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Rabbit Anti-ATF1 (Phosphorylated Ser133, Ser63) Recombinant Antibody (PTM016YC) (PTM-CBMAB-0046YC)

Provided herein is a Rabbit monoclonal antibody against Activating Transcription Factor 1. The antibody can be used for immunoassay techniques, such as ELISA, ICC, IF, IHC, IHC-P, WB.
See all ATF1 antibodies

Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Dog
Clone
PTM016YC
Antibody Isotype
IgG
Application
ELISA, ICC, IF, IHC, IHC-P, WB

Basic Information

Immunogen
A synthetic phosphopeptide corresponding to amino acids 125-135 surrounding the Ser133 phosphorylation site of human CREB.
Specificity
Human, Mouse, Dog
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
IF(ICC)1:20-1:200
WB1:500
ELISA0.05 µg/ml
IHC-P1:50-1:500
FC1 µg/test

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.2, 50% glycerol
Preservative
0.09% sodium azide
Concentration
0.1 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Activating Transcription Factor 1
Introduction
ATF1 is an activating transcription factor, which belongs to the ATF subfamily and bZIP (basic-region leucine zipper) family. It influences cellular physiologic processes by regulating the expression of downstream target genes, which are related to growth, survival, and other cellular activities. This protein is phosphorylated at serine 63 in its kinase-inducible domain by serine/threonine kinases, cAMP-dependent protein kinase A, calmodulin-dependent protein kinase I/II, mitogen- and stress-activated protein kinase and cyclin-dependent kinase 3 (cdk-3). Its phosphorylation enhances its transactivation and transcriptional activities, and enhances cell transformation. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in angiomatoid fibrous histiocytoma and clear cell sarcoma. This gene has a pseudogene on chromosome 6.
Entrez Gene ID
Human466
Mouse11908
Rat315305
UniProt ID
HumanP18846
MouseP81269
RatQ5BK34
Alternative Names
Activating Transcription Factor 1; CAMP-Dependent Transcription Factor ATF-1; Cyclic AMP-Dependent Transcription Factor ATF-1; Protein TREB36; FUS/ATF-1; EWS-ATF1; TREB36;
Function
This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Binds to the Tax-responsive element (TRE) of HTLV-I. Mediates PKA-induced stimulation of CRE-reporter genes. Represses the expression of FTH1 and other antioxidant detoxification genes. Triggers cell proliferation and transformation.
Biological Process
Cellular protein-containing complex assembly Source: Ensembl
Positive regulation of DNA replication Source: Ensembl
Positive regulation of neuron projection development Source: Ensembl
Positive regulation of transcription by RNA polymerase II Source: NTNU_SB
Regulation of transcription by RNA polymerase II Source: GO_Central
Response to cobalt ion Source: Ensembl
Response to organic cyclic compound Source: Ensembl
Cellular Location
Nucleus
Involvement in disease
Angiomatoid fibrous histiocytoma (AFH): The gene represented in this entry may be involved in disease pathogenesis. Chromosomal aberrations involving ATF1 are found in patients with angiomatoid fibrous histiocytoma. Translocation t(12;16)(q13;p11.2) with FUS generates a chimeric ATF1/FUS protein. Translocation t(12;22)(q13;q12) with EWSR1 generates a chimeric ATF1/EWSR1 protein. A distinct variant of malignant fibrous histiocytoma that typically occurs in children and adolescents and is manifest by nodular subcutaneous growth. Characteristic microscopic features include lobulated sheets of histiocyte-like cells intimately associated with areas of hemorrhage and cystic pseudovascular spaces, as well as a striking cuffing of inflammatory cells, mimicking a lymph node metastasis.
PTM
Phosphorylated at Ser-198 by HIPK2 in response to genotoxic stress. This phosphorylation promotes transcription repression of FTH1 and other antioxidant detoxification genes. The CDK3-mediated phosphorylation at Ser-63 promotes its transactivation and transcriptional activities. Phosphorylated at Ser-63 by RPS6KA4 and RPS6KA5 in response to mitogenic or stress stimuli.

Tabara, M., Shiraishi, K., Takii, R., Fujimoto, M., Nakai, A., & Matsuyama, H. (2021). Testicular localization of activating transcription factor 1 and its potential function during spermatogenesis. Biology of Reproduction.

Seneviratne, A., Han, Y., Wong, E., Walter, E. R., Jiang, L., Cave, L., ... & Boyle, J. J. (2020). Hematoma resolution in vivo is directed by activating transcription factor 1. Circulation research, 127(7), 928-944.

Ge, Y., Cave, L., Seneviratne, A., Carling, D., Mason, J. C., Haskard, D., & Boyle, J. J. (2020). Switching macrophage gene expression from inflammation-resolution to hemorrhage-resolution by redirection of activating transcription factor 1 (ATF1) binding by SMARCA4, BACH1 and histone H3K9 acetylation. Atherosclerosis, 315, e2.

Wu, L., Guo, Y., Cai, S., Kuang, L., Shen, Q., Wu, D., & Zhang, G. (2020). The zinc finger transcription factor ATF1 regulates aluminum tolerance in barley. Journal of Experimental Botany, 71(20), 6512-6523.

Cui, J., Yin, Z., Liu, G., Chen, X., Gao, X., Lu, H., ... & Lin, D. (2019). Activating transcription factor 1 promoted migration and invasion in lung cancer cells through regulating EGFR and MMP‐2. Molecular carcinogenesis, 58(10), 1919-1924.

Boyle, J., Seneviratne, A., Han, Y., Jiang, L., Walter, E., Cave, L., ... & Haskard, D. O. (2019). Vertebrate Hematoma Resolution Is Directed By Activating Transcription Factor 1 (Atf1) And Adenosine-Monophosphate-Activated-Protein-Kinase (Ampk). Atherosclerosis, 287, e246.

Boyle, J., Seneviratne, A., Tsao, A., Shaikh, A., Carling, D., Haskard, D. O., & Cave, L. (2019). Smarca4 Redirects Binding Of Macrophage Activating Transcription Factor 1 (Atf1) From Genes For Inflammation Resolution To Genes For Erythrocyte Resolution. Atherosclerosis, 287, e78.

Hao, Q., Zhao, X., Zhang, Y., Dong, Z., Hu, T., & Chen, P. (2017). Targeting overexpressed activating transcription factor 1 (ATF1) inhibits proliferation and migration and enhances sensitivity to paclitaxel in esophageal cancer cells. Medical science monitor basic research, 23, 304.

Shi, Y., Wang, W., Yang, B., & Tian, H. (2017). ATF1 and RAS in exosomes are potential clinical diagnostic markers for cervical cancer. Cell biochemistry and function, 35(7), 477-483.

Salat-Canela, C., Paulo, E., Sánchez-Mir, L., Carmona, M., Ayté, J., Oliva, B., & Hidalgo, E. (2017). Deciphering the role of the signal-and Sty1 kinase-dependent phosphorylation of the stress-responsive transcription factor Atf1 on gene activation. Journal of Biological Chemistry, 292(33), 13635-13644.

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For research use only. Not intended for any clinical use.

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