Human DEPTOR ELISA Kit (2) (V2LY-0626-LY5733)

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Tested Data
Request for COA
Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity
0.21 ng/mL
Detection Range
0.38-24 ng/mL
Sample Type
Serum, Plasma, Urine, Ascites, Cerebrospinal fluid, Tissue
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
46.3 kDa
Components
  • Standard Solution: 0.5ml x1
  • Pre-coated ELISA Plate: 12 wells * 8 detachable strips
  • Standard Diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop Solution: 6ml x1
  • Substrate Solution A: 6ml x1
  • Substrate Solution B: 6ml x1
  • Wash Buffer Concentrate (25x): 20ml x1
  • Biotinylated Antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
DEP Domain Containing MTOR Interacting Protein
Function
Negative regulator of the mTORC1 and mTORC2 signaling pathways. Inhibits the kinase activity of both complexes.
Biological Process
Intracellular signal transduction Source: InterPro
Negative regulation of cell size Source: UniProtKB
Negative regulation of protein kinase activity Source: UniProtKB
Negative regulation of TOR signaling Source: UniProtKB
Regulation of extrinsic apoptotic signaling pathway Source: UniProtKB
PTM
Phosphorylated. Phosphorylation weakens interaction with MTOR within mTORC1 and mTORC2.
Ubiquitinated; ubiquitination leads to proteasomal degradation.

Wälchli, M., Berneiser, K., Mangia, F., Imseng, S., Craigie, L. M., Stuttfeld, E., ... & Maier, T. (2021). Regulation of human mTOR complexes by DEPTOR. Elife, 10, e70871.

Perez-Tejeiro, J. M., & Csukasi, F. (2021). DEPTOR in Skeletal Development and Diseases. Frontiers in Genetics, 12.

Heimhalt, M., Berndt, A., Wagstaff, J., Anandapadamanaban, M., Perisic, O., Maslen, S., ... & Williams, R. L. (2021). Bipartite binding and partial inhibition links DEPTOR and mTOR in a mutually antagonistic embrace. Elife, 10, e68799.

Chen, S., Jia, L., Zhang, S., Zheng, Y., & Zhou, Y. (2018). DEPTOR regulates osteogenic differentiation via inhibiting MEG3-mediated activation of BMP4 signaling and is involved in osteoporosis. Stem Cell Research & Therapy, 9(1), 1-14.

Varusai, T. M., & Nguyen, L. K. (2018). Dynamic modelling of the mTOR signalling network reveals complex emergent behaviours conferred by DEPTOR. Scientific reports, 8(1), 1-14.

Wang, Q., Zhou, Y., Rychahou, P., Harris, J. W., Zaytseva, Y. Y., Liu, J., ... & Evers, B. M. (2018). Deptor is a novel target of Wnt/β-catenin/c-Myc and contributes to colorectal cancer cell growth. Cancer research, 78(12), 3163-3175.

Chen, H., Shen, F., Sherban, A., Nocon, A., Li, Y., Wang, H., ... & Zang, M. (2018). DEP domain–containing mTOR–interacting protein suppresses lipogenesis and ameliorates hepatic steatosis and acute‐on‐chronic liver injury in alcoholic liver disease. Hepatology, 68(2), 496-514.

Caron, A., Briscoe, D. M., Richard, D., & Laplante, M. (2018). DEPTOR at the nexus of cancer, metabolism, and immunity. Physiological reviews, 98(3), 1765-1803.

Catena, V., & Fanciulli, M. (2017). Deptor: not only a mTOR inhibitor. Journal of Experimental & Clinical Cancer Research, 36(1), 1-9.

Caron, A., Mouchiroud, M., Gautier, N., Labbé, S. M., Villot, R., Turcotte, L., ... & Laplante, M. (2017). Loss of hepatic DEPTOR alters the metabolic transition to fasting. Molecular metabolism, 6(5), 447-458.

Hu, Y., Su, H., Liu, C., Wang, Z., Huang, L., Wang, Q., ... & Qing, G. (2017). DEPTOR is a direct NOTCH1 target that promotes cell proliferation and survival in T-cell leukemia. Oncogene, 36(8), 1038-1047.

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For research use only. Not intended for any clinical use.

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