LRP1 Antibodies

Structure of LRP1

LRP1 is a large type I transmembrane protein with a molecular weight of approximately 515 kDa. This protein is composed of an extracellular α chain of approximately 500 kDa and a transmembrane β chain of 85 kDa linked by non-covalent bonds. Its molecular weight fluctuates slightly among different species due to differences in glycosylation modifications.

The extracellular segment of LRP1 is composed of four ligand binding clusters (I-IV), including 31 EGF-like repeat sequences and 22 typical LDLR domains, forming multiple ligand binding sites. Its transmembrane region is a single-pass transmembrane structure, and the intracellular segment contains two NPxY internalizing moieties, which are responsible for mediating mesin-dependent endocytosis processes and cellular signal transduction. This protein specifically binds to over 40 ligands, including apolipoprotein E and α 2-macroglobulin, through its modular extracellular domain, playing a core role in lipid metabolism and cellular signal regulation.

Fig. 1 Schematic diagram of domain structure for LRP1 and LDLR.¹

Key structural properties of LRP1:

• Giant modular extracellular domain (containing 4 ligand binding clusters)
• Highly conservative transmembrane helical anchoring structure
• Intracellular segment double NPxY internalized motifs mediate signal transduction
• Multiple EGF-like repeat sequences regulate PH-dependent ligand release

Functions of LRP1

The main function of the LRP1 gene is to act as a multifunctional receptor on the cell surface, mediating ligand-localization and cell signal transduction. However, it is also widely involved in various physiological and pathological processes, including lipid metabolism, cell migration and tissue remodeling.

The binding affinity of LRP1 to ligands is synergistic regulated by its multiple helper receptors, which contrasts sharply with the single oxygen-binding mode of myoglobin and highlights its core position as a complex signal integration center.

Applications of LRP1 and LRP1 Antibody in Literature

1. Shinohara, Mitsuru, et al. "Role of LRP1 in the pathogenesis of Alzheimer's disease: evidence from clinical and preclinical studies: Thematic Review Series: ApoE and Lipid Homeostasis in Alzheimer's Disease." Journal of lipid research 58.7 (2017): 1267-1281. https://doi.org/10.1194/jlr.R075796

In the research of Alzheimer's disease (AD), LRP1, as A member of the low-density lipoprotein receptor family, participates in the disease process in both Aβ -dependent and non-dependent pathways by regulating Aβ metabolism, maintaining brain homeostasis and influencing apoE action. Both clinical and preclinical studies support its potential pathogenic mechanism, but its specific role still needs in-depth exploration, and its value as a therapeutic target has also attracted much attention.

2. Chen, Kai, et al. "LRP1 is a neuronal receptor for α-synuclein uptake and spread." Molecular neurodegeneration 17.1 (2022): 57. https://doi.org/10.1186/s13024-022-00560-w

This study confirmed that LRP1 is a key receptor regulating the uptake of α -Syn by neurons and the transmission of α -SYN in the brain. Gene knockout experiments have shown that the deletion of LRP1 significantly reduces the uptake of α -Syn monomers, oligomers and pathological fibers by neurons, and effectively inhibits the spread of α -Syn in mouse models. This discovery reveals the core role of LRP1 in synucleinopathies such as Parkinson's disease, providing a new target for treatment.

3. Yan, Wenjin, et al. "Heterozygous LRP1 deficiency causes developmental dysplasia of the hip by impairing triradiate chondrocytes differentiation due to inhibition of autophagy." Proceedings of the National Academy of Sciences 119.37 (2022): e2203557119. https://doi.org/10.1073/pnas.2203557119

This study has for the first time discovered that pathogenic variations in the LRP1 gene are closely related to congenital hip dysplasia (DDH). Animal models have confirmed that the absence of LRP1 accelerates the premature development of tridirectional cartilage, leading to acetabular and femoral head deformities, and significantly weakens the ability of cartilage formation. This study reveals the key role of LRP1 in the etiology of DDH, providing a new direction for treatment.

4. He, Lingnan, et al. "FUT2 inhibits the EMT and metastasis of colorectal cancer by increasing LRP1 fucosylation." Cell Communication and Signaling 21.1 (2023): 63. https://doi.org/10.1186/s12964-023-01060-0

This study reveals that the FUT2 enzyme inhibits the epithelial-mesenchymal transition (EMT) and metastasis process of colorectal cancer by mediating α-1,2 fucosylation modification of the LRP1 protein. Experiments have shown that the absence of FUT2 weakens the tumor suppressor function of LRP1, promoting tumor invasion and spread. This discovery clarifies the key role of LRP1 in the FUT2 tumor suppressor pathway and provides a new target for treatment.

5. Liu, Lu, et al. "LRP1 Repression by SNAIL Results in ECM Remodeling in Genetic Risk for Vascular Diseases." Circulation Research 135.11 (2024): 1084-1097. https://doi.org/10.1161/CIRCRESAHA.124.325269

This study reveals that the pathogenic variant rs11172113 located in the LRP1 gene regulates its expression through an enhancer region. The transcription factor SNAIL can specifically bind to this risk allele, thereby inhibiting the expression of LRP1. The deletion of LRP1 alters the extracellular matrix of smooth muscle cells and enhances the TGF-β signaling pathway, which provides a potential mechanism for explaining why the LRP1 locus is a common risk factor for various vascular diseases.

Creative Biolabs: LRP1 Antibodies for Research

Creative Biolabs specializes in the production of high-quality LRP1 antibodies for research and industrial applications. Our portfolio includes monoclonal antibodies tailored for ELISA, Flow Cytometry, Western blot, immunohistochemistry, and other diagnostic methodologies.

• Custom LRP1 Antibody Development: Tailor-made solutions to meet specific research requirements.
• Bulk Production: Large-scale antibody manufacturing for industry partners.
• Technical Support: Expert consultation for protocol optimization and troubleshooting.
• Aliquoting Services: Conveniently sized aliquots for long-term storage and consistent experimental outcomes.

For more details on our LRP1 antibodies, custom preparations, or technical support, contact us at email.