PPME1

PPME1 is a protein phosphatase methylesterase localized to the nucleus. The encoded protein acts on the protein phosphatase-2A catalytic subunit and supports the ERK pathway through dephosphorylation of regulatory proteins. It plays a role in malignant glioma progression.

Full Name

Protein Phosphatase Methylesterase 1

Function

Demethylates proteins that have been reversibly carboxymethylated. Demethylates PPP2CB (in vitro) and PPP2CA. Binding to PPP2CA displaces the manganese ion and inactivates the enzyme.

Biological Process

G2/M transition of mitotic cell cycleTAS:Reactome
Protein demethylationManual Assertion Based On ExperimentIDA:HGNC-UCL
Protein phosphorylationIEA:Ensembl

Cellular Location

Nucleoplasm

PTM

Phosphorylated by SIK1 following increases in intracellular sodium, leading to dissociation from the protein phosphatase 2A (PP2A) complex and subsequent dephosphorylation of sodium/potassium-transporting ATPase ATP1A1.

Anti-PPME1 antibodies

Mouse Anti-PPME1 Recombinant Antibody (29G3) (CBMAB-P2573-YC)

Datasheet

SDS

Target: PPME1

Host: Mouse

Antibody Isotype: IgG1, κ

Specificity: Human

Clone: 29G3

Application*: WB, E, IC, IF

Mouse Anti-PPME1 Recombinant Antibody (10B2) (CBMAB-P2572-YC)

Datasheet

SDS

Target: PPME1

Host: Mouse

Antibody Isotype: IgG1

Specificity: Human

Clone: 10B2

Application*: F, IC, IF, WB

Mouse Anti-PPME1 Recombinant Antibody (10A12) (CBMAB-P2571-YC)

Datasheet

SDS

Target: PPME1

Host: Mouse

Antibody Isotype: IgG2a

Specificity: Human

Clone: 10A12

Application*: F, IC, IF, IH, P, WB

For Research Use Only. Not For Clinical Use.

(P): Predicted

* Abbreviations

IFImmunofluorescence

IHImmunohistochemistry

IPImmunoprecipitation

WBWestern Blot

EELISA

MMicroarray

CIChromatin Immunoprecipitation

FFlow Cytometry

FNFunction Assay

IDImmunodiffusion

RRadioimmunoassay

TCTissue Culture

GSGel Supershift

NNeutralization

BBlocking

AActivation

IInhibition

DDepletion

ESELISpot

DBDot Blot

MCMass Cytometry/CyTOF

CTCytotoxicity

SStimulation

AGAgonist

APApoptosis

IMImmunomicroscopy

BABioassay

CSCostimulation

EMElectron Microscopy

IEImmunoelectrophoresis

PAPeptide Array

ICImmunocytochemistry

PEPeptide ELISA

MDMeDIP

SHIn situ hybridization

IAEnzyme Immunoassay

SEsandwich ELISA

PLProximity Ligation Assay

ECELISA(Cap)

EDELISA(Det)

BIBioimaging

IOImmunoassay

LFLateral Flow Immunoassay

LALuminex Assay

CImmunohistochemistry-Frozen Sections

PImmunohistologyp-Paraffin Sections

ISIntracellular Staining for Flow Cytometry

MSElectrophoretic Mobility Shift Assay

RIRNA Binding Protein Immunoprecipitation (RIP)