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Rabbit Anti-AFDN Monoclonal Antibody (V2-5997) (CBMAB-1745-YC)

Provided herein is a rabbit monoclonal antibody against Mouse AFDN. The antibody, clone A7L9H48, can be used for immunoassay techniques, such as ICC, IF and WB.
See all AFDN antibodies
Published Data
Rabbit Anti-AFDN Monoclonal Antibody (A7L9H48) (CBMAB-1745-YC) in IP
IP of Cx36 from retina and brain, showing the presence of AF6 in IP material (lanes 3 and 4), with brain (lane 1) and retina (lane 5) homogenates included as positive controls for AF6 detection, and IP antibody omission as a negative control (lane 2). Note detection of both sAF6 and lAF6 after Cx36 IP (lanes 3 and 4). ...View More
Li, X., Lynn, B. D., & Nagy, J. I. (2012). The effector and scaffolding proteins AF6 and MUPP1 interact with connexin36 and localize at gap junctions that form electrical synapses in rodent brain. European Journal of Neuroscience, 35(2), 166-181.
Rabbit Anti-AFDN Monoclonal Antibody (A7L9H48) (CBMAB-1745-YC) in IF
Laser scanning confocal double immunofluorescence labelling for AF6 in combination with labelling for Cx36 and ZO-1 in various regions of adult mouse brain. (A, B) Double labelling for AF6 (A1, B1, arrows) and Cx36 (A2, B2, arrows) in the basomedial nucleus of the amygdala with two different anti-AF6 antibodies, one directed against the AF6 N terminus (A1-A3, showing the same field) and the other against the AF6 C terminus (B1-B3, showing the same field) Substantial AF6 ⁄ Cx36 co-localization is seen with both anti-AF6 antibodies (A3, B3, arrows). (G) The same field (C1-C3) in the IPL of retina double-labelled for AF6 ⁄ ZO-1, showing AF6-puncta (C1, arrows) co-localized with ZO-1-puncta (C2, arrows), as seen in overlay (C3, arrows). Scale bars: A-C, 10 lm. ...View More
Li, X., Lynn, B. D., & Nagy, J. I. (2012). The effector and scaffolding proteins AF6 and MUPP1 interact with connexin36 and localize at gap junctions that form electrical synapses in rodent brain. European Journal of Neuroscience, 35(2), 166-181.

Summary

Host Animal
Rabbit
Specificity
Mouse
Clone
V2-5997
Antibody Isotype
IgG
Application
ICC, IF, WB

Basic Information

Immunogen
Synthetic peptide corresponding to Mouse l + S Afadin aa 1805-1820 (C terminal)
Host Species
Rabbit
Specificity
Mouse
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1-2 μg/ml
IHC-P1-2 μg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS
Preservative
0.09% sodium azide
Concentration
0.5 mg/ml
Purity
>95%, as determined by SDS-PAGE analysis
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Afadin, Adherens Junction Formation Factor
Introduction
AFDN belongs to an adhesion system, probably together with the E-cadherin-catenin system, which plays a role in the organization of homotypic, interneuronal and heterotypic cell-cell adherens junctions (AJs). Nectin- and actin-filament-binding protein that connects nectin to the actin cytoskeleton.
Entrez Gene ID
>17356
UniProt ID
>Q9QZQ1
Alternative Names
AF6; Af-6; Gm314; Mllt4; Afadin; I-afadin; S-afadin; 5033403D15Rik
Function
Belongs to an adhesion system, probably together with the E-cadherin-catenin system, which plays a role in the organization of homotypic, interneuronal and heterotypic cell-cell adherens junctions (AJs) (By similarity). Nectin- and actin-filament-binding protein that connects nectin to the actin cytoskeleton. May play a key role in the organization of epithelial structures of the embryonic ectoderm (By similarity). Essential for the organization of adherens junctions.
Biological Process
Adherens junction organization
Bicellular tight junction assembly
Cell adhesion
Cell-cell adhesion mediated by cadherin
Cell-cell signaling
Establishment of endothelial intestinal barrier
Establishment of protein localization to plasma membrane
Negative regulation of cell migration
Pore complex assembly
Positive regulation of cell-cell adhesion
Positive regulation of cell-cell adhesion mediated by cadherin
Positive regulation of gene expression
Positive regulation of GTPase activity
Regulation of protein localization
Signal transduction
Cellular Location
Adherens junction. Not found at cell-matrix AJs.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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