Human Recombinant DKKL1 protein, hFc Tag (V2LY-0526-LY3585)

Name: Human Recombinant DKKL1 protein, hFc Tag
SKU: V2LY-0526-LY3585
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

hFc Tag

Protein Construction

This product is Human Recombinant DKKL1 protein, hFc Tag consist of Amino Acid: 31-242 and predicts a molecular mass of 52.4 kDa.

Molecule Mass

52.4 kDa

Sequence

Amino Acid: 31-242

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>70% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

dickkopf-like 1

Function

Involved in fertilization by facilitating sperm penetration of the zona pellucida. May promote spermatocyte apoptosis, thereby limiting sperm production. In adults, may reduce testosterone synthesis in Leydig cells. Is not essential either for development or fertility.

Biological Process

Anatomical structure morphogenesis Source: ProtInc
Negative regulation of canonical Wnt signaling pathway Source: GO_Central
Negative regulation of testosterone biosynthetic process Source: UniProtKB
Penetration of zona pellucida Source: UniProtKB
Positive regulation of apoptotic process Source: UniProtKB
Positive regulation of fat cell differentiation Source: Ensembl

Cellular Location

Secreted; Acrosome. Localized specifically to the crescent shaped acrosome at the apex of the sperm head.

PTM

N-glycosylated during spermatogenesis. Not N-glycosylated in mature sperm.

Yan, Q. X., Ma, Y., Chen, R. Q., Zhou, X. Q., Qiao, J., Xian, Y. J., ... & Chen, C. R. (2018). Expression of DKKL1 in spermatozoa of men with asthenospermia. Nan Fang yi ke da xue xue bao= Journal of Southern Medical University, 38(3), 324-328.

Martin, G., Selcuklu, S. D., Schouest, K., Nembaware, V., McKeown, P. C., Seoighe, C., & Spillane, C. (2017). Allele-specific splicing effects on DKKL1 and ZNF419 transcripts in HeLa cells. Gene, 598, 107-112.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Mouse Dkkl1 ELISA Kit (CAT#: V2LY-0626-LY1488)

Human DKKL1 ELISA Kit (CAT#: V2LY-0626-LY3652)

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Mouse Anti-DKKL1 Recombinant Antibody (CBXS-2278) (CAT#: CBMAB-S5040-CQ)

Mouse Anti-DKKL1 Recombinant Antibody (CBXS-2278) (CAT#: V2LY-0425-LY410)

Mouse Anti-DKKL1 Recombinant Antibody (D1004) (CAT#: V2LY-0425-LY407)

Mouse Anti-DKKL1 Recombinant Antibody (CBXS-5832) (CAT#: CBMAB-S0687-CQ)

Rat Anti-DKKL1 Recombinant Antibody (CBXS-5633) (CAT#: V2LY-0425-LY408)

Mouse Dkkl1 ELISA Kit (CAT#: V2LY-0626-LY1488)

Rat Anti-DKKL1 Recombinant Antibody (CBXS-5633) (CAT#: CBMAB-S0510-CQ)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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