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Mouse Anti-DEPTOR Recombinant Antibody (5B3) (CBMAB-A2189-LY)

The product is antibody recognizes DEPDC6. The antibody 5B3 immunoassay techniques such as: sELISA, ELISA.
See all DEPTOR antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
5B3
Antibody Isotype
IgG2a, κ
Application
sELISA, ELISA

Basic Information

Immunogen
DEPDC6 (AAH12040, 1 a.a. ~ 409 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG2a, κ
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
DEP Domain Containing MTOR Interacting Protein
Entrez Gene ID
64798
UniProt ID
Q8TB45
Alternative Names
DEP.6; DKFZp564B1778; FLJ12341; FLJ12428; FLJ13854
Function
Negative regulator of the mTORC1 and mTORC2 signaling pathways. Inhibits the kinase activity of both complexes.
Biological Process
Intracellular signal transduction Source: InterPro
Negative regulation of cell size Source: UniProtKB
Negative regulation of protein kinase activity Source: UniProtKB
Negative regulation of TOR signaling Source: UniProtKB
Regulation of extrinsic apoptotic signaling pathway Source: UniProtKB
PTM
Phosphorylated. Phosphorylation weakens interaction with MTOR within mTORC1 and mTORC2.
Ubiquitinated; ubiquitination leads to proteasomal degradation.

Wälchli, M., Berneiser, K., Mangia, F., Imseng, S., Craigie, L. M., Stuttfeld, E., ... & Maier, T. (2021). Regulation of human mTOR complexes by DEPTOR. Elife, 10, e70871.

Perez-Tejeiro, J. M., & Csukasi, F. (2021). DEPTOR in Skeletal Development and Diseases. Frontiers in Genetics, 12.

Heimhalt, M., Berndt, A., Wagstaff, J., Anandapadamanaban, M., Perisic, O., Maslen, S., ... & Williams, R. L. (2021). Bipartite binding and partial inhibition links DEPTOR and mTOR in a mutually antagonistic embrace. Elife, 10, e68799.

Chen, S., Jia, L., Zhang, S., Zheng, Y., & Zhou, Y. (2018). DEPTOR regulates osteogenic differentiation via inhibiting MEG3-mediated activation of BMP4 signaling and is involved in osteoporosis. Stem Cell Research & Therapy, 9(1), 1-14.

Varusai, T. M., & Nguyen, L. K. (2018). Dynamic modelling of the mTOR signalling network reveals complex emergent behaviours conferred by DEPTOR. Scientific reports, 8(1), 1-14.

Wang, Q., Zhou, Y., Rychahou, P., Harris, J. W., Zaytseva, Y. Y., Liu, J., ... & Evers, B. M. (2018). Deptor is a novel target of Wnt/β-catenin/c-Myc and contributes to colorectal cancer cell growth. Cancer research, 78(12), 3163-3175.

Chen, H., Shen, F., Sherban, A., Nocon, A., Li, Y., Wang, H., ... & Zang, M. (2018). DEP domain–containing mTOR–interacting protein suppresses lipogenesis and ameliorates hepatic steatosis and acute‐on‐chronic liver injury in alcoholic liver disease. Hepatology, 68(2), 496-514.

Caron, A., Briscoe, D. M., Richard, D., & Laplante, M. (2018). DEPTOR at the nexus of cancer, metabolism, and immunity. Physiological reviews, 98(3), 1765-1803.

Catena, V., & Fanciulli, M. (2017). Deptor: not only a mTOR inhibitor. Journal of Experimental & Clinical Cancer Research, 36(1), 1-9.

Caron, A., Mouchiroud, M., Gautier, N., Labbé, S. M., Villot, R., Turcotte, L., ... & Laplante, M. (2017). Loss of hepatic DEPTOR alters the metabolic transition to fasting. Molecular metabolism, 6(5), 447-458.

Hu, Y., Su, H., Liu, C., Wang, Z., Huang, L., Wang, Q., ... & Qing, G. (2017). DEPTOR is a direct NOTCH1 target that promotes cell proliferation and survival in T-cell leukemia. Oncogene, 36(8), 1038-1047.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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