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Mouse Anti-CBR3 Recombinant Antibody (CBFYC-0892) (CBMAB-C0947-FY)

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Published Data
CBMAB-C0947-FY-1
Mouse Anti-CBR3 Recombinant Antibody (CBFYC-0892) (CBMAB-C0947-FY) in IP
PSMB4-WT-HA or PSMB-des-HA and CBR3 were overexpressed in HEK293T cell. Lysates were subjected to IP using either anti-HA agarose as a control. ...View More
Deshmukh, F. K., Ben-Nissan, G., Olshina, M. A., Füzesi-Levi, M. G., Polkinghorn, C., Arkind, G., ... & Sharon, M. (2023). Allosteric regulation of the 20S proteasome by the Catalytic Core Regulators (CCRs) family. Nature communications, 14(1), 3126.
CBMAB-C0947-FY-2
Mouse Anti-CBR3 Recombinant Antibody (CBFYC-0892) (CBMAB-C0947-FY) in WB
Escherichia coli cell lysates overexpressing FLAG-tagged human β-subunits PSMB1 to PSMB7 were incubated with His6-CBR3 bound to Ni-beads for pull-down experiments. Bound subunits were detected using an anti-FLAG antibody. ...View More
Deshmukh, F. K., Ben-Nissan, G., Olshina, M. A., Füzesi-Levi, M. G., Polkinghorn, C., Arkind, G., ... & Sharon, M. (2023). Allosteric regulation of the 20S proteasome by the Catalytic Core Regulators (CCRs) family. Nature communications, 14(1), 3126.
Datasheet
SDS
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Basic Information

Host Animal
Mouse
Clone
CBFYC-0892
Application
WB, IP, IF, ELISA
Immunogen
Amino acids 126-176 mapping within an internal region of Carbonyl reductase 3 of human origin.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA1:100-1:1,000
IF(ICC)1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.
More Infomation

Target

Full Name
Carbonyl Reductase 3
Introduction
Carbonyl reductase 3 catalyzes the reduction of a large number of biologically and pharmacologically active carbonyl compounds to their corresponding alcohols. The enzyme is classified as a monomeric NADPH-dependent oxidoreductase. CBR3 contains three exons spanning 11.2 kilobases and is closely linked to another carbonyl reductase gene-CBR1.
Entrez Gene ID
Human874
Mouse109857
Rat304078
Dog487748
UniProt ID
HumanO75828
MouseQ8K354
RatB2GV72
DogL7N0B9
Alternative Names
Carbonyl Reductase 3; Short Chain Dehydrogenase/Reductase Family 21C Member 2; NADPH-Dependent Carbonyl Reductase 3; EC 1.1.1.184; Short Chain Dehydrogenase/Reductase Family 21C, Member 2; Epididymis Secretory Protein Li 25; Carbonyl Reductase (NADPH) 3
Function
Has low NADPH-dependent oxidoreductase activity towards 4-benzoylpyridine and menadione (in vitro).
Biological Process
Cognition Source: UniProtKB
Phylloquinone catabolic process Source: Ensembl
Xenobiotic metabolic process Source: Reactome
Cellular Location
Cytoplasm
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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Antibody Pairs

CBR3 Matched Antibody Pair (191) (CAT#: APMAB-191LY)

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CBR3 Matched Antibody Pair (191) (CAT#: APMAB-191LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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