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Rabbit Anti-ASH2L Recombinant Antibody (10E6) (CBMAB-A3774-YC)

Provided herein is a Rabbit monoclonal antibody against Human ASH2 Like Histone Lysine Methyltransferase Complex Subunit. The antibody can be used for immunoassay techniques, such as IF, IP, WB.
See all ASH2L antibodies
Published Data
Rabbit Anti-ASH2L Recombinant Antibody (10E6) CBMAB-A3774-YC in ICC
Immunocytochemical detection of the proteins described in A in WT and mutant oocytes. At least 60 oocytes were analyzed in each group; representative images are shown. ...View More
Ma, P., Pan, H., Montgomery, R. L., Olson, E. N., & Schultz, R. M. (2012). Compensatory functions of histone deacetylase 1 (HDAC1) and HDAC2 regulate transcription and apoptosis during mouse oocyte development. Proceedings of the National Academy of Sciences, 109(8), E481-E489.
Rabbit Anti-ASH2L Recombinant Antibody (10E6) CBMAB-A3774-YC in WB
The reduced abundances of WRR5, Ash2, SET1 and p65 proteins in HepG2 and Huh-7 cells transfected with siMKL1. WRR5, Ash2, SET1 and p65 protein levels in HCC cells were detected by western blotting, with GAPDH as the internal standard. Full-length blots/gels are presented in Additional files 1C *P < 0.05; **P < 0.01 ...View More
Liu, Z., Sun, J., Li, C., Xu, L., & Liu, J. (2021). MKL1 regulates hepatocellular carcinoma cell proliferation, migration and apoptosis via the COMPASS complex and NF-κB signaling. BMC cancer, 21(1), 1-11.
Rabbit Anti-ASH2L Recombinant Antibody (10E6) CBMAB-A3774-YC in IP
PASK immunoprecipitated with V5 antibody from the nuclear extract of HEK 293T cells transfected with a hPASK expression plasmid with a V5 tag. PASK along with the core members of the H3K4 MLL2 methyltransferase complex, WDR5, ASH2 RBBP5 and the methyltransferase enzyme MLL2 are co-immunoprecipitated in the presence of V5 antibody. Nuclear extract from V5 vector transfected HEK 293T cells was used as control. About 10% of the nuclear extract was used as input. The results shown are a representative of three independent experiments. ...View More
Karakkat, J. V., Kaimala, S., Sreedharan, S. P., Jayaprakash, P., Adeghate, E. A., Ansari, S. A., ... & Starling Emerald, B. (2019). The metabolic sensor PASK is a histone 3 kinase that also regulates H3K4 methylation by associating with H3K4 MLL2 methyltransferase complex. Nucleic acids research, 47(19), 10086-10103.

Summary

Host Animal
Rabbit
Specificity
Human, Monkey, Mouse, Rat
Clone
10E6
Antibody Isotype
IgG
Application
IF, IP, WB

Basic Information

Immunogen
Synthetic peptide corresponding to residues surrounding Gly265 of human ASH2L protein.
Specificity
Human, Monkey, Mouse, Rat
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:2,000
IP1:100
IF(ICC)1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
10mM HEPES, pH 7.5, 0.15M NaCl, 0.1mg/mL BSA, 50% Glycerol
Preservative
0.02% sodium azide
Concentration
Batch dependent
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
ASH2 Like Histone Lysine Methyltransferase Complex Subunit
Introduction
Component of the Set1/Ash2 histone methyltransferase (HMT) complex, a complex that specifically methylates Lys-4 of histone H3, but not if the neighboring Lys-9 residue is already methylated. As part of the MLL1/MLL complex it is involved in methylation a
Entrez Gene ID
Human9070
Mouse23808
Rat290829
Monkey699382
UniProt ID
HumanQ9UBL3
MouseQ91X20
RatD3ZTV7
MonkeyA0A2K5TX06
Alternative Names
ASH2 Like Histone Lysine Methyltransferase Complex Subunit; ASH2-Like Protein; ASH2L1; Set1/Ash2 Histone Methyltransferase Complex Subunit ASH2; Ash2 (Absent, Small, Or Homeotic)-Like (Drosophila); Ash2 (Absent, Small, Or Homeotic)-Like; ASH2L2; ASH2; Bre
Function
Transcriptional regulator (PubMed:12670868).
Component or associated component of some histone methyltransferase complexes which regulates transcription through recruitment of those complexes to gene promoters (PubMed:19131338).
Component of the Set1/Ash2 histone methyltransferase (HMT) complex, a complex that specifically methylates 'Lys-4' of histone H3, but not if the neighboring 'Lys-9' residue is already methylated (PubMed:19556245).
As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3 (PubMed:19556245).
May play a role in hematopoiesis (PubMed:12670868).
In association with RBBP5 and WDR5, stimulates the histone methyltransferase activities of KMT2A, KMT2B, KMT2C, KMT2D, SETD1A and SETD1B (PubMed:21220120, PubMed:22266653).
Biological Process
Beta-catenin-TCF complex assembly Source: Reactome
Cellular response to DNA damage stimulus Source: MGI
Hemopoiesis Source: UniProtKB
Histone H3-K4 methylation Source: UniProtKB
Positive regulation of cell population proliferation Source: UniProtKB
Regulation of megakaryocyte differentiation Source: Reactome
Response to estrogen Source: UniProtKB
Cellular Location
Nucleus
PTM
Both monomethylated and dimethylated on arginine residues in the C-terminus. Arg-296 is the major site. Methylation is not required for nuclear localization, nor for MLL complex integrity or maintenance of global histone H3K4me3 levels.
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For research use only. Not intended for any clinical use.

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