Human Recombinant ASH2L protein, GST Tag (V2LY-0526-LY2185)

Name: Human Recombinant ASH2L protein, GST Tag
SKU: V2LY-0526-LY2185
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

E. coli

Protein Species

Human

Tag

GST Tag

Protein Construction

This product is Human Recombinant ASH2L protein, GST Tag consist of Amino Acid: Full Length and predicts a molecular mass of 94 kDa.

Molecule Mass

94 kDa

Sequence

Amino Acid: Full Length

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

Batch dependent.

Endotoxin

Please contact us for more information.

Format

Liquid

Buffer

Tris, NaCl, Glutathione, EDTA, DTT, PMSF, Glycerol

Preservative

None

Storage

Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

More Infomation

Target

Full Name

ASH2 Like Histone Lysine Methyltransferase Complex Subunit

Function

Transcriptional regulator (PubMed:12670868).
Component or associated component of some histone methyltransferase complexes which regulates transcription through recruitment of those complexes to gene promoters (PubMed:19131338).
Component of the Set1/Ash2 histone methyltransferase (HMT) complex, a complex that specifically methylates 'Lys-4' of histone H3, but not if the neighboring 'Lys-9' residue is already methylated (PubMed:19556245).
As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3 (PubMed:19556245).
May play a role in hematopoiesis (PubMed:12670868).
In association with RBBP5 and WDR5, stimulates the histone methyltransferase activities of KMT2A, KMT2B, KMT2C, KMT2D, SETD1A and SETD1B (PubMed:21220120, PubMed:22266653).

Biological Process

Beta-catenin-TCF complex assembly Source: Reactome
Cellular response to DNA damage stimulus Source: MGI
Hemopoiesis Source: UniProtKB
Histone H3-K4 methylation Source: UniProtKB
Positive regulation of cell population proliferation Source: UniProtKB
Regulation of megakaryocyte differentiation Source: Reactome
Response to estrogen Source: UniProtKB

Cellular Location

Nucleus

PTM

Both monomethylated and dimethylated on arginine residues in the C-terminus. Arg-296 is the major site. Methylation is not required for nuclear localization, nor for MLL complex integrity or maintenance of global histone H3K4me3 levels.

Lee, Y. T., Ayoub, A., Park, S. H., Sha, L., Xu, J., Mao, F., ... & Dou, Y. (2021). Mechanism for DPY30 and ASH2L intrinsically disordered regions to modulate the MLL/SET1 activity on chromatin. Nature communications, 12(1), 1-13.

Wu, Y. J., Li, L. X., Liu, L., Zhao, S. S., Qiu, H. R., & Wang, H. (2020). ASH2L-Promoted HOXC8 Gene Expression Plays a Role in Mixed Lineage Leukemia-Rearranged Acute Leukemia. OncoTargets and therapy, 13, 381.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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