ZNF2 Antibodies

Structure of ZNF2

Myoglobin is a relatively small protein with a molecular weight of approximately 16.7 kDa. This weight may slightly vary between species due to minor differences in amino acid sequence.

The protein encoded by the ZNF2 gene specifically recognizes DNA sequences through its tandem C2H2-type zinc finger module. Each zinc finger unit forms a stable finger-like structure by coordinating with zinc ions through conserved cysteine and histidine residues. This structure enables the protein to precisely bind to the GC-rich sequence in the promoter region of the target gene, thereby recruiting transcriptional inhibitory complexes such as histone deacetylase and achieving negative regulation of gene expression.

Fig. 1 The diagram of the RZE1-ZNF2 genomic region in XL280.¹

Key structural properties of ZNF2:

• Typical C2H2 type zinc finger array structure
• Each zinc finger coordinates with the zinc ion through conserved cysteine and histidine residues
• Basic amino acid residues constitute the nuclear localization signal domain
• Carboxyl terminal is rich in proline transcription suppress structural domain

Functions of ZNF2

The main function of the protein encoded by the ZNF2 gene is to act as a transcriptional suppressor to participate in the regulation of gene expression. At the same time, this protein is also involved in multiple biological processes, including the regulation of cell differentiation and the occurrence and development of tumors.

The ZNF2 protein binds to the GC-rich sequence with high affinity through its zinc finger domain. Each zinc finger module interacts with DNA in an independent and synergistic manner to form a specific transcriptional inhibitory complex. This binding mode enables it to precisely regulate the expression of a specific gene set.

Applications of ZNF2 and ZNF2 Antibody in Literature

1. Lin, Jianfeng, et al. "Immunoprotection against cryptococcosis offered by Znf2 depends on capsule and the hyphal morphology." MBio 13.1 (2022): e02785-21. https://doi.org/10.1128/mbio.02785-21

Previous studies have found that overexpression of ZNF2 can drive the filamentous growth of Cryptococcus and reduce its toxicity, and can also stimulate protective immune responses. Further research indicates that the capsular antigen content of ZNF2-overexpressing strains is higher, which can induce effective immune protection, and this process depends on the chromatin remodeling factor Brf1. The research suggests the immune potential of protective antigens on the surface of Cryptococcus.

2. Pham, Tuyetnhu, et al. "Vaccination with a ZNF2 Oe strain of Cryptococcus provides long-lasting protection against cryptococcosis and is effective in immunocompromised hosts." Infection and Immunity 91.7 (2023): e00198-23. https://doi.org/10.1128/iai.00198-23

Research has found that Cryptococcus (live or inactivated) with overexpression of ZNF2 can be used as a vaccine to induce long-lasting immune protection. Its efficacy is not dependent on CD4+ T cells and remains effective even in immune-deficient hosts, providing a new strategy for the development of broad-spectrum anti-cryptococcus vaccines for the population.

3. Lin, Jianfeng, et al. "Transcription factor Znf2 coordinates with the chromatin remodeling SWI/SNF complex to regulate cryptococcal cellular differentiation." Communications biology 2.1 (2019): 412. https://doi.org/10.1038/s42003-019-0665-2

The article indicates that in Cryptococcus, the transcription factor Znf2 regulates yeast-mycelial differentiation. Research has found that Brf1, together with Snf5 and others, form the SWI/SNF chromatin remodeling complex, which collaborates with Znf2 to open the promoter region of the target gene and promote its binding to the target site, revealing the unique role of this complex in morphogenesis in basidiomycetes.

4. Lin, Xiaorong, et al. "Transcription factors Mat2 and Znf2 operate cellular circuits orchestrating opposite-and same-sex mating in Cryptococcus neoformans." PLoS genetics 6.5 (2010): e1000953. https://doi.org/10.1371/journal.pgen.1000953

Research has found that the zinc finger protein transcription factor Znf2 is a key terminal regulatory factor for the mycelial morphological development of Cryptococcus neoformococcus, and it is regulated by Mat2 downstream of the MAPK pathway. Znf2 is not dependent on mating type sites and can negatively regulate virulence. It is a core factor connecting fungal development and pathogenicity.

5. Chacko, Nadia, et al. "The lncRNA RZE1 controls cryptococcal morphological transition." PLoS genetics 11.11 (2015): e1005692. https://doi.org/10.1371/journal.pgen.1005692

Research has found that the long non-coding RNA RZE1 controls the yea-mycelial morphological transformation of Cryptococcus neoformococcus by regulating the expression of the transcription factor Znf2. RZE1 mainly promotes the cytoplasmic output and transcriptional activity of ZNF2 mRNA in the cell nucleus through cis-acting, thereby influencing the expression of its downstream target genes. This discovery reveals a novel regulatory mechanism of lncRNA in the morphological development of fungi.

Creative Biolabs: ZNF2 Antibodies for Research

Creative Biolabs specializes in the production of high-quality ZNF2 antibodies for research and industrial applications. Our portfolio includes monoclonal antibodies tailored for ELISA, Flow Cytometry, Western blot, immunohistochemistry, and other diagnostic methodologies.

• Custom ZNF2 Antibody Development: Tailor-made solutions to meet specific research requirements.
• Bulk Production: Large-scale antibody manufacturing for industry partners.
• Technical Support: Expert consultation for protocol optimization and troubleshooting.
• Aliquoting Services: Conveniently sized aliquots for long-term storage and consistent experimental outcomes.

For more details on our ZNF2 antibodies, custom preparations, or technical support, contact us at email.