Mouse Anti-ARPC3 Recombinant Antibody (26/p21-Arc) (CBMAB-P0074-YC)

Name: Mouse Anti-ARPC3 Recombinant Antibody (26/p21-Arc)
SKU: CBMAB-P0074-YC
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-ARPC3 Recombinant Antibody (26/p21-Arc) CBMAB-P0074-YC in WB

Western blot analysis of indicated proteins in cells with or without genipin (30 or 60 µM). Bar graphs demonstrate densitometric analysis of western blots from three independent experiments. *P<0.05 vs. vehicle-treated cells. ...View More

Li, Z., Zhang, T. B., Jia, D. H., Sun, W. Q., Wang, C. L., Gu, A. Z., & Yang, X. M. (2018). Genipin inhibits the growth of human bladder cancer cells via inactivation of PI3K/Akt signaling. Oncology letters, 15(2), 2619-2624.

Mouse Anti-ARPC3 Recombinant Antibody (26/p21-Arc) CBMAB-P0074-YC in WB

Western blot analysis of p21, p-Rb, CDK4, CDK6, cyclin D1 and cyclin A2 expression in DU145 cells indicated that overexpression of miR-1180 regulated the levels of these proteins. *P<0.05 and **P<0.01, vs. corresponding control group. ...View More

Zhu, D., Gao, W., & Zhang, Z. (2018). MicroRNA-1180 is associated with growth and apoptosis in prostate cancer via TNF receptor associated factor 1 expression regulation and nuclear factor-κB signaling pathway activation. Oncology letters, 15(4), 4775-4780.

Mouse Anti-ARPC3 Recombinant Antibody (26/p21-Arc) CBMAB-P0074-YC in WB

Expression of PRMT6, p21, CDK2, FAK and SRC protein was assessed by western blot analysis. ...View More

Zhao, X., Zhou, D., Liu, Y., Li, C., Zhao, X., Li, Y., & Li, W. (2018). Ganoderma lucidum polysaccharide inhibits prostate cancer cell migration via the protein arginine methyltransferase 6 signaling pathway. Molecular medicine reports, 17(1), 147-157.

Datasheet

SDS

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Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

26/p21-Arc

Application

WB, IP, IF

Immunogen

Amino acids 10-118 of p21-ARC of human origin.

Specificity

Human, Dog, Mouse, Rat

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.1 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

actin related protein 2/3 complex, subunit 3, 21kDa

Introduction

ARPC3 is one of seven subunits of the human Arp2/3 protein complex. The Arp2/3 protein complex has been conserved through evolution and is implicated in the control of actin polymerization in cells.

Entrez Gene ID

Human	10094
Mouse	56378
Rat	288669
Chicken	416837
Dog	100856747

UniProt ID

Human	O15145
Mouse	Q9JM76
Rat	B2GV73
Chicken	E1C8Y3
Dog	E2R985

Alternative Names

Actin Related Protein 2/3 Complex Subunit 3; Arp2/3 Complex 21 KDa Subunit; P21-Arc; ARC21; Actin Related Protein 2/3 Complex, Subunit 3 (21 KD); Actin Related Protein 2/3 Complex, Subunit 3, 21kDa; Actin Related Protein 2/3 Complex Subunit 3, 21kDa; Actin-Related Protein 2/3 Complex Subunit 3; ARP2/3 Protein Complex Subunit P21;

Function

Component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF) (PubMed:9230079).
The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility (PubMed:9230079).
In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA (PubMed:29925947).
The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs) (PubMed:29925947).

Biological Process

Actin polymerization-dependent cell motility Source: UniProtKB
Arp2/3 complex-mediated actin nucleation Source: FlyBase
Cellular response to nerve growth factor stimulus Source: Ensembl
Ephrin receptor signaling pathway Source: Reactome
Fc-gamma receptor signaling pathway involved in phagocytosis Source: Reactome
Membrane organization Source: Reactome

Cellular Location

Cytoskeleton; Nucleus; Cell projection

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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