Human MYH2 ELISA Kit (V2LY-0626-LY4921)

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Tested Data
Datasheet
SDS
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Basic Information

Sensitivity
0.054 ng/mL
Detection Range
0.1-40 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
223.0 kDa
Components
  • Pre-coated ELISA plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
MYH2
Function
Muscle contraction. Required for cytoskeleton organization (By similarity).
Biological Process
Muscle contraction Source: BHF-UCL
Muscle filament sliding Source: BHF-UCL
Cellular Location
Other locations
myofibril
Note: Thick filaments of the myofibrils.
Involvement in disease
Myopathy, proximal, with ophthalmoplegia (MYPOP):
A muscular disorder characterized by mild-to-moderate muscle weakness, ophthalmoplegia, and contractures at birth in some patients. Muscle biopsies from patients show predominance of type 1 fibers and small or absent type 2A fibers. The disease is non-progressive or it progresses very slowly. Inheritance is autosomal dominant or recessive.

Jo, S., Lee, S. H., Jeon, C., Jo, H. R., You, Y. J., Lee, J. K., ... & Lee, C. H. (2023). Myosin heavy chain 2 (MYH2) expression in hypertrophic chondrocytes of soft callus provokes endochondral bone formation in fracture. Life Sciences, 334, 122204.

Sonne, A., Peverelli, L., Hernandez-Lain, A., Domínguez-González, C., Andersen, J. L., Milone, M., ... & Ochala, J. (2023). Myosin post-translational modifications and function in the presence of myopathy-linked truncating MYH2 mutations. American Journal of Physiology-Cell Physiology, 324(3), C769-C776.

Hitachi, K., Kiyofuji, Y., Yamaguchi, H., Nakatani, M., Inui, M., & Tsuchida, K. (2023). Simultaneous loss of skeletal muscle myosin heavy chain IIx and IIb causes severe skeletal muscle hypoplasia in postnatal mice. The FASEB Journal, 37(1), e22692.

Hedberg-Oldfors, C., Elíasdóttir, Ó., Geijer, M., Lindberg, C., & Oldfors, A. (2022). Dominantly inherited myosin IIa myopathy caused by aberrant splicing of MYH2. BMC neurology, 22(1), 1-9.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Pig MYH2 ELISA Kit (CAT#: V2LY-0626-LY993)

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Mouse Anti-MYH2 Recombinant Antibody (A4.1519) (CAT#: CBMAB-0334-CN)

Mouse Anti-MYH2 Antibody (BF-35) (CAT#: CBMAB-1438CQ)

Mouse Anti-MYH2 Recombinant Antibody (CBFYM-0438) (CAT#: CBMAB-M0553-FY)

Pig MYH2 ELISA Kit (CAT#: V2LY-0626-LY993)

Mouse Anti-MYH2 Antibody (BF-32) (CAT#: CBMAB-1437CQ)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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