Rabbit Anti-Asymmetric-Methyl-PABPC1 (Arg455/Arg460) Recombinant Antibody (C60A10) (CBMAB-CP1851-LY)

Name: Rabbit Anti-Asymmetric-Methyl-PABPC1 (Arg455/Arg460) Recombinant Antibody (C60A10)
SKU: CBMAB-CP1851-LY
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Published Data

Rabbit Anti-Asymmetric-Methyl-PABPC1 (Arg455/Arg460) Recombinant Antibody (C60A10) (CBMAB-CP1851-LY) in IHC

Immunohistochemistry of cerebellar Purkinje cells. At 7, 14 and 24 months age, the Purkinje cells showed a purely nuclear distribution of large polyQ-domains as revealed by 1C2-staining in WT tissue, while an additional granular cytoplasmic staining pattern became apparent at 14 and 24 months in knock-in tissue (A-F). ATXN2 immunoreactivity was diffusely distributed throughout the cytoplasm and concentrated in the perinuclear region in WT tissue, while a more granular appearance was detected discretely at 14 and markedly at 24 months in CAG42 mice (G-L). The expected diffuse cytoplasmic distribution of PABPC1 was documented in WT mice; whereas again a more granular staining pattern in CAG42 mice appeared by 14 months (M-R). ...View More

Damrath, E., Heck, M. V., Gispert, S., Azizov, M., Nowock, J., Seifried, C., ... & Auburger, G. (2012). ATXN2-CAG42 sequesters PABPC1 into insolubility and induces FBXW8 in cerebellum of old ataxic knock-in mice.

Rabbit Anti-Asymmetric-Methyl-PABPC1 (Arg455/Arg460) Recombinant Antibody (C60A10) (CBMAB-CP1851-LY) in WB

Representative Western blots of BAF155 arginine asymmetric dimethylation (BAF155me2a), total BAF155, arginine asymmetric dimethylation of PABP1 (PABP1me2a), total PABP1, arginine methylated CARM1 substrates, ADMA at glycine and arginine-rich motifs (ADMA), and SDMA in TA muscles from WT and mKO mice. The ponceau stain demonstrates equal loading while approximate MWs (kda) are to the right of each image. ...View More

Stouth, D. W., Hartel, N. G., Vasam, G., Ng, S. Y., Webb, E. K., Rebalka, I. A., ... & Ljubicic, V. (2022). The CARM1 transcriptome and arginine methylproteome mediate skeletal muscle integrative biology. Molecular Metabolism, 64, 101555.

Datasheet

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Basic Information

Host Animal

Rabbit

Clone

C60A10

Application

WB, IP, IHC-P, IF

Immunogen

Synthetic peptide corresponding to the sequence of human PABP1 in which Arg455 and Arg460 are methylated.

Host Species

Mouse

Specificity

Human, Mouse, Rat, Monkey

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:1,000
IHC-P	1:1,000
IP	1:25
IF(ICC)	1:400

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

HEPES, pH 7.5, 100 µg/ml BSA, 50% glycerol

Preservative

0.02% sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

poly(A) binding protein cytoplasmic 1

Introduction

This gene encodes a poly(A) binding protein. The protein shuttles between the nucleus and cytoplasm and binds to the 3' poly(A) tail of eukaryotic messenger RNAs via RNA-recognition motifs. The binding of this protein to poly(A) promotes ribosome recruitment and translation initiation; it is also required for poly(A) shortening which is the first step in mRNA decay. The gene is part of a small gene family including three protein-coding genes and several pseudogenes.

