hoefnagel, T., van Logtestijn, S. A., & Otten, H. G. (2020). A review on the function and regulation of ARHGDIB/RhoGDI2 expression including the hypothetical role of ARHGDIB/RhoGDI2 autoantibodies in kidney transplantation. Transplantation direct, 6(5).

Kamburova, E. G., Gruijters, M. L., Kardol‐Hoefnagel, T., Wisse, B. W., Joosten, I., Allebes, W. A., ... & Otten, H. G. (2019). Antibodies against ARHGDIB are associated with long‐term kidney graft loss. American Journal of Transplantation, 19(12), 3335-3344.

Cao, L., Lafyatis, R., & Burkly, L. C. (2017). Increased dermal collagen bundle alignment in systemic sclerosis is associated with a cell migration signature and role of Arhgdib in directed fibroblast migration on aligned ECMs. PloS one, 12(6), e0180751.

von Klot, C. A., Dubrowinskaja, N., Peters, I., Hennenlotter, J., Merseburger, A. S., Stenzl, A., ... & Serth, J. (2017). Rho GDP dissociation inhibitor‑β in renal cell carcinoma. Oncology letters, 14(6), 8190-8196.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Antibody Pairs

ARHGDIB Matched Antibody Pair (102) (CAT#: APMAB-102LY)

ARHGDIB Matched Antibody Pair (103) (CAT#: APMAB-103LY)

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ARHGDIB Matched Antibody Pair (102) (CAT#: APMAB-102LY)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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