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Mouse Anti-Arc Recombinant Antibody (CBYC-A734) (CBMAB-A3405-YC)

Provided herein is a Mouse monoclonal antibody against Mouse Activity Regulated Cytoskeleton Associated Protein. The antibody can be used for immunoassay techniques, such as IP, ELISA.
See all Arc antibodies
Published Data
Mouse Anti-Arc Recombinant Antibody (CBYC-A734) CBMAB-A3405-YC in WB
Total cell lysates were analyzed by western blotting with the antibodies indicated next to the corresponding blot (n = 2). ...View More
Gandin, V., English, B. P., Freeman, M., Leroux, L. P., Preibisch, S., Walpita, D., ... & Singer, R. H. (2022). Cap-dependent translation initiation monitored in living cells. Nature communications, 13(1), 6558.
Mouse Anti-Arc Recombinant Antibody (CBYC-A734) CBMAB-A3405-YC in IF
Representative immunofluorescence (4× magnification) staining for Arc in the hippocampus. ...View More
Li, T., Xu, G., Yi, J., & Huang, Y. (2022). Intraoperative Hypothermia Induces Vascular Dysfunction in the CA1 Region of Rat Hippocampus. Brain Sciences, 12(6), 692.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBYC-A734
Antibody Isotype
IgG2a, κ
Application
ELISA, WB, IF, IP

Basic Information

Immunogen
Amino acids 1-300 of Arc of human origin.
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Activity Regulated Cytoskeleton Associated Protein
Introduction
Arc is required for consolidation of synaptic plasticity as well as formation of long-term memory. Arc regulates endocytosis of AMPA receptors in response to synaptic activity and is required for homeostatic synaptic scaling of AMPA receptors. Arc plays a
Entrez Gene ID
Mouse11838
Rat54323
UniProt ID
MouseQ9WV31
RatQ63053
Alternative Names
Activity Regulated Cytoskeleton Associated Protein; Activity-Regulated Gene 3.1 Protein Homolog; ARC/ARG3.1; Arg3.1; Activity-Regulated Cytoskeleton-Associated Protein; KIAA0278;
Function
Master regulator of synaptic plasticity that self-assembles into virion-like capsids that encapsulate RNAs and mediate intercellular RNA transfer in the nervous system. ARC protein is released from neurons in extracellular vesicles that mediate the transfer of ARC mRNA into new target cells, where ARC mRNA can undergo activity-dependent translation. ARC capsids are endocytosed and are able to transfer ARC mRNA into the cytoplasm of neurons. Acts as a key regulator of synaptic plasticity: required for protein synthesis-dependent forms of long-term potentiation (LTP) and depression (LTD) and for the formation of long-term memory. Regulates synaptic plasticity by promoting endocytosis of AMPA receptors (AMPARs) in response to synaptic activity: this endocytic pathway maintains levels of surface AMPARs in response to chronic changes in neuronal activity through synaptic scaling, thereby contributing to neuronal homeostasis. Acts as a postsynaptic mediator of activity-dependent synapse elimination in the developing cerebellum by mediating elimination of surplus climbing fiber synapses. Accumulates at weaker synapses, probably to prevent their undesired enhancement. This suggests that ARC-containing virion-like capsids may be required to eliminate synaptic material. Required to transduce experience into long-lasting changes in visual cortex plasticity and for long-term memory (By similarity).
Involved in postsynaptic trafficking and processing of amyloid-beta A4 (APP) via interaction with PSEN1 (By similarity).
In addition to its role in synapses, also involved in the regulation of the immune system: specifically expressed in skin-migratory dendritic cells and regulates fast dendritic cell migration, thereby regulating T-cell activation (By similarity).
Biological Process
Anterior/posterior pattern specification Source: Ensembl
Cell migration Source: UniProtKB
Cytoskeleton organization Source: UniProtKB
Dendritic spine morphogenesis Source: ARUK-UCL
Endocytosis Source: UniProtKB-KW
Endoderm development Source: Ensembl
Learning Source: Ensembl
Long-term memory Source: UniProtKB
Long-term synaptic potentiation Source: ARUK-UCL
Modulation of chemical synaptic transmission Source: UniProtKB
mRNA transport Source: UniProtKB
Positive regulation of AMPA receptor activity Source: GO_Central
Protein homooligomerization Source: UniProtKB
Regulation of cell morphogenesis Source: UniProtKB
Regulation of dendritic spine morphogenesis Source: ARUK-UCL
Regulation of long-term synaptic depression Source: UniProtKB
Regulation of long-term synaptic potentiation Source: UniProtKB
Regulation of neuronal synaptic plasticity Source: GO_Central
Regulation of postsynaptic neurotransmitter receptor internalization Source: Ensembl
Vesicle-mediated intercellular transport Source: UniProtKB
Cellular Location
Postsynaptic cell membrane; Early endosome membrane; Cytoskeleton; Extracellular vesicle membrane; Synapse; Postsynaptic density; Dendrite; Cell cortex; Dendritic spine; Acrosome. Forms virion-like extracellular vesicles that are released from neurons. Enriched in postsynaptic density of dendritic spines. Targeted to inactive synapses following interaction with CAMK2B in the kinase inactive state. Accumulation at weaker synapses may be required to prevent their undesired enhancement. Associated with the cell cortex of neuronal soma and dendrites (By similarity). Associated with the sperm tail (By similarity).
PTM
Palmitoylation anchors the protein into the membrane by allowing direct insertion into the hydrophobic core of the lipid bilayer.
Ubiquitinated by UBE3A, leading to its degradation by the proteasome, thereby promoting AMPA receptors (AMPARs) expression at synapses.
Phosphorylation at Ser-260 by CaMK2 prevents homooligomerization into virion-like capsids by disrupting an interaction surface essential for high-order oligomerization. Phosphorylation by CaMK2 inhibits synaptic activity.

Nielsen, L. D., Pedersen, C. P., Erlendsson, S., & Teilum, K. (2019). The capsid domain of Arc changes its oligomerization propensity through direct interaction with the NMDA receptor. Structure, 27(7), 1071-1081.

Park, A. Y., Park, Y. S., So, D., Song, I. K., Choi, J. E., Kim, H. J., & Lee, K. J. (2019). Activity-regulated cytoskeleton-associated protein (Arc/Arg3. 1) is transiently expressed after heat shock stress and suppresses heat shock factor 1. Scientific reports, 9(1), 1-13.

Calabrò, M., Fabbri, C., Crisafulli, C., Albani, D., Forloni, G., Kasper, S., ... & Serretti, A. (2018). The serotonin transporter and the activity regulated cytoskeleton‐associated protein genes in antidepressant response and resistance: 5‐HTTLPR and other variants. Human Psychopharmacology: Clinical and Experimental, 33(6), e2682.

Mabb, A. M., & Ehlers, M. D. (2018, May). Arc ubiquitination in synaptic plasticity. In Seminars in cell & developmental biology (Vol. 77, pp. 10-16). Academic Press.

Newpher, T. M., Harris, S., Pringle, J., Hamilton, C., & Soderling, S. (2018, May). Regulation of spine structural plasticity by Arc/Arg3. 1. In Seminars in cell & developmental biology (Vol. 77, pp. 25-32). Academic Press.

Wall, M. J., & Corrêa, S. A. (2018, May). The mechanistic link between Arc/Arg3. 1 expression and AMPA receptor endocytosis. In Seminars in cell & developmental biology (Vol. 77, pp. 17-24). Academic Press.

Hallin, E. I., Eriksen, M. S., Baryshnikov, S., Nikolaienko, O., Grødem, S., Hosokawa, T., ... & Kursula, P. (2018). Structure of monomeric full‐length ARC sheds light on molecular flexibility, protein interactions, and functional modalities. Journal of neurochemistry, 147(3), 323-343.

Salery, M., Dos Santos, M., Saint-Jour, E., Moumné, L., Pagès, C., Kappès, V., ... & Vanhoutte, P. (2017). Activity-regulated cytoskeleton-associated protein accumulates in the nucleus in response to cocaine and acts as a brake on chromatin remodeling and long-term behavioral alterations. Biological Psychiatry, 81(7), 573-584.

Nikolaienko, O., Eriksen, M. S., Patil, S., Bito, H., & Bramham, C. R. (2017). Stimulus-evoked ERK-dependent phosphorylation of activity-regulated cytoskeleton-associated protein (Arc) regulates its neuronal subcellular localization. Neuroscience, 360, 68-80.

Gozdz, A., Nikolaienko, O., Urbanska, M., Cymerman, I. A., Sitkiewicz, E., Blazejczyk, M., ... & Jaworski, J. (2017). GSK3α and GSK3β phosphorylate Arc and regulate its degradation. Frontiers in molecular neuroscience, 10, 192.

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For research use only. Not intended for any clinical use.

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