SOX2 Antibodies

Structure of SOX2

The molecular weight of the protein encoded by the SOX2 gene is approximately 34 kDa. This value varies slightly among different species, mainly due to differences in amino acid composition and post-translational modifications.

The SOX2 protein is composed of 317 amino acids and belongs to higher-order transcription factors, featuring a typical DNA-binding HMG domain. This domain is composed of three α -helices, which can bend DNA and promote its specific binding to the target sequence. The N-terminal of the protein is rich in basic amino acids and is responsible for nuclear localization. The C-terminal contains a transcriptional activation region, and most species have conserved proline and serine residues. The arginine and asparagine residues in the HMG box play a crucial role in DNA recognition and binding affinity.

Fig. 1 SOX2/Sox2 is a molecular target of various secondary modifications.¹

Key structural properties of SOX2:

• Conserved HMG-box domains mediate DNA bending and binding
• The C transcription activation area rich in proline/serine
• Check and ratify a signal in n-terminal protein

Functions of SOX2

The core function of the SOX2 gene is to maintain cellular pluripotency and regulate the early development process. In addition, it is also involved in neural differentiation, retinal formation and the occurrence mechanisms of various diseases.

SOX2 regulates the gene expression network in a synergistic or inhibitory manner by binding to the conserved sequence in the enhancer region of the target gene. Its function is highly background dependent and exerts pleiotropic effects in different cell types and developmental stages.

Applications of SOX2 and SOX2 Antibody in Literature

1. Mercurio, Sara, et al. "Deconstructing Sox2 function in brain development and disease." Cells 11.10 (2022): 1604. https://doi.org/10.3390/cells11101604

The article indicates that SOX2 is a key transcription factor regulating the development of the central nervous system. Its absence can lead to specific abnormalities in multiple brain regions such as the hippocampus and cortex in mice and humans, with obvious spatiotemporal and cell type dependence.

2. Zhu, Yin, et al. "SOX2 promotes chemoresistance, cancer stem cells properties, and epithelial–mesenchymal transition by β-catenin and Beclin1/autophagy signaling in colorectal cancer." Cell death & disease 12.5 (2021): 449. https://doi.org/10.1038/s41419-021-03733-5

The article indicates that SOX2 promotes the expression of ABCC2 by activating the β-catenin/ Beclin1-autophagy signaling axis, thereby driving chemotherapy resistance, dry maintenance and EMT process in colorectal cancer. Its high expression is associated with poor prognosis in patients.

3. Mercurio, Sara. "SOX2-Sensing: Insights into the Role of SOX2 in the Generation of Sensory Cell Types in Vertebrates." International Journal of Molecular Sciences 24.8 (2023): 7637. https://doi.org/10.3390/ijms24087637

The article indicates that SOX2 is a key regulatory factor in the development of the nervous system and the formation of sensory organs. Its mutation can cause various defects and participate in the differentiation of multiple sensory cells by maintaining neural stem cells.

4. Schaefer, Thorsten, and Claudia Lengerke. "SOX2 protein biochemistry in stemness, reprogramming, and cancer: the PI3K/AKT/SOX2 axis and beyond." Oncogene 39.2 (2020): 278-292. https://doi.org/10.1038/s41388-019-0997-x

The article indicates that the activity of SOX2 is precisely regulated by multiple protein modifications such as phosphorylation and methylation. These modifications interact with the PI3K/AKT pathway and play a key role in pluripotency, reprogramming, and carcinogenesis.

5. Lin, Zhi-Bo, et al. "Novel SOX2 mutation in autosomal dominant cataract-microcornea syndrome." BMC ophthalmology 22.1 (2022): 70. https://doi.org/10.1186/s12886-022-02291-4

In a Chinese Han family with congenital cataract and microcorneal syndrome (CCMC), this study identified a novel missense mutation, c.295G>T (p.al99SER), in the SOX2 gene. This mutation is located in the highly conserved HMG domain and may affect protein function, expanding the mutation spectrum of SOX2 and the understanding of the etiology of CCMC.

Creative Biolabs: SOX2 Antibodies for Research

Creative Biolabs specializes in the production of high-quality SOX2 antibodies for research and industrial applications. Our portfolio includes monoclonal antibodies tailored for ELISA, Flow Cytometry, Western blot, immunohistochemistry, and other diagnostic methodologies.

• Custom SOX2 Antibody Development: Tailor-made solutions to meet specific research requirements.
• Bulk Production: Large-scale antibody manufacturing for industry partners.
• Technical Support: Expert consultation for protocol optimization and troubleshooting.
• Aliquoting Services: Conveniently sized aliquots for long-term storage and consistent experimental outcomes.

For more details on our SOX2 antibodies, custom preparations, or technical support, contact us at email.