Mouse Anti-AKAP9 Recombinant Antibody (V2-12556) (CBMAB-1046-CN)

Name: Mouse Anti-AKAP9 Recombinant Antibody (V2-12556)
SKU: CBMAB-1046-CN
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-AKAP9 Recombinant Antibody (17G10) in IF

AKAP expression in hippocampal neurons.(a) Immunostaining for AKAP9 and Tau1 in DIV5 (top) and DIV3 (bottom) HNs. Left, Tau1 (axon marker, magenta); middle, AKAP9 (cyan); right, overlay. Scale bar, 50 µm ...View More

Gorshkov, K., Mehta, S., Ramamurthy, S., Ronnett, G. V., Zhou, F. Q., & Zhang, J. (2017). AKAP-mediated feedback control of cAMP gradients in developing hippocampal neurons. Nature chemical biology, 13(4), 425-431.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

V2-12556

Application

WB, ICC, IF, IHC, IHC-P, IP

Immunogen

Synthetic peptide corresponding to residues Q(31) F R Q R K A Q S D G Q S P S(45) of human AKAP450

Host Species

Mouse

Specificity

Human, Mouse

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	2 μg/ml
IF(ICC)	4 μg/ml
IHC-P	1:10-1:100
IP	1:10-1:500
IHC	1:10-1:100

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 1 mg/ml BSA

Preservative

0.05% sodium azide

Concentration

1 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

A kinase (PRKA) anchor protein (yotiao) 9

Introduction

The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This is a scaffolding protein that assembles several protein kinases and phosphatases on the centrosome and Golgi apparatus.

Entrez Gene ID

Human	10142
Mouse	100986

UniProt ID

Human	Q99996
Mouse	Q70FJ1

Alternative Names

LQT11; PRKA9; AKAP-9; CG-NAP; YOTIAO; AKAP350; AKAP450; PPP1R45; HYPERION; MU-RMS-40.16A

Function

Scaffolding protein that assembles several protein kinases and phosphatases on the centrosome and Golgi apparatus. Required to maintain the integrity of the Golgi apparatus (PubMed:10202149, PubMed:15047863). Required for microtubule nucleation at the cis-side of the Golgi apparatus (PubMed:15047863, PubMed:19242490). Required for association of the centrosomes with the poles of the bipolar mitotic spindle during metaphase (PubMed:25657325). In complex with PDE4DIP isoform 13/MMG8/SMYLE, recruits CAMSAP2 to the Golgi apparatus and tethers non-centrosomal minus-end microtubules to the Golgi, an important step for polarized cell movement (PubMed:27666745, PubMed:28814570). In complex with PDE4DIP isoform 13/MMG8/SMYLE, EB1/MAPRE1 and CDK5RAP2, contributes to microtubules nucleation and extension also from the centrosome to the cell periphery (PubMed:29162697).
Isoform 4: Associated with the N-methyl-D-aspartate receptor and is specifically found in the neuromuscular junction (NMJ) as well as in neuronal synapses, suggesting a role in the organization of postsynaptic specializations.

Biological Process

Cardiac conduction Source: Reactome
Cellular response to cAMP Source: BHF-UCL
Chemical synaptic transmission Source: ProtInc
Ciliary basal body-plasma membrane docking Source: Reactome
G2/M transition of mitotic cell cycle Source: Reactome
Maintenance of centrosome location Source: UniProtKB
Microtubule nucleation Source: UniProtKB
Positive regulation of microtubule polymerization Source: UniProtKB
Positive regulation of peptidyl-serine phosphorylation Source: BHF-UCL
Positive regulation of potassium ion transmembrane transporter activity Source: BHF-UCL
Regulation of cardiac muscle cell action potential involved in regulation of contraction Source: BHF-UCL
Regulation of G2/M transition of mitotic cell cycle Source: Reactome
Regulation of Golgi organization Source: UniProtKB
Regulation of heart rate by cardiac conduction Source: BHF-UCL
Regulation of membrane repolarization Source: BHF-UCL
Regulation of postsynaptic neurotransmitter receptor activity Source: SynGO
Regulation of ventricular cardiac muscle cell membrane repolarization Source: BHF-UCL
Signal transduction Source: ProtInc

Cellular Location

Centrosome; Golgi apparatus; Cytoplasm. Cytoplasmic in parietal cells (PubMed:9915845). Recruited to the Golgi apparatus by GM130/GOLGA2 (PubMed:25657325). Localization at the centrosome versus Golgi apparatus may be cell line-dependent. In SKBr3 and HEK293F cells, exclusively located at the centrosome (PubMed:29162697). In HeLa, MDA-MB231 and RPE-1 cells, detected at the Golgi apparatus (PubMed:25217626, PubMed:29162697). In SK-BR-3 cells, recruited to the centrosome in the presence of CDK5RAP2 (PubMed:29162697).

Involvement in disease

Long QT syndrome 11 (LQT11): A heart disorder characterized by a prolonged QT interval on the ECG and polymorphic ventricular arrhythmias. They cause syncope and sudden death in response to exercise or emotional stress, and can present with a sentinel event of sudden cardiac death in infancy.

Bottigliero, D., Monaco, I., Santacroce, R., Casavecchia, G., Correale, M., Guastafierro, F., ... & Brunetti, N. D. (2019). Novel AKAP9 mutation and long QT syndrome in a patient with torsades des pointes. Journal of Interventional Cardiac Electrophysiology, 56(2), 171-172.

Ikezu, T., Chen, C., DeLeo, A. M., Zeldich, E., Fallin, M. D., Kanaan, N. M., ... & Farrer, L. A. (2018). Tau phosphorylation is impacted by rare AKAP9 mutations associated with Alzheimer disease in African Americans. Journal of Neuroimmune Pharmacology, 13(2), 254-264.

Trivedi, A. N. (2017). Association of type 1 inositol 1, 4, 5-trisphosphate receptor (IP₃R1) with protein kinase A and A-kinase Anchoring Protein 9 (AKAP9) in platelets (Doctoral dissertation, University of Hull and University of York).

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Related Products

Mouse Anti-AKAP9 Recombinant Antibody (V2-12558) (CAT#: CBMAB-1048-CN)

Mouse Anti-AKAP9 Recombinant Antibody (V2-12557) (CAT#: CBMAB-1047-CN)

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For research use only. Not intended for any clinical use.

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