HLA-DRB1 Antibodies

Structure of HLA-DRB1

The HLA-DRB1 gene is a key gene located in the MHC II region on human chromosome 6. The protein encoded by this gene typically has a molecular weight ranging from 25 to 33 kDa. The specific value depends on different allele types and their post-translational modifications.

The protein encoded by this gene consists of approximately 266 amino acids. Its three-dimensional structure features an open antigen-binding groove composed of α helices and β sheets. The core functional region of this protein is the antigen-binding site formed by its secondary structure (the "bottom" composed of β sheets and the "side wall" composed of α helices), which can specifically bind and present exogenous peptide segments. The high polymorphism on the protein surface is mainly concentrated on the amino acid residues of the antigen-binding groove, and these differences directly determine the specificity of immune recognition and disease susceptibility in different individuals.

Fig. 1 HLA-DRB1 gene is encoded by six exons.¹

Key structural properties of HLA-DRB1:

• Composed of alpha helix with beta fold open the antigen slots
• Highly polymorphic amino acid residues line the antigen-binding groove surface
• The peptide binding domain realizes the specific recognition and stable binding of foreign antigen through the hydrogen bond network

Functions of HLA-DRB1

The core function of the HLA-DRB1 gene is to present antigenic peptide segments during the adaptive immune response. However, it is also deeply involved in various immune regulatory processes, including the establishment of self-immune tolerance and the regulation of the intensity of immune responses.

The HLA-DRB1 protein has a highly selective but relatively low affinity for antigenic peptide segments. This is in line with the biological requirement of presenting multiple different antigens, ensuring that the immune system can extensively monitor and respond flexibly to constantly changing pathogen threats.

Applications of HLA-DRB1 and HLA-DRB1 Antibody in Literature

1. Brynedal, Boel, et al. "HLA-A confers an HLA-DRB1 independent influence on the risk of multiple sclerosis." PloS one 2.7 (2007): e664. https://doi.org/10.1371/journal.pone.0000664

This study found that the HLA-A gene has an independent effect on the risk of multiple sclerosis (MS) that is separate from the HLA-DRB1 gene. Among the 1084 MS patients and 1347 control subjects, the HLA-A*02 allele significantly reduced the risk. The results confirmed that the genes in the HLA class I region have an additional effect on the onset of MS, suggesting that both the regulation and effect mechanisms of the immune system are involved in the occurrence of MS.

2. Wysocki, Tomasz, Marzena Olesińska, and Agnieszka Paradowska-Gorycka. "Current understanding of an emerging role of HLA-DRB1 gene in rheumatoid arthritis–from research to clinical practice." Cells 9.5 (2020): 1127. https://doi.org/10.3390/cells9051127

The article indicates that the onset of rheumatoid arthritis is closely related to the HLA-DRB1 gene. This gene affects the structure of immune molecules, antigen binding, and non-joint symptoms, and is also related to the treatment response. A deeper understanding of its mechanism of action is conducive to promoting the development of personalized diagnosis and treatment for RA.

3. Danquah, Bright D., et al. "Mass Spectrometric analysis of antibody—Epitope peptide complex dissociation: Theoretical concept and practical procedure of binding strength characterization." Molecules 25.20 (2020): 4776. https://doi.org/10.1186/ar1837

This study compared the HLA-DRB1/DQB1 genes of 159 RA patients with severe extra-articular manifestations and 178 RA patients without such manifestations. The results showed that only Felty syndrome was significantly associated with HLA-DRB1*0401; other extra-articular manifestations were mainly related to the DRB1*04 genotype carrying double-dose shared epitopes.

4. Alcina, Antonio, et al. "Multiple sclerosis risk variant HLA-DRB1* 1501 associates with high expression of DRB1 gene in different human populations." PloS one 7.1 (2012): e29819. https://doi.org/10.1371/journal.pone.0029819

Studies have shown that the risk of multiple sclerosis is closely related to HLA-DRB1*1501. The expression levels of genes such as DRB1 and DQB1 in carriers of the risk genotype are significantly increased. Research indicates that the high expression of specific alleles, together with their structural characteristics, jointly drive the susceptibility to the disease, rather than being solely determined by antigen presentation.

5. Liu, Mohan, et al. "Identification of HLA-DRB1 association to adalimumab immunogenicity." PLoS One 13.4 (2018): e0195325. https://doi.org/10.1371/journal.pone.0195325

This study, through HLA typing, found that HLA-DRB1*03 and HLA-DRB1*011 could increase the risk of adalimumab-resistant antibodies (AAA) formation, while HLA-DRB1*01 and HLA-DRB1*07 had protective effects. This indicates that specific HLA alleles play a crucial role in antibody production.

Creative Biolabs: HLA-DRB1 Antibodies for Research

Creative Biolabs specializes in the production of high-quality HLA-DRB1 antibodies for research and industrial applications. Our portfolio includes monoclonal antibodies tailored for ELISA, Flow Cytometry, Western blot, immunohistochemistry, and other diagnostic methodologies.

• Custom HLA-DRB1 Antibody Development: Tailor-made solutions to meet specific research requirements.
• Bulk Production: Large-scale antibody manufacturing for industry partners.
• Technical Support: Expert consultation for protocol optimization and troubleshooting.
• Aliquoting Services: Conveniently sized aliquots for long-term storage and consistent experimental outcomes.

For more details on our HLA-DRB1 antibodies, custom preparations, or technical support, contact us at email.