MCM2 Antibodies

Structure of MCM2

MCM2 is a core subunit of the replicative helicase with a molecular weight of approximately 110 kDa. This weight can vary due to post-translational modifications such as phosphorylation across different species.

The MCM2 protein contains over 800 amino acids and assembles into a characteristic ring-shaped hexameric structure through its conserved AAA+ ATPase domain. The protein structure of MCM2 features a central channel that directly encircles double-stranded DNA, which is driven by ATP hydrolysis to unwind DNA at the replication fork. The protein's function is critically regulated by its N-terminal domain, which mediates interactions with other MCM subunits and regulatory proteins like Cdc45. MCM2 displays a secondary structure rich in alpha-helices and beta-strands that form distinct oligonucleotide-binding folds for ATP binding. The arginine finger motif is essential for inter-subunit communication and transmission of the ATP hydrolysis signal throughout the helicase complex.

Fig. 1 Potential mechanisms of MCM2 in promoting cancer progression.¹

Key structural properties of MCM2:

• Conserved AAA+ ATPase domain
• Extended N-terminal domain
• Zinc finger motif and arginine finger motif

Functions of MCM2

The core function of the MCM2 gene is to serve as a DNA replication license factor and a core component of helicase. In addition, it is also involved in a variety of cellular processes, including replication stress responses and cell cycle regulation.

The loading of MCM2 during the replication licensing stage is highly controlled, while its activation in the S phase is an irreversible process. This ensures precise single replication of the eukaryotic genome, and its dysfunction is closely related to genomic instability and tumorigenesis.

Applications of MCM2 and MCM2 Antibody in Literature

1. Sun, Yaoqi, Zhong Cheng, and Shupeng Liu. "MCM2 in human cancer: functions, mechanisms, and clinical significance." Molecular medicine 28.1 (2022): 128. https://doi.org/10.1186/s10020-022-00555-9

The article indicates that MCM2 is a core protein for DNA replication and is overexpressed in various tumors. Its abnormal function is associated with cancer progression and poor prognosis. MCM2 can serve as a biomarker for diagnosis, prognosis and chemotherapy response, and its inhibitors also have potential for anti-tumor and synergistic immunotherapy.

2. Rankin, Brooke D., and Susannah Rankin. "The MCM2-7 Complex: Roles beyond DNA Unwinding." Biology 13.4 (2024): 258. https://doi.org/10.3390/biology13040258

The article indicates that the MCM2-7 complex is not only a key helicase in DNA replication, but also participates in various core cellular activities such as genome folding, histone inheritance and DNA damage repair. These functions make it play a crucial role in maintaining genomic stability.

3. Reuter, L. Maximilian, et al. "MCM2-7 loading-dependent ORC release ensures genome-wide origin licensing." Nature Communications 15.1 (2024): 7306. https://doi.org/10.1038/s41467-024-51538-9

This study reveals the core mechanism by which MCM2-7 helicase is loaded to the replication starting point on a genomic scale: the loading of a single MCM2-7 bishexamer prevents ORC from rebinding through steric hindrance, thereby precisely regulating DNA replication permission. In addition, specific DNA structural features and element spacing are the keys to efficient loading.

4. Simon, Nicholas E., and Anthony Schwacha. "The Mcm2‐7 replicative helicase: a promising chemotherapeutic target." BioMed research international 2014.1 (2014): 549719. https://doi.org/10.1155/2014/549719

The article indicates that Mcm2-7 helicase is the core of DNA replication initiation and extension, and is associated with various squamous cell carcinomas. As a key link connecting replication and regulation, the discovery of its small molecule inhibitors has made it a highly promising new chemotherapy target.

5. Xu X, Hua X, et al. "Mcm2 promotes stem cell differentiation via its ability to bind H3-H4." elife 11 (2022): e80917. https://doi.org/10.7554/eLife.80917

Research has found that, in addition to the known DNA replication function, the histone binding ability of Mcm2 is crucial for the differentiation of embryonic stem cells. This function shapes the chromatin landscape during cell differentiation by influencing chromatin accessibility, regulating pluripotent gene silencing and linage-specific gene activation.

Creative Biolabs: MCM2 Antibodies for Research

Creative Biolabs specializes in the production of high-quality MCM2 antibodies for research and industrial applications. Our portfolio includes monoclonal antibodies tailored for ELISA, Flow Cytometry, Western blot, immunohistochemistry, and other diagnostic methodologies.

• Custom MCM2 Antibody Development: Tailor-made solutions to meet specific research requirements.
• Bulk Production: Large-scale antibody manufacturing for industry partners.
• Technical Support: Expert consultation for protocol optimization and troubleshooting.
• Aliquoting Services: Conveniently sized aliquots for long-term storage and consistent experimental outcomes.

For more details on our MCM2 antibodies, custom preparations, or technical support, contact us at email.