Mouse Anti-DEPTOR Recombinant Antibody (D0708) (V2LY-0425-LY198)

Name: Mouse Anti-DEPTOR Recombinant Antibody (D0708)
SKU: V2LY-0425-LY198
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-DEPTOR Recombinant Antibody (D0708) (V2LY-0321-LY198-1) in WB

The western blotting assay was used to detect the expression of AKT signaling pathway-related molecules. ...View More

Huang, J., Li, J., Tang, J., Wu, Y., Dai, F., Yi, Z., ... & Xiang, T. (2022). ZDHHC22-mediated mTOR palmitoylation restrains breast cancer growth and endocrine therapy resistance. International journal of biological sciences, 18(7), 2833.

Mouse Anti-DEPTOR Recombinant Antibody (D0708) (V2LY-0321-LY198-2) in WB

Western blotting analysis of the effect of H19 on the expression of the components of mTORC1 pathway. Primary mouse hepatocytes were infected with Ad-H19, Ad-siH19 or Ad-RFP for 72 h. Total cell lysate was collected and subjected to Western blotting analysis with antibodies against members of the mTORC1 pathway. Levels of β-Actin were used as loading controls. ...View More

Wang, H., Cao, Y., Shu, L., Zhu, Y., Peng, Q., Ran, L., ... & Fan, J. (2020). Long non-coding RNA (lncRNA) H19 induces hepatic steatosis through activating MLXIPL and mTORC1 networks in hepatocytes. Journal of Cellular and Molecular Medicine, 24(2), 1399-1412.

Mouse Anti-DEPTOR Recombinant Antibody (D0708) (V2LY-0321-LY198-3) in IF

A-B. Immunofluorescence analysis of Deptor expression in plasma cells purified from primary
myelomas (A) and in MM cell lines (B). Cells were fixed in 4% formaldehyde for 15 min and then permeabilized with 0.1% Triton X-100. Nuclei were visualized by staining with Hoechst dye. Scale bar represents 10 μm; 63X original magnification. ...View More

Catena, V., Bruno, T., De Nicola, F., Goeman, F., Pallocca, M., Iezzi, S., ... & Fanciulli, M. (2016). Deptor transcriptionally regulates endoplasmic reticulum homeostasis in multiple myeloma cells. Oncotarget, 7(43), 70546.

Mouse Anti-DEPTOR Recombinant Antibody (D0708) (V2LY-0321-LY198-4) in WB

Western blot (WB)
analysis, with the indicated antibodies (Abs), of total (left), nuclear and cytoplasmic extracts (right) from KMS27, KMS18 and ARH77
MM cell lines. ...View More

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

D0708

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 18-37 near the N-terminus of human DEPTOR.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium Azide

Concentration

0.2 mg/ml

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

DEP Domain Containing MTOR Interacting Protein

Entrez Gene ID

Human	64798
Rat	314979
Mouse	97998

UniProt ID

Human	Q8TB45
Mouse	Q570Y9

Function

Negative regulator of the mTORC1 and mTORC2 signaling pathways. Inhibits the kinase activity of both complexes.

Biological Process

Intracellular signal transduction Source: InterPro
Negative regulation of cell size Source: UniProtKB
Negative regulation of protein kinase activity Source: UniProtKB
Negative regulation of TOR signaling Source: UniProtKB
Regulation of extrinsic apoptotic signaling pathway Source: UniProtKB

PTM

Phosphorylated. Phosphorylation weakens interaction with MTOR within mTORC1 and mTORC2.
Ubiquitinated; ubiquitination leads to proteasomal degradation.

Wälchli, M., Berneiser, K., Mangia, F., Imseng, S., Craigie, L. M., Stuttfeld, E., ... & Maier, T. (2021). Regulation of human mTOR complexes by DEPTOR. Elife, 10, e70871.

Perez-Tejeiro, J. M., & Csukasi, F. (2021). DEPTOR in Skeletal Development and Diseases. Frontiers in Genetics, 12.

Heimhalt, M., Berndt, A., Wagstaff, J., Anandapadamanaban, M., Perisic, O., Maslen, S., ... & Williams, R. L. (2021). Bipartite binding and partial inhibition links DEPTOR and mTOR in a mutually antagonistic embrace. Elife, 10, e68799.

Chen, S., Jia, L., Zhang, S., Zheng, Y., & Zhou, Y. (2018). DEPTOR regulates osteogenic differentiation via inhibiting MEG3-mediated activation of BMP4 signaling and is involved in osteoporosis. Stem Cell Research & Therapy, 9(1), 1-14.

Varusai, T. M., & Nguyen, L. K. (2018). Dynamic modelling of the mTOR signalling network reveals complex emergent behaviours conferred by DEPTOR. Scientific reports, 8(1), 1-14.

Wang, Q., Zhou, Y., Rychahou, P., Harris, J. W., Zaytseva, Y. Y., Liu, J., ... & Evers, B. M. (2018). Deptor is a novel target of Wnt/β-catenin/c-Myc and contributes to colorectal cancer cell growth. Cancer research, 78(12), 3163-3175.

Chen, H., Shen, F., Sherban, A., Nocon, A., Li, Y., Wang, H., ... & Zang, M. (2018). DEP domain–containing mTOR–interacting protein suppresses lipogenesis and ameliorates hepatic steatosis and acute‐on‐chronic liver injury in alcoholic liver disease. Hepatology, 68(2), 496-514.

Caron, A., Briscoe, D. M., Richard, D., & Laplante, M. (2018). DEPTOR at the nexus of cancer, metabolism, and immunity. Physiological reviews, 98(3), 1765-1803.

Catena, V., & Fanciulli, M. (2017). Deptor: not only a mTOR inhibitor. Journal of Experimental & Clinical Cancer Research, 36(1), 1-9.

Caron, A., Mouchiroud, M., Gautier, N., Labbé, S. M., Villot, R., Turcotte, L., ... & Laplante, M. (2017). Loss of hepatic DEPTOR alters the metabolic transition to fasting. Molecular metabolism, 6(5), 447-458.

Hu, Y., Su, H., Liu, C., Wang, Z., Huang, L., Wang, Q., ... & Qing, G. (2017). DEPTOR is a direct NOTCH1 target that promotes cell proliferation and survival in T-cell leukemia. Oncogene, 36(8), 1038-1047.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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