PARP3

The protein encoded by this gene belongs to the PARP family. These enzymes modify nuclear proteins by poly-ADP-ribosylation, which is required for DNA repair, regulation of apoptosis, and maintenance of genomic stability. This gene encodes the poly(ADP-ribosyl)transferase 3, which is preferentially localized to the daughter centriole throughout the cell cycle. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]

poly (ADP-ribose) polymerase family, member 3

Mono-ADP-ribosyltransferase that mediates mono-ADP-ribosylation of target proteins and plays a key role in the response to DNA damage (PubMed:16924674, PubMed:20064938, PubMed:21211721, PubMed:21270334, PubMed:25043379, PubMed:24598253).
Mediates mono-ADP-ribosylation of glutamate, aspartate or lysine residues on target proteins (PubMed:20064938, PubMed:25043379).
In contrast to PARP1 and PARP2, it is not able to mediate poly-ADP-ribosylation (PubMed:25043379).
Associates with a number of DNA repair factors and is involved in the response to exogenous and endogenous DNA strand breaks (PubMed:16924674, PubMed:21211721, PubMed:21270334).
Together with APLF, promotes the retention of the LIG4-XRCC4 complex on chromatin and accelerate DNA ligation during non-homologous end-joining (NHEJ) (PubMed:21211721).
Cooperates with the XRCC5-XRCC6 (Ku80-Ku70) heterodimer to limit end-resection thereby promoting accurate NHEJ (PubMed:24598253).
Involved in DNA repair by mediating mono-ADP-ribosylation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism, such as XRCC5 and XRCC6 (PubMed:16924674, PubMed:24598253).
ADP-ribosylation follows DNA damage and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks (PubMed:16924674, PubMed:21211721, PubMed:21270334).
May link the DNA damage surveillance network to the mitotic fidelity checkpoint (PubMed:16924674).
In addition to proteins, also able to ADP-ribosylate DNA: mediates DNA mono-ADP-ribosylation of DNA strand break termini via covalent addition of a single ADP-ribose moiety to a 5'- or 3'-terminal phosphate residues in DNA containing multiple strand breaks (PubMed:29361132, PubMed:29520010).
Acts as a negative regulator of immunoglobulin class switch recombination, probably by controlling the level of AICDA /AID on the chromatin (By similarity).

DNA ADP-ribosylationManual Assertion Based On ExperimentIDA:UniProtKB
DNA repairManual Assertion Based On ExperimentTAS:ProtInc
Double-strand break repairManual Assertion Based On ExperimentIDA:MGI
Negative regulation of isotype switchingISS:UniProtKB
Negative regulation of telomerase RNA reverse transcriptase activityManual Assertion Based On ExperimentIMP:BHF-UCL
Positive regulation of DNA ligationManual Assertion Based On ExperimentIGI:MGI
Positive regulation of double-strand break repair via nonhomologous end joiningManual Assertion Based On ExperimentIDA:UniProtKB
Protein ADP-ribosylationManual Assertion Based On ExperimentIDA:UniProtKB
Protein auto-ADP-ribosylationManual Assertion Based On ExperimentIDA:UniProtKB
Protein localization to site of double-strand breakManual Assertion Based On ExperimentIMP:MGI
Protein mono-ADP-ribosylationManual Assertion Based On ExperimentIDA:UniProtKB
Protein poly-ADP-ribosylationManual Assertion Based On ExperimentIBA:GO_Central
Regulation of mitotic spindle organizationManual Assertion Based On ExperimentIMP:MGI
Telomere maintenanceManual Assertion Based On ExperimentIMP:MGI

Nucleus
Chromosome
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole
Almost exclusively localized in the nucleus and appears in numerous small foci and a small number of larger foci whereas a centrosomal location has not been detected (PubMed:16924674).
In response to DNA damage, localizes to sites of double-strand break (PubMed:21270334).
Preferentially localized to the daughter centriole (PubMed:10329013).

Auto-mono-ADP-ribosylated.