Mouse Anti-PCK2 Recombinant Antibody (CBYC-P219) (CBMAB-P1055-YC)

Name: Mouse Anti-PCK2 Recombinant Antibody (CBYC-P219)
SKU: CBMAB-P1055-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBYC-P219

Application

WB, ICC, IHC-P, IHC-Fr, ELISA

Immunogen

Phosphoenolpyruvate Carboxykinase 2, Mitochondrial

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

PBS, pH 7.4, 0.02% sodium azide, 50% glycerol

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Phosphoenolpyruvate Carboxykinase 2, Mitochondrial

Introduction

PCK2 is a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of guanosine triphosphate (GTP). A cytosolic form of this protein is encoded by a different gene and is the key enzyme of gluconeogenesis in the liver. Alternatively spliced transcript variants have been described.

Entrez Gene ID

5106

UniProt ID

Q16822

Alternative Names

PEPCK; PEPCK-M; PEPCK2

Function

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.

Biological Process

Cellular response to dexamethasone stimulusManual Assertion Based On ExperimentIBA:GO_Central
Cellular response to glucose stimulusManual Assertion Based On ExperimentIBA:GO_Central
Cellular response to insulin stimulusManual Assertion Based On ExperimentIBA:GO_Central
Cellular response to tumor necrosis factorIEA:Ensembl
GluconeogenesisManual Assertion Based On ExperimentIBA:GO_Central
Glycerol biosynthetic process from pyruvateManual Assertion Based On ExperimentIBA:GO_Central
Hepatocyte differentiationManual Assertion Based On ExperimentIBA:GO_Central
NADH oxidationIEA:Ensembl
Oxaloacetate metabolic processIEA:Ensembl
Positive regulation of insulin secretionIEA:Ensembl
Propionate catabolic processManual Assertion Based On ExperimentIBA:GO_Central
Response to lipidManual Assertion Based On ExperimentIBA:GO_Central
Response to lipopolysaccharideIEA:Ensembl
Response to starvationManual Assertion Based On ExperimentIBA:GO_Central

Cellular Location

Mitochondrion

Involvement in disease

Mitochondrial phosphoenolpyruvate carboxykinase deficiency (M-PEPCKD):
Metabolic disorder resulting from impaired gluconeogenesis. It is a rare disease with less than 10 cases reported in the literature. Clinical characteristics include hypotonia, hepatomegaly, failure to thrive, lactic acidosis and hypoglycemia. Autopsy reveals fatty infiltration of both the liver and kidneys. The disorder is transmitted as an autosomal recessive trait.

Xue, S., Cai, Y., Liu, J., Ji, K., Yi, P., Long, H., ... & Song, Y. (2024). Dysregulation of phosphoenolpyruvate carboxykinase in cancers: A comprehensive analysis. Cellular Signalling, 120, 111198.

Hsu, H. P., Chu, P. Y., Chang, T. M., Huang, K. W., Hung, W. C., Jiang, S. S., ... & Tsai, H. J. (2023). Mitochondrial phosphoenolpyruvate carboxykinase promotes tumor growth in estrogen receptor‐positive breast cancer via regulation of the mTOR pathway. Cancer medicine, 12(2), 1588-1601.

Yu, S., Meng, S., Xiang, M., & Ma, H. (2021). Phosphoenolpyruvate carboxykinase in cell metabolism: Roles and mechanisms beyond gluconeogenesis. Molecular metabolism, 53, 101257.

Geng, X., Shen, J., Li, F., Yip, J., Guan, L., Rajah, G., ... & Ding, Y. (2021). Phosphoenolpyruvate carboxykinase (PCK) in the brain gluconeogenic pathway contributes to oxidative and lactic injury after stroke. Molecular Neurobiology, 58, 2309-2321.

Dong, H., Feng, Y., Yang, Y., Hu, Y., Jia, Y., Yang, S., ... & Zhao, R. (2021). A novel function of mitochondrial phosphoenolpyruvate carboxykinase as a regulator of inflammatory response in Kupffer cells. Frontiers in Cell and Developmental Biology, 9, 726931.

Li, Z., Liu, X., Zhu, Y., Du, Y., Liu, X., Lv, L., ... & Zhou, Y. (2019). Mitochondrial phosphoenolpyruvate carboxykinase regulates osteogenic differentiation by modulating AMPK/ULK1-dependent autophagy. Stem Cells, 37(12), 1542-1555.

Seenappa, V., Joshi, M. B., & Satyamoorthy, K. (2019). Intricate regulation of phosphoenolpyruvate carboxykinase (PEPCK) isoforms in normal physiology and disease. Current Molecular Medicine, 19(4), 247-272.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Antibody Pairs

PCK2 Matched Antibody Pair (848) (CAT#: APMAB-848LY)

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Isotype control

For research use only. Not intended for any clinical use.

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