Human PCK2 ELISA Kit (V2LY-0626-LY5023)

See all PCK2 antibodies
Go to compare Compare Online Inquiry
Tested Data
Datasheet
SDS
Request for COA
Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity
0.007 ng/mL
Detection Range
0.015-3 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
70.7 kDa
Components
  • Pre-coated ELISA plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
Phosphoenolpyruvate Carboxykinase 2, Mitochondrial
Function
Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.
Biological Process
Cellular response to dexamethasone stimulusManual Assertion Based On ExperimentIBA:GO_Central
Cellular response to glucose stimulusManual Assertion Based On ExperimentIBA:GO_Central
Cellular response to insulin stimulusManual Assertion Based On ExperimentIBA:GO_Central
Cellular response to tumor necrosis factorIEA:Ensembl
GluconeogenesisManual Assertion Based On ExperimentIBA:GO_Central
Glycerol biosynthetic process from pyruvateManual Assertion Based On ExperimentIBA:GO_Central
Hepatocyte differentiationManual Assertion Based On ExperimentIBA:GO_Central
NADH oxidationIEA:Ensembl
Oxaloacetate metabolic processIEA:Ensembl
Positive regulation of insulin secretionIEA:Ensembl
Propionate catabolic processManual Assertion Based On ExperimentIBA:GO_Central
Response to lipidManual Assertion Based On ExperimentIBA:GO_Central
Response to lipopolysaccharideIEA:Ensembl
Response to starvationManual Assertion Based On ExperimentIBA:GO_Central
Cellular Location
Mitochondrion
Involvement in disease
Mitochondrial phosphoenolpyruvate carboxykinase deficiency (M-PEPCKD):
Metabolic disorder resulting from impaired gluconeogenesis. It is a rare disease with less than 10 cases reported in the literature. Clinical characteristics include hypotonia, hepatomegaly, failure to thrive, lactic acidosis and hypoglycemia. Autopsy reveals fatty infiltration of both the liver and kidneys. The disorder is transmitted as an autosomal recessive trait.

Xue, S., Cai, Y., Liu, J., Ji, K., Yi, P., Long, H., ... & Song, Y. (2024). Dysregulation of phosphoenolpyruvate carboxykinase in cancers: A comprehensive analysis. Cellular Signalling, 120, 111198.

Hsu, H. P., Chu, P. Y., Chang, T. M., Huang, K. W., Hung, W. C., Jiang, S. S., ... & Tsai, H. J. (2023). Mitochondrial phosphoenolpyruvate carboxykinase promotes tumor growth in estrogen receptor‐positive breast cancer via regulation of the mTOR pathway. Cancer medicine, 12(2), 1588-1601.

Yu, S., Meng, S., Xiang, M., & Ma, H. (2021). Phosphoenolpyruvate carboxykinase in cell metabolism: Roles and mechanisms beyond gluconeogenesis. Molecular metabolism, 53, 101257.

Geng, X., Shen, J., Li, F., Yip, J., Guan, L., Rajah, G., ... & Ding, Y. (2021). Phosphoenolpyruvate carboxykinase (PCK) in the brain gluconeogenic pathway contributes to oxidative and lactic injury after stroke. Molecular Neurobiology, 58, 2309-2321.

Dong, H., Feng, Y., Yang, Y., Hu, Y., Jia, Y., Yang, S., ... & Zhao, R. (2021). A novel function of mitochondrial phosphoenolpyruvate carboxykinase as a regulator of inflammatory response in Kupffer cells. Frontiers in Cell and Developmental Biology, 9, 726931.

Li, Z., Liu, X., Zhu, Y., Du, Y., Liu, X., Lv, L., ... & Zhou, Y. (2019). Mitochondrial phosphoenolpyruvate carboxykinase regulates osteogenic differentiation by modulating AMPK/ULK1-dependent autophagy. Stem Cells, 37(12), 1542-1555.

Seenappa, V., Joshi, M. B., & Satyamoorthy, K. (2019). Intricate regulation of phosphoenolpyruvate carboxykinase (PEPCK) isoforms in normal physiology and disease. Current Molecular Medicine, 19(4), 247-272.

Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Human PCK2 ELISA Kit?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant PCK2 protein, GST Tag (CAT#: V2LY-0526-LY5987)

PCK2 Matched Antibody Pair (848) (CAT#: APMAB-848LY)

Antibody Pairs

PCK2 Matched Antibody Pair (848) (CAT#: APMAB-848LY)

Related Products

Mouse Anti-PCK2 Recombinant Antibody (CBT1018) (CAT#: V2LY-0625-LY3104)

Mouse Anti-PCK2 Recombinant Antibody (CBT3818) (CAT#: V2LY-0625-LY1191)

Mouse Anti-PCK2 Recombinant Antibody (2C9) (CAT#: CBMAB-P1052-YC)

PCK2 Matched Antibody Pair (848) (CAT#: APMAB-848LY)

Mouse Anti-PCK2 Recombinant Antibody (CBT2074) (CAT#: V2LY-0625-LY1240)

Rabbit Anti-PCK2 Recombinant Antibody (D3E11) (CAT#: CBMAB-P1053-YC)

Mouse Anti-PCK2 Recombinant Antibody (CBYC-P219) (CAT#: CBMAB-P1055-YC)

Human Recombinant PCK2 protein, GST Tag (CAT#: V2LY-0526-LY5987)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry
Request bulk or custom quote Second antibody and isotype control

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

online inquiry
Online Inquiry

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.