Search :
Sign in or Register  
Welcome Sign in or Don't have an account?Register

Mouse Anti-DEPTOR Recombinant Antibody (A2189) (V2LY-0425-LY200)


See all DEPTOR antibodies
Online Inquiry

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
A2189
Antibody Isotype
IgG1, κ
Application
WB, IP, IF, ELISA

Basic Information

Immunogen
Amino acids 1-50 mapping at the N-terminus of human DEPTOR.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
Gelatin & PBS
Preservative
Sodium Azide
Concentration
0.2 mg/ml
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
DEP Domain Containing MTOR Interacting Protein
Entrez Gene ID
Human64798
Rat314979
Mouse97998
UniProt ID
HumanQ8TB45
MouseQ570Y9
Function
Negative regulator of the mTORC1 and mTORC2 signaling pathways. Inhibits the kinase activity of both complexes.
Biological Process
Intracellular signal transduction Source: InterPro
Negative regulation of cell size Source: UniProtKB
Negative regulation of protein kinase activity Source: UniProtKB
Negative regulation of TOR signaling Source: UniProtKB
Regulation of extrinsic apoptotic signaling pathway Source: UniProtKB
PTM
Phosphorylated. Phosphorylation weakens interaction with MTOR within mTORC1 and mTORC2.
Ubiquitinated; ubiquitination leads to proteasomal degradation.
More Infomation

Wälchli, M., Berneiser, K., Mangia, F., Imseng, S., Craigie, L. M., Stuttfeld, E., ... & Maier, T. (2021). Regulation of human mTOR complexes by DEPTOR. Elife, 10, e70871.

Perez-Tejeiro, J. M., & Csukasi, F. (2021). DEPTOR in Skeletal Development and Diseases. Frontiers in Genetics, 12.

Heimhalt, M., Berndt, A., Wagstaff, J., Anandapadamanaban, M., Perisic, O., Maslen, S., ... & Williams, R. L. (2021). Bipartite binding and partial inhibition links DEPTOR and mTOR in a mutually antagonistic embrace. Elife, 10, e68799.

Chen, S., Jia, L., Zhang, S., Zheng, Y., & Zhou, Y. (2018). DEPTOR regulates osteogenic differentiation via inhibiting MEG3-mediated activation of BMP4 signaling and is involved in osteoporosis. Stem Cell Research & Therapy, 9(1), 1-14.

Varusai, T. M., & Nguyen, L. K. (2018). Dynamic modelling of the mTOR signalling network reveals complex emergent behaviours conferred by DEPTOR. Scientific reports, 8(1), 1-14.

Wang, Q., Zhou, Y., Rychahou, P., Harris, J. W., Zaytseva, Y. Y., Liu, J., ... & Evers, B. M. (2018). Deptor is a novel target of Wnt/β-catenin/c-Myc and contributes to colorectal cancer cell growth. Cancer research, 78(12), 3163-3175.

Chen, H., Shen, F., Sherban, A., Nocon, A., Li, Y., Wang, H., ... & Zang, M. (2018). DEP domain–containing mTOR–interacting protein suppresses lipogenesis and ameliorates hepatic steatosis and acute‐on‐chronic liver injury in alcoholic liver disease. Hepatology, 68(2), 496-514.

Caron, A., Briscoe, D. M., Richard, D., & Laplante, M. (2018). DEPTOR at the nexus of cancer, metabolism, and immunity. Physiological reviews, 98(3), 1765-1803.

Catena, V., & Fanciulli, M. (2017). Deptor: not only a mTOR inhibitor. Journal of Experimental & Clinical Cancer Research, 36(1), 1-9.

Caron, A., Mouchiroud, M., Gautier, N., Labbé, S. M., Villot, R., Turcotte, L., ... & Laplante, M. (2017). Loss of hepatic DEPTOR alters the metabolic transition to fasting. Molecular metabolism, 6(5), 447-458.

Hu, Y., Su, H., Liu, C., Wang, Z., Huang, L., Wang, Q., ... & Qing, G. (2017). DEPTOR is a direct NOTCH1 target that promotes cell proliferation and survival in T-cell leukemia. Oncogene, 36(8), 1038-1047.

Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Mouse Anti-DEPTOR Recombinant Antibody (A2189)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Learn more

Documents

Request bulk or custom quote Online Inquiry
Second antibody and isotype control

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Online Inquiry