Mouse Anti-BARD1 Recombinant Antibody (CBYY-0196) (CBMAB-0197-YY)

Name: Mouse Anti-BARD1 Recombinant Antibody (CBYY-0196)
SKU: CBMAB-0197-YY
Rating: 5 (1 reviews)

See all BARD1 antibodies

Compare Online Inquiry

Published Data

Mouse Anti-BARD1 Recombinant Antibody (CBYY-0196) in IP

PD-L1 IP from human MBA-MB-231 cells and co-IP lysates blotted for BRCA1 or BARD1. ...View More

Kornepati, A. V., Boyd, J. T., Murray, C. E., Saifetiarova, J., de la Peña Avalos, B., Rogers, C. M., ... & Curiel, T. J. (2022). Tumor intrinsic PD-L1 promotes DNA Repair in distinct cancers and suppresses PARP inhibitor-induced synthetic lethality. Cancer research, 82(11), 2156-2170.

Mouse Anti-BARD1 Recombinant Antibody (CBYY-0196) in IP

HEK293T cells transfected with the indicated plasmids were lysed and the immunoprecipitates were analyzed using western blotting (WB). ...View More

Deng, J., Zhang, T., Liu, F., Han, Q., Li, Q., Guo, X., ... & Shao, G. (2022). CRL4-DCAF8L2 E3 ligase promotes ubiquitination and degradation of BARD1. Biochemical and Biophysical Research Communications, 611, 107-113.

Mouse Anti-BARD1 Recombinant Antibody (CBYY-0196) in IF

Control and DCAF8L2-overexpressing U2OS cells were exposed to X-rays (8 Gy), and fixed 4 h after irradiation. BARD1 foci was examined. Quantifications are averages of three independent experiments (100 cells each), presented as mean ± SD, two-tailed Student's t-test, ∗P < 0.05. Scale bar: 20 μm. ...View More

Mouse Anti-BARD1 Recombinant Antibody (CBYY-0196) in IP and Immunoblot

Cell lysates were immunoprecipitated with anti-BARD1 antibody followed by immunoblotting with anti-BRCA1 and anti-RAD51 antibodies. ...View More

Park, J. W., Park, J. E., Kim, S. R., Sim, M. K., Kang, C. M., & Kim, K. S. (2022). Metformin alleviates ionizing radiation-induced senescence by restoring BARD1-mediated DNA repair in human aortic endothelial cells. Experimental Gerontology, 160, 111706.

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBYY-0196

Application

ELISA, WB, IF, IP, IHC-P

Immunogen

Amino acids 1-300 mapping at the N-terminus of BARD1 of human origin.

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
IHC-P	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

BRCA1 Associated RING Domain 1

Introduction

This gene encodes a protein which interacts with the N-terminal region of BRCA1. In addition to its ability to bind BRCA1 in vivo and in vitro, it shares homology with the 2 most conserved regions of BRCA1: the N-terminal RING motif and the C-terminal BRCT domain. The RING motif is a cysteine-rich sequence found in a variety of proteins that regulate cell growth, including the products of tumor suppressor genes and dominant protooncogenes. This protein also contains 3 tandem ankyrin repeats. The BARD1/BRCA1 interaction is disrupted by tumorigenic amino acid substitutions in BRCA1, implying that the formation of a stable complex between these proteins may be an essential aspect of BRCA1 tumor suppression. This protein may be the target of oncogenic mutations in breast or ovarian cancer. Multiple alternatively spliced transcript variants encoding different isoforMouse have been found for this gene. [provided by RefSeq, Sep 2013]

Entrez Gene ID

580

UniProt ID

Q99728

Alternative Names

BRCA1 Associated RING Domain 1; RING-Type E3 Ubiquitin Transferase BARD1; EC 2.3.2.27; BARD-1;

Function

E3 ubiquitin-protein ligase. The BRCA1-BARD1 heterodimer specifically mediates the formation of 'Lys-6'-linked polyubiquitin chains and coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Plays a central role in the control of the cell cycle in response to DNA damage. Acts by mediating ubiquitin E3 ligase activity that is required for its tumor suppressor function. Also forms a heterodimer with CSTF1/CSTF-50 to modulate mRNA processing and RNAP II stability by inhibiting pre-mRNA 3' cleavage.

