NLRP3 Antibodies

Structure of NLRP3

NLRP3 is an inflammasome sensor protein with a molecular weight of approximately 118 kDa, and its molecular weight varies to some extent among different species. The following is a comparison of NLRP3 characteristics of major species:

This protein achieves oligomerization through the ATPase activity of its NACHT domain, forming an inflammasome core. The PYD domain is responsible for interacting with the adaptor protein ASC, while the LRR domain is involved in ligand recognition. The activation of NLRP3 requires two signals: the initiation signal (such as TLR ligands) and the activation signal (such as ATP or crystals). This dual regulatory mechanism ensures the precise control of inflammatory responses.

Fig. 1 Schematic illustration of the NLRP3 inflammasome activation.¹

Key structural properties of NLRP3:

• Multi-domain composition (PYD-NACHT-LRR)
• Atpase activity NACHT structure domain
• LRR structure domain is responsible for the pathogen recognition
• PYD domains mediate protein interactions

Functions of NLRP3

The core function of NLRP3 is to serve as a danger signal sensor for the innate immune system and simultaneously participate in multiple pathophysiological processes:

Applications of NLRP3 and NLRP3 Antibody in Literature

1. Shao, Bo-Zong, et al. "NLRP3 inflammasome and its inhibitors: a review." Frontiers in pharmacology 6 (2015): 262. https://doi.org/10.3389/fphar.2015.00262

The article indicates that NLRP3 antibodies target key proteins of inflammasomes, regulate innate immunity, and are closely related to metabolic disorders, autoimmune diseases, etc. Current research focuses on its structure, activation mechanism and inhibitor development, providing new strategies for the treatment of related diseases.

2. Moossavi, Maryam, et al. "Role of the NLRP3 inflammasome in cancer." Molecular cancer 17.1 (2018): 158. https://doi.org/10.1186/s12943-018-0900-3

The article indicates that the NLRP3 antibody targets inflammasomes, regulates the activation of caspase-1 and the release of IL-1β/IL-18, and affects tumorigenesis and development. Its role in cancer remains controversial, but it is a potential therapeutic target.

3. Yang, Yang, et al. "Recent advances in the mechanisms of NLRP3 inflammasome activation and its inhibitors." Cell death & disease 10.2 (2019): 128.https://doi.org/10.1038/s41419-019-1413-8

The article indicates that the NLRP3 antibody regulates the release of IL-1β/IL-18 by targeting the inflammasome complex and participates in the disease processes such as Alzheimer's disease and diabetes. Studying its activation mechanism and specific inhibitors is of great significance for the treatment of related diseases.

4. Zhen, Yu, and Hu Zhang. "NLRP3 inflammasome and inflammatory bowel disease." Frontiers in immunology 10 (2019): 276. https://doi.org/10.3389/fimmu.2019.00276

The article indicates that NLRP3 antibody regulates caspase-1 activation by targeting the PYD domain of the inflammasome and affects the secretion of IL-1β/IL-18, exerting a dual role (pro-inflammatory/protective) in intestinal mucosal immunity in IBD. The study of its mechanism provides a new target for the treatment of IBD.

5. Bai, Baochen, et al. "NLRP3 inflammasome in endothelial dysfunction." Cell death & disease 11.9 (2020): 776. https://doi.org/10.1038/s41419-020-02985-x

The article indicates that the NLRP3 antibody targets inflammasomes, inhibits Caspase-1-mediated IL-1β/IL-18 release and pyroptosis, improves oxidative stress and endothelial dysfunction, and provides a new strategy for the treatment of cardiovascular diseases.

Creative Biolabs: NLRP3 Antibodies for Research

Creative Biolabs specializes in the production of high-quality NLRP3 antibodies for research and industrial applications. Our portfolio includes monoclonal antibodies tailored for ELISA, Flow Cytometry, Western blot, immunohistochemistry, and other diagnostic methodologies.

• Custom NLRP3 Antibody Development: Tailor-made solutions to meet specific research requirements.
• Bulk Production: Large-scale antibody manufacturing for industry partners.
• Technical Support: Expert consultation for protocol optimization and troubleshooting.
• Aliquoting Services: Conveniently sized aliquots for long-term storage and consistent experimental outcomes.

For more details on our NLRP3 antibodies, custom preparations, or technical support, contact us at email.