TUBB3 Antibodies

Structure of TUBB3

The molecular weight of βIII -tubulin encoded by the TUBB3 gene is approximately 50 kDa, and this molecular weight fluctuates slightly among different mammalian species due to minor differences in amino acid sequences.

This protein is composed of 450 amino acids, and its three-dimensional structure contains a core GTP-binding domain (amino acids at positions 140-320) and an interaction interface that mediates tubulin dimerization. The arginine residue at position 262 constitutes the active center of GTPase, while the EEG motif at the C-terminal (amino acids at positions 430-450) regulates microtubule stability through electrostatic interactions with microtubule-associated proteins. These structural features jointly determine the specific function of TUBB3 in neurons.

Fig. 1 Schematic diagram of deleterious variants in TUBB3 functional domains.¹

Key structural properties of TUBB3:

• The core domain has GTP binding and hydrolysis
• The negatively charged C-terminal tail interacts with microtubule-associated proteins
• The arginine residue at position 262 is crucial for the activity of GTPase

Functions of TUBB3

The β III-tubulin encoded by TUBB3 is mainly involved in the construction and regulation of the neuronal microtubule system. Its specific functions include:

Compared with the widely expressed β I-tubulin, β III-tubulin endows microtubules with higher dynamic instability and stronger anti-depolymerization ability. This characteristic makes it particularly suitable for the development and repair processes that require highly plastic neural structures.

Applications of TUBB3 and TUBB3 Antibody in Literature

1. Puri, Dharmendra, Brenda J. Barry, and Elizabeth C. Engle. "TUBB3 and KIF21A in neurodevelopment and disease." Frontiers in Neuroscience 17 (2023): 1226181.https://doi.org/10.3389/fnins.2023.1226181

The article indicates that mutations in neuron-specific β -tubulin TUBB3 or kinesin KIF21A genes can disrupt microtubule dynamics and interactions, leading to abnormal neuronal migration or axonal growth orientation and triggering related neurological diseases.

2. Jin, Soohwa, et al. "TUBB3 M323V syndrome presents with infantile nystagmus." Genes 12.4 (2021): 575. https://doi.org/10.3390/genes12040575

The article indicates that the M323V variation of the TUBB3 gene can only present as nystagmus in infants. Although there are no other neurological symptoms, MRI reveals cortical dysplasia. The protein model shows that it interferes with the formation of TUBA1A dimer, suggesting that tubulinopathy should be investigated for isolated ocular fibrillation.

3. Sekino, Yohei, et al. "TUBB3 reverses resistance to docetaxel and cabazitaxel in prostate cancer." International journal of molecular sciences 20.16 (2019): 3936. https://doi.org/10.3390/ijms20163936

The article indicates that in prostate cancer, overexpression of TUBB3 can lead to resistance and cross-resistance to docetaxel and cabattaxel. The mechanism is related to the PTEN/PI3K pathway. The use of PI3K inhibitors can reduce the expression of TUBB3 and restore chemotherapy sensitivity.

4. Shao, Qiangqiang, et al. "Disease-associated mutations in human TUBB3 disturb netrin repulsive signaling." PLoS One 14.6 (2019): e0218811. https://doi.org/10.1371/journal.pone.0218811

Research has found that mutations in the human TUBB3 gene specifically disrupt the netrin-1/ UNC5C-mediated axon rejection signal, leading to growth cone collapse and abnormal axon orientation, thereby clarifying the molecular mechanism of related neurological diseases.

5. Radwitz, Jennifer, et al. "Tubb3 expression levels are sensitive to neuronal activity changes and determine microtubule growth and kinesin-mediated transport." Cellular and Molecular Life Sciences 79.11 (2022): 575. https://doi.org/10.1007/s00018-022-04607-5

Research has found that neuronal activity can regulate the expression of specific β -tubulin TUBB3. Its down-regulation will accelerate microtubule growth and change the motility of kinesin, thereby promoting synaptic cargo transport and revealing a new activity-dependent feedback mechanism.

Creative Biolabs: TUBB3 Antibodies for Research

Creative Biolabs specializes in the production of high-quality TUBB3 antibodies for research and industrial applications. Our portfolio includes monoclonal antibodies tailored for ELISA, Flow Cytometry, Western blot, immunohistochemistry, and other diagnostic methodologies.

• Custom TUBB3 Antibody Development: Tailor-made solutions to meet specific research requirements.
• Bulk Production: Large-scale antibody manufacturing for industry partners.
• Technical Support: Expert consultation for protocol optimization and troubleshooting.
• Aliquoting Services: Conveniently sized aliquots for long-term storage and consistent experimental outcomes.

For more details on our TUBB3 antibodies, custom preparations, or technical support, contact us at email.