Can you provide a recommended protocol for Immunohistochemistry (IHC)?

For IHC on FFPE tissue sections, we recommend deparaffinization followed by heat-induced epitope retrieval (HIER) using a 10 mM Sodium Citrate buffer (pH 6.0) for 15-20 minutes. After blocking, the primary antibody can be incubated at a starting dilution of 1:100 to 1:200 overnight at 4°C. However, optimal conditions, including incubation times and antigen retrieval methods, should be determined empirically by the end-user for their specific tissue type and protocol.

How was the specificity of this ACVR1 antibody validated?

This antibody's specificity was rigorously validated by Western Blotting using human cell lysates known to express ACVR1, where it detected a single band at the correct molecular weight. The recombinant nature of the antibody, being derived from a single defined clone, inherently ensures high specificity to its target epitope compared to traditional polyclonal antibodies.

Mouse Anti-ACVR1 Recombinant Antibody (V2-179666) (CBMAB-A1022-YC)

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Datasheet

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Basic Information

Host Animal

Mouse

Clone

V2-179666

Application

ELISA, WB

Immunogen

ACVR1 (AAH33867, 21 a.a. ~ 120 a.a) partial recombinant protein with GST tag.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

PBS, pH 7.4

Buffer

PBS, pH7.4

Preservative

None

Concentration

Batch dependent

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

activin A receptor, type I

Introduction

Activins are dimeric growth and differentiation factors which belong to the transforming growth factor-beta (TGF-beta) superfamily of structurally related signaling proteins. Activins signal through a heteromeric complex of receptor serine kinases which i

Entrez Gene ID

UniProt ID

Q04771

Alternative Names

Activin A Receptor Type 1; Serine/Threonine-Protein Kinase Receptor R1; TGF-B Superfamily Receptor Type I; Activin Receptor-Like Kinase 2; Activin A Receptor, Type I; Activin Receptor Type I; EC 2.7.11.30; ACVRLK2; SKR1; Activin A Receptor, Type II-Like K

Function

On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for activin. May be involved for left-right pattern formation during embryogenesis (By similarity).

Biological Process

Activin receptor signaling pathway
Acute inflammatory response
Angiogenesis
Atrial septum primum morphogenesis
Atrioventricular valve morphogenesis
BMP signaling pathway
BMP signaling pathway involved in heart development
Branching involved in blood vessel morphogenesis
Cardiac muscle cell fate commitment
Cellular response to BMP stimulus
Cellular response to growth factor stimulus
Determination of left/right symmetry
Dorsal/ventral pattern formation
Embryonic heart tube morphogenesis
Endocardial cushion cell fate commitment
Endocardial cushion fusion
Endocardial cushion morphogenesis
G1/S transition of mitotic cell cycle
Gastrulation with mouth forming second
Germ cell development
Heart development
In utero embryonic development
Mesoderm formation
Mitral valve morphogenesis
Negative regulation of activin receptor signaling pathway
Negative regulation of extrinsic apoptotic signaling pathway
Negative regulation of signal transduction
Neural crest cell migration
Pathway-restricted SMAD protein phosphorylation
Peptidyl-threonine phosphorylation
Pharyngeal system development
Positive regulation of bone mineralization
Positive regulation of cell migration
Positive regulation of determination of dorsal identity
Positive regulation of epithelial to mesenchymal transition involved in endocardial cushion formation
Positive regulation of osteoblast differentiation
Positive regulation of pathway-restricted SMAD protein phosphorylation
Positive regulation of peptidyl-tyrosine phosphorylation
Positive regulation of transcription, DNA-templated
Positive regulation of transcription by RNA polymerase II
Protein phosphorylation
Regulation of ossification
Smooth muscle cell differentiation
Transforming growth factor beta receptor signaling pathway
Ventricular septum morphogenesis

Cellular Location

Membrane

Involvement in disease

Fibrodysplasia ossificans progressiva (FOP): A rare autosomal dominant connective tissue disorder resulting in skeletal malformations and progressive extraskeletal ossification. Heterotopic ossification begins in childhood and can be induced by trauma or may occur without warning. Bone formation is episodic and progressive, leading to a debilitating ankylosis of all major joints of the axial and appendicular skeleton, rendering movement impossible.

Topology

Extracellular: 21-123 aa
Helical: 124-146 aa
Cytoplasmic: 147-509 aa

Katagiri, T., Tsukamoto, S., & Kuratani, M. (2021). Accumulated Knowledge of Activin Receptor-Like Kinase 2 (ALK2)/Activin A Receptor, Type 1 (ACVR1) as a Target for Human Disorders. Biomedicines, 9(7), 736.

Lin, J. B., Chen, J. F., Lai, F. C., Li, X., Xie, J. B., Tu, Y. R., & Kang, M. Q. (2020). Involvement of activin a receptor type 1 (ACVR1) in the pathogenesis of primary focal hyperhidrosis. Biochemical and biophysical research communications, 528(2), 299-304.

Aykul, S., Corpina, R. A., Goebel, E. J., Cunanan, C. J., Dimitriou, A., Kim, H. J., ... & Idone, V. (2020). Activin A forms a non-signaling complex with ACVR1 and type II Activin/BMP receptors via its finger 2 tip loop. Elife, 9, e54582.

Omi, M., Kaartinen, V., & Mishina, Y. (2019). Activin A receptor type 1–mediated BMP signaling regulates RANKL-induced osteoclastogenesis via canonical SMAD-signaling pathway. Journal of Biological Chemistry, 294(47), 17818-17836.

Valer, J. A., Sánchez-de-Diego, C., Pimenta-Lopes, C., Rosa, J. L., & Ventura, F. (2019). ACVR1 function in health and disease. Cells, 8(11), 1366.

Cheng, J., Cao, X., Hao, D., Ma, Y., Qi, X., Chaogetu, B., ... & Chen, H. (2019). The ACVR1 gene is significantly associated with growth traits in Chinese beef cattle. Livestock Science, 229, 210-215.

Wang, Y., Sun, J. C., Wang, H. B., Xu, X. M., Kong, Q. J., Wang, Y. J., ... & Shi, J. G. (2019). ACVR1‐knockout promotes osteogenic differentiation by activating the Wnt signaling pathway in mice. Journal of cellular biochemistry, 120(5), 8185-8194.

Haupt, J., Xu, M., & Shore, E. M. (2018). Variable signaling activity by FOP ACVR1 mutations. Bone, 109, 232-240.

Hildebrand, L., Stange, K., Deichsel, A., Gossen, M., & Seemann, P. (2017). The Fibrodysplasia Ossificans Progressiva (FOP) mutation p. R206H in ACVR1 confers an altered ligand response. Cellular signalling, 29, 23-30.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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