Biological Process

Cell cycle arrest Source: UniProtKB
Cellular response to DNA damage stimulus Source: UniProtKB
DNA double-strand break processing Source: Reactome
DNA replication Source: Reactome
Double-strand break repair Source: Reactome
Double-strand break repair via nonhomologous end joining Source: Reactome
Negative regulation of apoptotic process Source: UniProtKB
Negative regulation of mRNA 3'-end processing Source: UniProtKB
Negative regulation of protein export from nucleus Source: UniProtKB
Positive regulation of apoptotic process Source: UniProtKB
Positive regulation of protein catabolic process Source: UniProtKB
Protein deubiquitination Source: Reactome
Protein K6-linked ubiquitination Source: UniProtKB
Protein ubiquitination Source: UniProtKB
Regulation of phosphorylation Source: UniProtKB
Regulation of signal transduction by p53 class mediator Source: Reactome
Tissue homeostasis Source: UniProtKB

Cellular Location

Nucleus. During S phase of the cell cycle, colocalizes with BRCA1 into discrete subnuclear foci. Can translocate to the cytoplasm. Localizes at sites of DNA damage at double-strand breaks (DSBs); recruitment to DNA damage sites is mediated by the BRCA1-A complex.

PTM

Processed during apoptosis. The homodimer is more susceptible to proteolytic cleavage than the BARD1/BRCA1 heterodimer.

Sakai, Y., Hanafusa, H., Shimizu, T., Pastuhov, S. I., Hisamoto, N., & Matsumoto, K. (2021). BRCA1–BARD1 regulates axon regeneration in concert with the Gqα–DAG signaling network. Journal of Neuroscience, 41(13), 2842-2853.

Becker, J. R., Clifford, G., Bonnet, C., Groth, A., Wilson, M. D., & Chapman, J. R. (2021). BARD1 reads H2A lysine 15 ubiquitination to direct homologous recombination. Nature, 1-5.

Tarsounas, M., & Sung, P. (2020). The antitumorigenic roles of BRCA1–BARD1 in DNA repair and replication. Nature Reviews Molecular Cell Biology, 21(5), 284-299.

Watters, A. K., Seltzer, E. S., MacKenzie, D., Young, M., Muratori, J., Hussein, R., ... & Zhang, D. (2020). The Effects of Genetic and Epigenetic Alterations of BARD1 on the Development of Non-Breast and Non-Gynecological Cancers. Genes, 11(7), 829.

Otsuka, K., Yoshino, Y., Qi, H., & Chiba, N. (2020). The Function of BARD1 in Centrosome Regulation in Cooperation with BRCA1/OLA1/RACK1. Genes, 11(8), 842.

Śniadecki, M., Brzeziński, M., Darecka, K., Klasa-Mazurkiewicz, D., Poniewierza, P., Krzeszowiec, M., ... & Wydra, D. (2020). Bard1 and breast cancer: The possibility of creating screening tests and new preventive and therapeutic pathways for predisposed women. Genes, 11(11), 1251.

Alenezi, W. M., Fierheller, C. T., Recio, N., & Tonin, P. N. (2020). Literature review of BARD1 as a cancer predisposing gene with a focus on breast and ovarian cancers. Genes, 11(8), 856.

Nakamura, K., Saredi, G., Becker, J. R., Foster, B. M., Nguyen, N. V., Beyer, T. E., ... & Groth, A. (2019). H4K20me0 recognition by BRCA1–BARD1 directs homologous recombination to sister chromatids. Nature cell biology, 21(3), 311-318.

Cimmino, F., Formicola, D., & Capasso, M. (2017). Dualistic role of BARD1 in cancer. Genes, 8(12), 375.

Liao, Y., Yuan, S., Chen, X., Zhu, P., Li, J., Qin, L., & Liao, W. (2017). Up-regulation of BRCA1-associated RING domain 1 promotes hepatocellular carcinoma progression by targeting Akt signaling. Scientific reports, 7(1), 1-11.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Anti-BARD1 Recombinant Antibody (CBYY-0196)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Related Products

Mouse Anti-BARD1 Recombinant Antibody (EG351) (CAT#: CBMAB-EN388-LY)

Mouse Anti-BARD1 Recombinant Antibody (2A11) (CAT#: CBMAB-A0709-LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry