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Mouse Anti-CASP4 Recombinant Antibody (CBXC-2210) (CBMAB-C0224-CQ)

This product is a mouse antibody that recognizes CASP4. The antibody CBXC-2210 can be used for immunoassay techniques such as: WB.
See all CASP4 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBXC-2210
Antibody Isotype
IgG1
Application
WB

Basic Information

Immunogen
Recombinant protein corresponding to amino acids 81-270 of human CASP4 (NP_150649) produced in E. coli
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Caspase 4
Introduction
This gene encodes a protein that is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain and a large and small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This caspase is able to cleave and activate its own precursor protein, as well as caspase 1 precursor. When overexpressed, this gene induces cell apoptosis. Alternative splicing results in transcript variants encoding distinct isoforms.
Entrez Gene ID
837
UniProt ID
P49662
Alternative Names
Caspase 4; Caspase 4, Apoptosis-Related Cysteine Peptidase; Caspase 4, Apoptosis-Related Cysteine Protease; ICE And Ced-3 Homolog 2; Protease TX; ICE(Rel)-II; CASP-4; ICH-2; Mih1; Apoptotic Cysteine Protease Mih1/TX;
Function
Inflammatory caspase that acts as an essential effector of NLRP3 inflammasome-dependent CASP1 activation and IL1B and IL18 secretion in response to non-canonical activators, such as UVB radiation, cholera enterotoxin subunit B and cytosolic LPS (PubMed:23516580, PubMed:24879791, PubMed:25119034, PubMed:22246630, PubMed:26174085, PubMed:26173988, PubMed:26508369, PubMed:25964352).
Thiol protease that cleaves a tetrapeptide after an Asp residue at position P1 (PubMed:7797510, PubMed:23516580).
Independently of NLRP3 inflammasome and CASP1, promotes pyroptosis, through GSDMD cleavage and activation, followed by IL1A, IL18 and HMGB1 release in response to non-canonical inflammasome activators (PubMed:26375003, PubMed:32109412).
Plays a crucial role in the restriction of Salmonella typhimurium replication in colonic epithelial cells during infection: in later stages of the infection, LPS from cytosolic Salmonella triggers CASP4 activation, which catalyzes cleavage of GSDMD, resulting in pyroptosis of infected cells and their extrusion into the gut lumen, as well as in IL18 secretion (PubMed:25121752, PubMed:26375003, PubMed:25964352, PubMed:32109412).
Cleavage of GSDMD is not strictly dependent on the consensus cleavage site but depends on an exosite interface on CASP4 that recognizes and binds the Gasdermin-D, C-terminal (GSDMD-CT) part (PubMed:32109412).
Pyroptosis limits bacterial replication, while cytokine secretion promotes the recruitment and activation of immune cells and triggers mucosal inflammation (PubMed:25121752, PubMed:26375003, PubMed:25964352).
Involved in LPS-induced IL6 secretion; this activity may not require caspase enzymatic activity (PubMed:26508369).
Involved in cell death induced by endoplasmic reticulum stress and by treatment with cytotoxic APP peptides found Alzheimer's patient brains (PubMed:15123740, PubMed:22246630, PubMed:23661706).
Activated by direct binding to LPS without the need of an upstream sensor (PubMed:25119034).
Does not directly process IL1B (PubMed:7743998, PubMed:7797510, PubMed:7797592).
During non-canonical inflammasome activation, cuts CGAS and may play a role in the regulation of antiviral innate immune activation (PubMed:28314590).
Biological Process
Apoptotic process Source: ProtInc
Cellular response to amyloid-beta Source: ParkinsonsUK-UCL
Execution phase of apoptosis Source: GOC
Inflammatory response Source: UniProtKB-KW
Innate immune response Source: UniProtKB-KW
Intrinsic apoptotic signaling pathway Source: ParkinsonsUK-UCL
Intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress Source: ParkinsonsUK-UCL
Positive regulation of tumor necrosis factor-mediated signaling pathway Source: UniProtKB
Protein autoprocessing Source: UniProtKB
Proteolysis Source: ProtInc
Pyroptosis Source: UniProtKB
Regulation of apoptotic process Source: InterPro
Regulation of inflammatory response Source: UniProtKB
Cellular Location
Mitochondrion; Endoplasmic reticulum membrane; Secreted; Cytosol; Inflammasome. Predominantly localizes to the endoplasmic reticulum (ER). Association with the ER membrane requires TMEM214 (PubMed:15123740). Released in the extracellular milieu by keratinocytes following UVB irradiation (PubMed:22246630).
PTM
In response to activation signals, including endoplasmic reticulum stress or treatment with amyloid-beta A4 protein fragments (such as amyloid-beta protein 40), undergoes autoproteolytic cleavage.

Meng, L., Tian, Z., Long, X., Diao, T., Hu, M., Wang, M., ... & He, Y. (2021). Caspase 4 Overexpression as a Prognostic Marker in Clear Cell Renal Cell Carcinoma: A Study Based on the Cancer Genome Atlas Data Mining. Frontiers in genetics, 11, 1758.

Tian, X. X., Li, R., Liu, C., Liu, F., Yang, L. J., Wang, S. P., & Wang, C. L. (2021). NLRP6‐caspase 4 inflammasome activation in response to cariogenic bacterial lipoteichoic acid in human dental pulp inflammation. International Endodontic Journal, 54(6), 916-925.

Eltobgy, M., Zani, A., Kenney, A., Estfanous, S., Kim, E., Badr, A., ... & Amer, A. (2021). Caspase-4/11 exacerbates disease severity in SARS-CoV-2 infection by promoting inflammation and thrombosis. bioRxiv.

Jiao, Y., Wang, L., Lu, L., Liu, J., Li, X., Zhao, H., ... & Zheng, B. (2020). The role of caspase-4 and NLRP1 in MCF7 cell pyroptosis induced by hUCMSC-secreted factors. Stem cells international, 2020.

Terlizzi, M., Colarusso, C., De Rosa, I., Somma, P., Curcio, C., Aquino, R. P., ... & Sorrentino, R. (2020). Identification of a novel subpopulation of Caspase-4 positive non-small cell lung Cancer patients. Journal of Experimental & Clinical Cancer Research, 39(1), 1-17.

Terlizzi, M., Molino, A., Colarusso, C., Somma, P., De Rosa, I., Troisi, J., ... & Sorrentino, R. (2020). Altered lung tissue lipidomic profile in caspase-4 positive non-small cell lung cancer (NSCLC) patients. Oncotarget, 11(38), 3515.

Benaoudia, S., Martin, A., Puig Gamez, M., Gay, G., Lagrange, B., Cornut, M., ... & Henry, T. (2019). A genome‐wide screen identifies IRF2 as a key regulator of caspase‐4 in human cells. EMBO reports, 20(9), e48235.

Krause, K., Caution, K., Badr, A., Hamilton, K., Saleh, A., Patel, K., ... & Amer, A. O. (2018). CASP4/caspase-11 promotes autophagosome formation in response to bacterial infection. Autophagy, 14(11), 1928-1942.

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For research use only. Not intended for any clinical use.

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