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Mouse Anti-BIRC2 Recombinant Antibody (422) (CBMAB-C6463-LY)

This product is antibody recognizes BIRC2. The antibody 422 immunoassay techniques such as: WB.
See all BIRC2 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
422
Antibody Isotype
IgG2a
Application
WB, IF

Basic Information

Immunogen
Recombinant protein encompassing a sequence within the center region of human cIAP1.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:500-1:3,000
IF(ICC)1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS
Preservative
None
Concentration
1 mg/ml
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Baculoviral IAP Repeat Containing 2
Introduction
The protein encoded by this gene is a member of a family of proteins that inhibits apoptosis by binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2, probably by interfering with activation of ICE-like proteases. This encoded protein inhibits apoptosis induced by serum deprivation and menadione, a potent inducer of free radicals. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]
Entrez Gene ID
329
UniProt ID
Q13490
Alternative Names
Baculoviral IAP Repeat Containing 2; RING-Type E3 Ubiquitin Transferase BIRC2; TNFR2-TRAF-Signaling Complex Protein 2; NFR2-TRAF Signalling Complex Protein; Cellular Inhibitor Of Apoptosis 1; Inhibitor Of Apoptosis Protein 2; RING Finger Protein 48; Apoptosis Inhibitor 1; IAP Homolog B; Hiap-2; C-IAP1;
Function
Multi-functional protein which regulates not only caspases and apoptosis, but also modulates inflammatory signaling and immunity, mitogenic kinase signaling, and cell proliferation, as well as cell invasion and metastasis. Acts as an E3 ubiquitin-protein ligase regulating NF-kappa-B signaling and regulates both canonical and non-canonical NF-kappa-B signaling by acting in opposite directions: acts as a positive regulator of the canonical pathway and suppresses constitutive activation of non-canonical NF-kappa-B signaling. The target proteins for its E3 ubiquitin-protein ligase activity include: RIPK1, RIPK2, RIPK3, RIPK4, CASP3, CASP7, CASP8, TRAF2, DIABLO/SMAC, MAP3K14/NIK, MAP3K5/ASK1, IKBKG/NEMO, IKBKE and MXD1/MAD1. Can also function as an E3 ubiquitin-protein ligase of the NEDD8 conjugation pathway, targeting effector caspases for neddylation and inactivation. Acts as an important regulator of innate immune signaling via regulation of Toll-like receptors (TLRs), Nodlike receptors (NLRs) and RIG-I like receptors (RLRs), collectively referred to as pattern recognition receptors (PRRs). Protects cells from spontaneous formation of the ripoptosome, a large multi-protein complex that has the capability to kill cancer cells in a caspase-dependent and caspase-independent manner. Suppresses ripoptosome formation by ubiquitinating RIPK1 and CASP8. Can stimulate the transcriptional activity of E2F1. Plays a role in the modulation of the cell cycle.
Biological Process
Cell surface receptor signaling pathway Source: ProtInc
I-kappaB kinase/NF-kappaB signaling Source: Reactome
MyD88-independent toll-like receptor signaling pathway Source: Reactome
Necroptotic process Source: Ensembl
Negative regulation of apoptotic process Source: UniProtKB
Negative regulation of necroptotic process Source: GO_Central
Negative regulation of ripoptosome assembly involved in necroptotic process Source: Ensembl
NIK/NF-kappaB signaling Source: UniProtKB
Placenta development Source: Ensembl
Positive regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProtKB
Positive regulation of protein K48-linked ubiquitination Source: UniProtKB
Positive regulation of protein K63-linked ubiquitination Source: UniProtKB
Positive regulation of protein monoubiquitination Source: UniProtKB
Positive regulation of protein ubiquitination Source: GO_Central
Proteasome-mediated ubiquitin-dependent protein catabolic process Source: UniProtKB
Protein deubiquitination Source: Reactome
Protein polyubiquitination Source: UniProtKB
Regulation of apoptotic process Source: UniProtKB
Regulation of cell cycle Source: UniProtKB
Regulation of cell differentiation Source: UniProtKB
Regulation of cell population proliferation Source: UniProtKB
Regulation of cysteine-type endopeptidase activity Source: UniProtKB
Regulation of inflammatory response Source: UniProtKB
Regulation of innate immune response Source: UniProtKB
Regulation of necroptotic process Source: UniProtKB
Regulation of NIK/NF-kappaB signaling Source: Ensembl
Regulation of nucleotide-binding oligomerization domain containing signaling pathway Source: UniProtKB
Regulation of reactive oxygen species metabolic process Source: Ensembl
Regulation of RIG-I signaling pathway Source: UniProtKB
Regulation of toll-like receptor signaling pathway Source: UniProtKB
Regulation of tumor necrosis factor-mediated signaling pathway Source: Reactome
Response to cAMP Source: Ensembl
Response to ethanol Source: Ensembl
Response to hypoxia Source: Ensembl
TRIF-dependent toll-like receptor signaling pathway Source: Reactome
Tumor necrosis factor-mediated signaling pathway Source: Reactome
Cellular Location
Cytoplasm; Nucleus. Agents that induce either the extrinsic or intrinsic apoptotic pathways promote its redistribution from the nuclear compartment to the cytoplasmic compartment. Associated with the midbody in telophase cells, and found diffusely in the nucleus of interphase cells.
PTM
Auto-ubiquitinated and degraded by the proteasome in apoptotic cells.
Upon stimulation of death receptors, including TNFRSF10B, recruited to receptors and cleaved by caspases. Proteolytic fragments remain associated with the receptors. This cleavage presumably inactivates the protein.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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