Human DKKL1 ELISA Kit (V2LY-0626-LY3652)

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Tested Data
Datasheet
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Basic Information

Sensitivity
0.018 ng/mL
Detection Range
0.05-15 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
27.0 kDa
Components
  • Pre-coated ELISA Plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
dickkopf-like 1
Function
Involved in fertilization by facilitating sperm penetration of the zona pellucida. May promote spermatocyte apoptosis, thereby limiting sperm production. In adults, may reduce testosterone synthesis in Leydig cells. Is not essential either for development or fertility.
Biological Process
Anatomical structure morphogenesis Source: ProtInc
Negative regulation of canonical Wnt signaling pathway Source: GO_Central
Negative regulation of testosterone biosynthetic process Source: UniProtKB
Penetration of zona pellucida Source: UniProtKB
Positive regulation of apoptotic process Source: UniProtKB
Positive regulation of fat cell differentiation Source: Ensembl
Cellular Location
Secreted; Acrosome. Localized specifically to the crescent shaped acrosome at the apex of the sperm head.
PTM
N-glycosylated during spermatogenesis. Not N-glycosylated in mature sperm.

Yan, Q. X., Ma, Y., Chen, R. Q., Zhou, X. Q., Qiao, J., Xian, Y. J., ... & Chen, C. R. (2018). Expression of DKKL1 in spermatozoa of men with asthenospermia. Nan Fang yi ke da xue xue bao= Journal of Southern Medical University, 38(3), 324-328.

Martin, G., Selcuklu, S. D., Schouest, K., Nembaware, V., McKeown, P. C., Seoighe, C., & Spillane, C. (2017). Allele-specific splicing effects on DKKL1 and ZNF419 transcripts in HeLa cells. Gene, 598, 107-112.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Mouse Dkkl1 ELISA Kit (CAT#: V2LY-0626-LY1488)

Human Recombinant DKKL1 protein, hFc Tag (CAT#: V2LY-0526-LY3585)

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Mouse Anti-DKKL1 Recombinant Antibody (6G10) (CAT#: CBMAB-D1004-YC)

Mouse Anti-DKKL1 Recombinant Antibody (CBXS-2278) (CAT#: V2LY-0425-LY410)

Mouse Dkkl1 ELISA Kit (CAT#: V2LY-0626-LY1488)

Mouse Anti-DKKL1 Recombinant Antibody (CBXS-5832) (CAT#: V2LY-0425-LY409)

Mouse Anti-DKKL1 Recombinant Antibody (CBXS-2278) (CAT#: CBMAB-S5040-CQ)

Rat Anti-DKKL1 Recombinant Antibody (CBXS-5633) (CAT#: V2LY-0425-LY408)

Mouse Anti-DKKL1 Recombinant Antibody (D1004) (CAT#: V2LY-0425-LY407)

Human Recombinant DKKL1 protein, hFc Tag (CAT#: V2LY-0526-LY3585)

Mouse Anti-DKKL1 Recombinant Antibody (CBXS-5832) (CAT#: CBMAB-S0687-CQ)

Isotype control

For research use only. Not intended for any clinical use.

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