Entrez Gene ID

Human	6599
Mouse	20588
Rat	301020
Monkey	711134
Chicken	430997
Horse	100056021

UniProt ID

Human	Q92922
Mouse	P97496
Rat	D3ZJU5
Monkey	F7HJG9
Chicken	A0A1D5NYB2
Horse	A0A3Q2HKM4

Alternative Names

PAB1; PABP; PABP1; PABPC2; PABPL1

Function

Binds the poly(A) tail of mRNA, including that of its own transcript, and regulates processes of mRNA metabolism such as pre-mRNA splicing and mRNA stability (PubMed:11051545, PubMed:17212783, PubMed:25480299).
Its function in translational initiation regulation can either be enhanced by PAIP1 or repressed by PAIP2 (PubMed:11051545, PubMed:20573744).
Can probably bind to cytoplasmic RNA sequences other than poly(A) in vivo. Involved in translationally coupled mRNA turnover (PubMed:11051545).
Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain (PubMed:11051545).
Involved in regulation of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons; for the recognition of premature termination codons (PTC) and initiation of NMD a competitive interaction between UPF1 and PABPC1 with the ribosome-bound release factors is proposed (PubMed:18447585).
By binding to long poly(A) tails, may protect them from uridylation by ZCCHC6/ZCCHC11 and hence contribute to mRNA stability (PubMed:25480299).
(Microbial infection) Positively regulates the replication of dengue virus (DENV).

Biological Process

Gene silencing by RNA Source: UniProtKB
mRNA polyadenylation Source: UniProtKB
mRNA splicing, via spliceosome Source: UniProtKB
mRNA stabilization Source: UniProtKB
Negative regulation of nuclear-transcribed mRNA catabolic process, nonsense-mediated decay Source: UniProtKB
Nuclear-transcribed mRNA catabolic process, nonsense-mediated decay Source: Reactome
Positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay Source: UniProtKB
Positive regulation of nuclear-transcribed mRNA poly(A) tail shortening Source: UniProtKB
Positive regulation of viral genome replication Source: UniProtKB
Regulation of mRNA stability Source: Reactome
Translational initiation Source: Reactome

Cellular Location

Cytoplasm; Nucleus; Stress granule; Lamellipodium. Localized in cytoplasmic mRNP granules containing untranslated mRNAs (PubMed:17289661). Shuttles between the cytoplasm and the nucleus (PubMed:9582337). During stress and in the absence of DDX3X, localizes to the nucleus (PubMed:21883093). At the leading edge of migrating fibroblasts, colocalizes with DDX3X (PubMed:28733330). Relocalizes to cytoplasmic stress granules upon cellular stress where it colocalizes with ENDOV (PubMed:27573237). In case of HRSV infection, localizes in cytoplasmic inclusion bodies substructures called inclusion bodies associated granules (IBAGs) (PubMed:31649314).

PTM

Phosphorylated by MAPKAPK2.
Methylated by CARM1. Arg-493 is dimethylated, probably to asymmetric dimethylarginine.

Pu, J., Zhang, T., Zhang, D., He, K., Chen, Y., Sun, X., & Long, W. (2021). High-Expression of Cytoplasmic Poly (A) Binding Protein 1 (PABPC1) as a Prognostic Biomarker for Early-Stage Esophageal Squamous Cell Carcinoma. Cancer Management and Research, 13, 5361.

Amine, H., Ripin, N., Sharma, S., Stoecklin, G., Allain, F. H., Séraphin, B., & Mauxion, F. (2021). A conserved motif in human BTG1 and BTG2 proteins mediates interaction with the poly (A) binding protein PABPC1 to stimulate mRNA deadenylation. RNA biology, 1-16.

Graetz, D., Crews, K. R., Azzato, E. M., Singh, R. K., Raimondi, S., Mason, J., ... & Leventaki, V. (2019). Leukemic presentation of ALK-positive anaplastic large cell lymphoma with a novel partner, poly (A) binding protein cytoplasmic 1 (PABPC1), responding to single-agent crizotinib. haematologica, 104(5), e218.

Peng, Y., Yuan, J., Zhang, Z., & Chang, X. (2017). Cytoplasmic poly (A)-binding protein 1 (PABPC1) interacts with the RNA-binding protein hnRNPLL and thereby regulates immunoglobulin secretion in plasma cells. Journal of Biological Chemistry, 292(29), 12285-12295.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
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For research use only. Not intended for any clinical use.

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