MYL12B

The activity of nonmuscle myosin II is regulated by phosphorylation of a regulatory light chain, such as MRLC2. This phosphorylation results in higher MgATPase activity and the assembly of myosin II filaments.

Full Name

MYOSIN LIGHT CHAIN 12B

Function

Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Phosphorylation triggers actin polymerization in vascular smooth muscle. Implicated in cytokinesis, receptor capping, and cell locomotion.

Biological Process

Regulation of cell shape Source: Ensembl

Cellular Location

Cytoskeleton
myosin II complex
stress fiber
Cytosol
extracellular exosome
Other locations
apical part of cell
brush border
cell cortex
cytoplasm
myofibril
Z disc

PTM

Phosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge. Phosphorylation is reduced following epigallocatechin-3-O-gallate treatment.

Anti-MYL12B antibodies

Mouse Anti-MYL12B Recombinant Antibody (CBFYM-2950) (CBMAB-M3145-FY)

Datasheet

SDS

Target: MYL12B

Host: Mouse

Antibody Isotype: IgG1

Specificity: Human

Clone: CBFYM-2950

Application*: E, WB

Mouse Anti-MYL12B Recombinant Antibody (CBFYM-0720) (CBMAB-M0862-FY)

Datasheet

SDS

Target: MYL12B

Host: Mouse

Antibody Isotype: IgG1, κ

Specificity: Human

Clone: CBFYM-0720

Application*: E

Mouse Anti-MYL12B Recombinant Antibody (1B22) (CBMAB-A5580-LY)

Datasheet

SDS

Target: MYL12B

Host: Mouse

Antibody Isotype: IgG1, κ

Specificity: Human

Clone: 1B22

Application*: SE, E

For Research Use Only. Not For Clinical Use.

(P): Predicted

* Abbreviations

IFImmunofluorescence

IHImmunohistochemistry

IPImmunoprecipitation

WBWestern Blot

EELISA

MMicroarray

CIChromatin Immunoprecipitation

FFlow Cytometry

FNFunction Assay

IDImmunodiffusion

RRadioimmunoassay

TCTissue Culture

GSGel Supershift

NNeutralization

BBlocking

AActivation

IInhibition

DDepletion

ESELISpot

DBDot Blot

MCMass Cytometry/CyTOF

CTCytotoxicity

SStimulation

AGAgonist

APApoptosis

IMImmunomicroscopy

BABioassay

CSCostimulation

EMElectron Microscopy

IEImmunoelectrophoresis

PAPeptide Array

ICImmunocytochemistry

PEPeptide ELISA

MDMeDIP

SHIn situ hybridization

IAEnzyme Immunoassay

SEsandwich ELISA

PLProximity Ligation Assay

ECELISA(Cap)

EDELISA(Det)

BIBioimaging

IOImmunoassay

LFLateral Flow Immunoassay

LALuminex Assay

CImmunohistochemistry-Frozen Sections

PImmunohistologyp-Paraffin Sections

ISIntracellular Staining for Flow Cytometry

MSElectrophoretic Mobility Shift Assay

RIRNA Binding Protein Immunoprecipitation (RIP)