Human Anti-TMEFF2 Recombinant Antibody (
6G6) (V2LY-0725-LY1432)

Name: Human Anti-TMEFF2 Recombinant Antibody (6G6)
SKU: V2LY-0725-LY1432
Rating: 5 (1 reviews)

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Tested Data

Human Anti-TMEFF2 Recombinant Antibody (6G6) (V2LY-0725-LY1432) in ELISA

The Binding Activity of TMEFF2 with Anti-TMEFF2 monoclonal antibodyActivity: Measured by its binding ability in a functional ELISA. Immobilized Human TMEFF2 at 2 μg/mL can bind Anti-TMEFF2 recombinant antibody, the EC50 is 2.129-2.956 ng/mL.

Datasheet

SDS

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Basic Information

Host Animal

Human

Clone

6G6

Application

ELISA

Immunogen

Recombinant Human TMEFF2 protein.

Host Species

Human

Specificity

Human

Antibody Isotype

IgG4

Clonality

Monoclonal Antibody

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, Glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

transmembrane protein with EGF-like and two follistatin-like domains 2

Entrez Gene ID

23671

UniProt ID

Q9UIK5

Function

May be a survival factor for hippocampal and mesencephalic neurons. The shedded form up-regulates cancer cell proliferation, probably by promoting ERK1/2 phosphorylation.

Biological Process

Biological Process animal organ morphogenesisSource:GO_Central1 Publication
Biological Process cell migrationSource:GO_Central1 Publication
Biological Process negative regulation of cell migrationSource:MGI1 Publication
Biological Process negative regulation of integrin biosynthetic processSource:MGI1 Publication
Biological Process negative regulation of stress fiber assemblySource:MGI1 Publication
Biological Process substrate adhesion-dependent cell spreadingSource:GO_Central1 Publication
Biological Process tissue developmentSource:GO_Central1 Publication
Biological Process wound healing, spreading of cellsSource:MGI1 Publication

Cellular Location

Isoform 1
Membrane
Isoform 2
Membrane
Isoform 3
Secreted

Topology

Extracellular: 41-320
Helical: 321-341
Cytoplasmic: 342-374

PTM

N-glycosylated. Contains chondroitin sulfate glycosaminoglycans.
A soluble form (TMEFF2-ECD) is produced by proteolytic shedding. This shedding can be induced by phorbol ester or pro-inflammatory cytokines such as TNFalpha, and is mediated by ADAM17.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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