Rabbit Anti-AFDN Monoclonal Antibody (V2-5997) (CBMAB-1745-YC)

Name: Rabbit Anti-AFDN Monoclonal Antibody (V2-5997)
SKU: CBMAB-1745-YC
Rating: 5 (1 reviews)

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Published Data

Rabbit Anti-AFDN Monoclonal Antibody (A7L9H48) (CBMAB-1745-YC) in IP

IP of Cx36 from retina and brain, showing the presence of AF6 in IP material (lanes 3 and 4), with brain (lane 1) and retina (lane 5) homogenates included as positive controls for AF6 detection, and IP antibody omission as a negative control (lane 2). Note detection of both sAF6 and lAF6 after Cx36 IP (lanes 3 and 4). ...View More

Li, X., Lynn, B. D., & Nagy, J. I. (2012). The effector and scaffolding proteins AF6 and MUPP1 interact with connexin36 and localize at gap junctions that form electrical synapses in rodent brain. European Journal of Neuroscience, 35(2), 166-181.

Rabbit Anti-AFDN Monoclonal Antibody (A7L9H48) (CBMAB-1745-YC) in IF

Laser scanning confocal double immunofluorescence labelling for AF6 in combination with labelling for Cx36 and ZO-1 in various regions of adult mouse brain. (A, B) Double labelling for AF6 (A1, B1, arrows) and Cx36 (A2, B2, arrows) in the basomedial nucleus of the amygdala with two different anti-AF6 antibodies, one directed against the AF6 N terminus (A1-A3, showing the same field) and the other against the AF6 C terminus (B1-B3, showing the same field) Substantial AF6 ⁄ Cx36 co-localization is seen with both anti-AF6 antibodies (A3, B3, arrows). (G) The same field (C1-C3) in the IPL of retina double-labelled for AF6 ⁄ ZO-1, showing AF6-puncta (C1, arrows) co-localized with ZO-1-puncta (C2, arrows), as seen in overlay (C3, arrows). Scale bars: A-C, 10 lm. ...View More

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

V2-5997

Application

ICC, IF, WB

Immunogen

Synthetic peptide corresponding to Mouse L-afadin + S-afadin aa 1805-1820 (C terminal)

Host Species

Rabbit

Specificity

Mouse

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1-2 μg/ml
IHC-P	1-2 μg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

0.09% sodium azide

Concentration

0.5 mg/ml

Purity

>95%, as determined by SDS-PAGE analysis

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Afadin, Adherens Junction Formation Factor

Introduction

AFDN belongs to an adhesion system, probably together with the E-cadherin-catenin system, which plays a role in the organization of homotypic, interneuronal and heterotypic cell-cell adherens junctions (AJs). Nectin- and actin-filament-binding protein that connects nectin to the actin cytoskeleton.

Entrez Gene ID

>17356

UniProt ID

>Q9QZQ1

Alternative Names

AF6; Af-6; Gm314; Mllt4; Afadin; I-afadin; S-afadin; 5033403D15Rik

Function

Belongs to an adhesion system, probably together with the E-cadherin-catenin system, which plays a role in the organization of homotypic, interneuronal and heterotypic cell-cell adherens junctions (AJs) (By similarity). Nectin- and actin-filament-binding protein that connects nectin to the actin cytoskeleton. May play a key role in the organization of epithelial structures of the embryonic ectoderm (By similarity). Essential for the organization of adherens junctions.

Biological Process

Adherens junction organization
Bicellular tight junction assembly
Cell adhesion
Cell-cell adhesion mediated by cadherin
Cell-cell signaling
Establishment of endothelial intestinal barrier
Establishment of protein localization to plasma membrane
Negative regulation of cell migration
Pore complex assembly
Positive regulation of cell-cell adhesion
Positive regulation of cell-cell adhesion mediated by cadherin
Positive regulation of gene expression
Positive regulation of GTPase activity
Regulation of protein localization
Signal transduction

Cellular Location

Adherens junction. Not found at cell-matrix AJs.

Huret, J. L., & Dessen, P. (2019). AFDN (afadin, adherens junction formation factor). Atlas of Genetics and Cytogenetics in Oncology and Haematology.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Mouse Anti-AFDN Recombinant Antibody (V2-58751) (CAT#: CBMAB-L0408-YJ)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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