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Rabbit Anti-Asymmetric-Methyl-PABPC1 (Arg455/Arg460) Recombinant Antibody (C60A10) (CBMAB-CP1851-LY)

The product is antibody recognizes Asymmetric-Methyl-PABPC1 (Arg455/Arg460). The antibody C60A10 immunoassay techniques such as: WB,IP,IHC-P,IF (ICC).
See all Asymmetric-Methyl-PABPC1 (Arg455/Arg460) antibodies
Published Data
Rabbit Anti-Asymmetric-Methyl-PABPC1 (Arg455/Arg460) Recombinant Antibody (C60A10) (CBMAB-CP1851-LY) in IHC
Immunohistochemistry of cerebellar Purkinje cells. At 7, 14 and 24 months age, the Purkinje cells showed a purely nuclear distribution of large polyQ-domains as revealed by 1C2-staining in WT tissue, while an additional granular cytoplasmic staining pattern became apparent at 14 and 24 months in knock-in tissue (A-F). ATXN2 immunoreactivity was diffusely distributed throughout the cytoplasm and concentrated in the perinuclear region in WT tissue, while a more granular appearance was detected discretely at 14 and markedly at 24 months in CAG42 mice (G-L). The expected diffuse cytoplasmic distribution of PABPC1 was documented in WT mice; whereas again a more granular staining pattern in CAG42 mice appeared by 14 months (M-R). ...View More
Damrath, E., Heck, M. V., Gispert, S., Azizov, M., Nowock, J., Seifried, C., ... & Auburger, G. (2012). ATXN2-CAG42 sequesters PABPC1 into insolubility and induces FBXW8 in cerebellum of old ataxic knock-in mice.
Rabbit Anti-Asymmetric-Methyl-PABPC1 (Arg455/Arg460) Recombinant Antibody (C60A10) (CBMAB-CP1851-LY) in WB
Representative Western blots of BAF155 arginine asymmetric dimethylation (BAF155me2a), total BAF155, arginine asymmetric dimethylation of PABP1 (PABP1me2a), total PABP1, arginine methylated CARM1 substrates, ADMA at glycine and arginine-rich motifs (ADMA), and SDMA in TA muscles from WT and mKO mice. The ponceau stain demonstrates equal loading while approximate MWs (kda) are to the right of each image. ...View More
Stouth, D. W., Hartel, N. G., Vasam, G., Ng, S. Y., Webb, E. K., Rebalka, I. A., ... & Ljubicic, V. (2022). The CARM1 transcriptome and arginine methylproteome mediate skeletal muscle integrative biology. Molecular Metabolism, 64, 101555.

Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat, Monkey
Clone
C60A10
Antibody Isotype
IgG
Application
WB, IP, IHC-P, IF

Basic Information

Immunogen
Synthetic peptide corresponding to the sequence of human PABP1 in which Arg455 and Arg460 are methylated.
Host Species
Mouse
Specificity
Human, Mouse, Rat, Monkey
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:1,000
IHC-P1:1,000
IP1:25
IF(ICC)1:400

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
HEPES, pH 7.5, 100 µg/ml BSA, 50% glycerol
Preservative
0.02% sodium azide
Concentration
Batch dependent
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
poly(A) binding protein cytoplasmic 1
Introduction
This gene encodes a poly(A) binding protein. The protein shuttles between the nucleus and cytoplasm and binds to the 3' poly(A) tail of eukaryotic messenger RNAs via RNA-recognition motifs. The binding of this protein to poly(A) promotes ribosome recruitment and translation initiation; it is also required for poly(A) shortening which is the first step in mRNA decay. The gene is part of a small gene family including three protein-coding genes and several pseudogenes.
Entrez Gene ID
Human6599
Mouse20588
Rat301020
Monkey711134
Chicken430997
Horse100056021
UniProt ID
HumanQ92922
MouseP97496
RatD3ZJU5
MonkeyF7HJG9
ChickenA0A1D5NYB2
HorseA0A3Q2HKM4
Alternative Names
PAB1; PABP; PABP1; PABPC2; PABPL1
Function
Binds the poly(A) tail of mRNA, including that of its own transcript, and regulates processes of mRNA metabolism such as pre-mRNA splicing and mRNA stability (PubMed:11051545, PubMed:17212783, PubMed:25480299).
Its function in translational initiation regulation can either be enhanced by PAIP1 or repressed by PAIP2 (PubMed:11051545, PubMed:20573744).
Can probably bind to cytoplasmic RNA sequences other than poly(A) in vivo. Involved in translationally coupled mRNA turnover (PubMed:11051545).
Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain (PubMed:11051545).
Involved in regulation of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons; for the recognition of premature termination codons (PTC) and initiation of NMD a competitive interaction between UPF1 and PABPC1 with the ribosome-bound release factors is proposed (PubMed:18447585).
By binding to long poly(A) tails, may protect them from uridylation by ZCCHC6/ZCCHC11 and hence contribute to mRNA stability (PubMed:25480299).
(Microbial infection) Positively regulates the replication of dengue virus (DENV).
Biological Process
Gene silencing by RNA Source: UniProtKB
mRNA polyadenylation Source: UniProtKB
mRNA splicing, via spliceosome Source: UniProtKB
mRNA stabilization Source: UniProtKB
Negative regulation of nuclear-transcribed mRNA catabolic process, nonsense-mediated decay Source: UniProtKB
Nuclear-transcribed mRNA catabolic process, nonsense-mediated decay Source: Reactome
Positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay Source: UniProtKB
Positive regulation of nuclear-transcribed mRNA poly(A) tail shortening Source: UniProtKB
Positive regulation of viral genome replication Source: UniProtKB
Regulation of mRNA stability Source: Reactome
Translational initiation Source: Reactome
Cellular Location
Cytoplasm; Nucleus; Stress granule; Lamellipodium. Localized in cytoplasmic mRNP granules containing untranslated mRNAs (PubMed:17289661). Shuttles between the cytoplasm and the nucleus (PubMed:9582337). During stress and in the absence of DDX3X, localizes to the nucleus (PubMed:21883093). At the leading edge of migrating fibroblasts, colocalizes with DDX3X (PubMed:28733330). Relocalizes to cytoplasmic stress granules upon cellular stress where it colocalizes with ENDOV (PubMed:27573237). In case of HRSV infection, localizes in cytoplasmic inclusion bodies substructures called inclusion bodies associated granules (IBAGs) (PubMed:31649314).
PTM
Phosphorylated by MAPKAPK2.
Methylated by CARM1. Arg-493 is dimethylated, probably to asymmetric dimethylarginine.
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For research use only. Not intended for any clinical use.

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