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Mouse Anti- CD40 Recombinant Antibody (G28.5) (CBMAB-0925-LY)

This product is mouse antibody recognizes human CD4. The antibody OKT-5 immunoassay techniques such as: Function Assay.
See all CD40 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
G28.5
Antibody Isotype
IgG1
Application
FuncS

Basic Information

Specificity
Human
Antibody Isotype
IgG1
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
CD40 Molecule
Introduction
This gene is a member of the TNF-receptor superfamily. The encoded protein is a receptor on antigen-presenting cells of the immune system and is essential for mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Receptor for TNFSF5/CD40LG. Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion.
Entrez Gene ID
958
UniProt ID
P25942
Alternative Names
p50; Bp50; CDW40; TNFRSF5
Function
Cytokine that acts as a ligand to CD40/TNFRSF5 (PubMed:1280226, PubMed:31331973).
Costimulates T-cell proliferation and cytokine production (PubMed:8617933).
Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation (PubMed:8617933).
Induces the activation of NF-kappa-B (PubMed:15067037, PubMed:31331973).
Induces the activation of kinases MAPK8 and PAK2 in T-cells (PubMed:15067037).
Induces tyrosine phosphorylation of isoform 3 of CD28 (PubMed:15067037).
Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4 (By similarity).
Involved in immunoglobulin class switching (By similarity).
CD40 ligand, soluble form: Acts as a ligand for integrins, specifically ITGA5:ITGB1 and ITGAV:ITGB3; both integrins and the CD40 receptor are required for activation of CD40-CD40LG signaling, which have cell-type dependent effects, such as B-cell activation, NF-kappa-B signaling and anti-apoptotic signaling.
Biological Process
Activation of JUN kinase activity Source: UniProtKB
B cell differentiation Source: Ensembl
B cell proliferation Source: UniProtKB
CD40 signaling pathway Source: UniProtKB
Inflammatory response Source: UniProtKB
Integrin-mediated signaling pathway Source: UniProtKB
Isotype switching Source: UniProtKB
Leukocyte cell-cell adhesion Source: UniProtKB
Negative regulation of apoptotic process Source: UniProtKB
Platelet activation Source: UniProtKB
Positive regulation of endothelial cell apoptotic process Source: BHF-UCL
Positive regulation of interleukin-10 production Source: UniProtKB
Positive regulation of interleukin-12 production Source: UniProtKB
Positive regulation of interleukin-4 production Source: UniProtKB
Positive regulation of NF-kappaB transcription factor activity Source: UniProtKB
Positive regulation of T cell proliferation Source: UniProtKB
Regulation of immune response Source: Reactome
Regulation of immunoglobulin production Source: Ensembl
T cell costimulation Source: UniProtKB
Tumor necrosis factor-mediated signaling pathway Source: Reactome
Cellular Location
Cell membrane; Cell surface
CD40 ligand, soluble form: Secreted. Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and a matrix metalloproteinases (MMP) inhibitor-sensitive pathway.
Involvement in disease
Immunodeficiency with hyper-IgM, type 1 (HIGM1): Immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes. Affected males present at an early age (usually within the first year of life) recurrent bacterial and opportunistic infections, including Pneumocystis carinii pneumonia and intractable diarrhea due to cryptosporidium infection. Despite substitution treatment with intravenous immunoglobulin, the overall prognosis is rather poor, with a death rate of about 10% before adolescence.
Topology
Cytoplasmic: 1-22
Helical: 23-46
Extracellular: 47-261
PTM
The soluble form derives from the membrane form by proteolytic processing.
N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40.
Not O-glycosylated.

Tang, T., Cheng, X., Truong, B., Sun, L., Yang, X., & Wang, H. (2021). Molecular basis and therapeutic implications of CD40/CD40L immune checkpoint. Pharmacology & Therapeutics, 219, 107709.

Bullock, T. N. (2021). CD40 stimulation as a molecular adjuvant for cancer vaccines and other immunotherapies. Cellular & Molecular Immunology, 1-9.

Dakal, T. C., Dhabhai, B., Agarwal, D., Gupta, R., Nagda, G., Meena, A. R., ... & Sharma, A. (2020). Mechanistic basis of co-stimulatory CD40-CD40L ligation mediated regulation of immune responses in cancer and autoimmune disorders. Immunobiology, 225(2), 151899.

Richards, D. M., Sefrin, J. P., Gieffers, C., Hill, O., & Merz, C. (2020). Concepts for agonistic targeting of CD40 in immuno-oncology. Human vaccines & immunotherapeutics, 16(2), 377-387.

Lai, J. H., Luo, S. F., & Ho, L. J. (2019). Targeting the CD40-CD154 signaling pathway for treatment of autoimmune arthritis. Cells, 8(8), 927.

Schlievert, P. M., Cahill, M. P., Hostager, B. S., Brosnahan, A. J., Klingelhutz, A. J., Gourronc, F. A., ... & Leung, D. Y. (2019). Staphylococcal superantigens stimulate epithelial cells through CD40 to produce chemokines. MBio, 10(2), e00214-19.

Foster, A. E., Mahendravada, A., Shinners, N. P., Chang, W. C., Crisostomo, J., Lu, A., ... & Spencer, D. M. (2017). Regulated expansion and survival of chimeric antigen receptor-modified T cells using small molecule-dependent inducible MyD88/CD40. Molecular Therapy, 25(9), 2176-2188.

Chen, J., Song, Y., Bojadzic, D., Tamayo-Garcia, A., Landin, A. M., Blomberg, B. B., & Buchwald, P. (2017). Small-molecule inhibitors of the CD40–CD40L costimulatory protein–protein interaction. Journal of medicinal chemistry, 60(21), 8906-8922.

Aarts, S. A., Seijkens, T. T., Kusters, P. J., van der Pol, S. M., Zarzycka, B., Heijnen, P. D., ... & Lutgens, E. (2017). Inhibition of CD40-TRAF6 interactions by the small molecule inhibitor 6877002 reduces neuroinflammation. Journal of neuroinflammation, 14(1), 1-14.

Chen, J., Li, J. H., Zhao, S. J., Wang, D. Y., Zhang, W. Z., & Liang, W. J. (2017). Clinical significance of costimulatory molecules CD40/CD40L and CD134/CD134L in coronary heart disease: a case-control study. Medicine, 96(32).

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For research use only. Not intended for any clinical use.

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