POLN

Broad expression in testis adult (RPKM 3.7), CNS E11.5 (RPKM 2.7) and 18 other tissues.

Full Name

DNA Polymerase N

Function

DNA polymerase with very low fidelity that catalyzes considerable misincorporation by inserting dTTP opposite a G template, and dGTP opposite a T template (PubMed:16787914, PubMed:17118716).
Is the least accurate of the DNA polymerase A family (i.e. POLG, POLN and POLQ) (PubMed:17118716).
Can perform accurate translesion DNA synthesis (TLS) past a 5S-thymine glycol. Can perform efficient strand displacement past a nick or a gap and gives rise to an amount of product similar to that on non-damaged template. Has no exonuclease activity (PubMed:16787914).
Error-prone DNA polymerase that preferentially misincorporates dT regardless of template sequence (PubMed:25775266).
May play a role in TLS during interstrand cross-link (ICL) repair (PubMed:19908865).
May be involved in TLS when genomic replication is blocked by extremely large major groove DNA lesions. May function in the bypass of some DNA-protein and DNA-DNA cross-links. May have a role in cellular tolerance to DNA cross-linking agents (PubMed:20102227).
Involved in the repair of DNA cross-links and double-strand break (DSB) resistance. Participates in FANCD2-mediated repair. Forms a complex with HELQ helicase that participates in homologous recombination (HR) repair and is essential for cellular protection against DNA cross-links (PubMed:19995904).

Biological Process

DNA-templated DNA replicationIEA:InterPro
Double-strand break repairManual Assertion Based On ExperimentIBA:GO_Central
Double-strand break repair via homologous recombinationManual Assertion Based On ExperimentIMP:UniProtKB
Interstrand cross-link repairManual Assertion Based On ExperimentIMP:UniProtKB
Translesion synthesisManual Assertion Based On ExperimentIDA:UniProtKB

Cellular Location

Nucleus