Sign in or Register   Sign in or Register
  |  

Mouse Anti-ATP5PD Recombinant Antibody (E1) (CBMAB-A4069-YC)

Provided herein is a Mouse monoclonal antibody against Human ATP Synthase Peripheral Stalk Subunit D. The antibody can be used for immunoassay techniques, such as ICC, IF.
See all ATP5PD antibodies
Published Data
Mouse Anti-ATP5PD Recombinant Antibody (E1) in Immunoblot
T98G and U343MG cells were subjected to NPe6-PDT (NPe6, 15 μg·mL−1) in the presence or absence of pretreatment with vehicle, 3-ABA (3 mM), or PJ34 (15 μM), as shown in the figure. After 24 h, the lysates of all these treated cells were separated into the mitochondria-enriched membrane fraction, cytosolic fraction, and nuclear fraction, as described in the Materials and Methods. The fractionated cell lysates as well as whole-cell lysates were analyzed by immunoblotting using the indicated primary antibodies. ...View More
Kobayashi, T., Miyazaki, M., Sasaki, N., Yamamuro, S., Uchida, E., Kawauchi, D., ... & Tomiyama, A. (2020). Enhanced malignant phenotypes of glioblastoma cells surviving NPe6-mediated photodynamic therapy are regulated via ERK1/2 activation. Cancers, 12(12), 3641.

Summary

Host Animal
Mouse
Specificity
Human
Clone
E1
Antibody Isotype
IgG1, κ
Application
WB, IP, IHC, IF, ELISA

Basic Information

Immunogen
Amino acids 125-146 near the C-terminus of ATP5H of human origin.
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
ATP Synthase Peripheral Stalk Subunit D
Introduction
Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the
Entrez Gene ID
Human10476
Mouse71679
Rat641434
Cattle282710
Monkey700338
UniProt ID
HumanO75947
MouseQ9DCX2
RatP31399
CattleP13620
MonkeyA0A2K6K943
Alternative Names
ATP Synthase Peripheral Stalk Subunit D; ATP Synthase, H+ Transporting, Mitochondrial Fo Complex Subunit D; ATP5H; ATP Synthase, H+ Transporting, Mitochondrial F0 Complex, Subunit D; ATP Synthase, H+ Transporting, Mitochondrial Fo Complex, Subunit D; ATPa
Function
Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core, and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F0 domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha3beta3 subcomplex and subunit a/ATP6 static relative to the rotary elements.
Biological Process
ATP biosynthetic process Source: Reactome
ATP synthesis coupled proton transport Source: GO_Central
Cellular response to peptide Source: Ensembl
Cristae formation Source: Reactome
Mitochondrial ATP synthesis coupled proton transport Source: UniProtKB
Cellular Location
Mitochondrion; Mitochondrion inner membrane
Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Mouse Anti-ATP5PD Recombinant Antibody (E1)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Go to
Compare