Mouse Anti-CR1 Recombinant Antibody (CBYY-C1332) (V2LY-1206-LY1030)

Name: Mouse Anti-CR1 Recombinant Antibody (CBYY-C1332)
SKU: V2LY-1206-LY1030
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-CR1 Recombinant Antibody (CBYY-C1332) (V2LY-1206-LY1030) in FC

106 PBMCs isolated from healthy donors were activated for 10 minutes with 5 μg/mL F(ab')2 fragment of anti-human IgG+M+A and treated or not with 40 μg/mL heat-aggregated C3. ...View More

Kremlitzka, M., Mácsik-Valent, B., Polgár, A., Kiss, E., Poór, G., & Erdei, A. (2016). Complement receptor type 1 suppresses human B cell functions in SLE patients. Journal of immunology research, 2016(1), 5758192.

Distributed under Open Access license CC BY 4.0, without modification.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBYY-C1332

Application

WB, IP, IF, ELISA, IHC-P, FC

Immunogen

Human monocytes.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

0.1% gelatin

Preservative

0.09% sodium azide

Concentration

0.2 mg/ml

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Complement C3b/C4b Receptor 1 (Knops Blood Group)

Entrez Gene ID

1378

UniProt ID

P17927

Function

Membrane immune adherence receptor that plays a critical role in the capture and clearance of complement-opsonized pathogens by erythrocytes and monocytes/macrophages (PubMed:2963069).

Mediates the binding by these cells of particles and immune complexes that have activated complement to eliminate them from the circulation (PubMed:2963069).

Acts also in the inhibition of spontaneous complement activation by impairing the formation and function of the alternative and classical pathway C3/C5 convertases, and by serving as a cofactor for the cleavage by factor I of C3b to iC3b, C3c and C3d,g, and of C4b to C4c and C4d (PubMed:2972794, PubMed:8175757).

Plays also a role in immune regulation by contributing, upon ligand binding, to the generation of regulatory T cells from activated helper T cells (PubMed:25742728).

(Microbial infection) Acts as a receptor for Epstein-Barr virus.

Biological Process

ATP export Source: ARUK-UCL
Complement activation, alternative pathway Source: ARUK-UCL
Complement activation, classical pathway Source: UniProtKB-KW
Complement receptor mediated signaling pathway Source: ARUK-UCL
Immune complex clearance by erythrocytes Source: ARUK-UCL
Negative regulation of activation of membrane attack complex Source: ARUK-UCL
Negative regulation of complement activation Source: ARUK-UCL
Negative regulation of complement activation, alternative pathway Source: UniProtKB
Negative regulation of complement activation, classical pathway Source: UniProtKB
Negative regulation of cytolysis Source: ARUK-UCL
Negative regulation of immunoglobulin production Source: ARUK-UCL
Negative regulation of interferon-gamma production Source: ARUK-UCL
Negative regulation of interleukin-2 production Source: ARUK-UCL
Negative regulation of plasma cell differentiation Source: ARUK-UCL
Negative regulation of serine-type endopeptidase activity Source: UniProtKB
Negative regulation of T cell proliferation Source: ARUK-UCL
Neutrophil degranulation Source: Reactome
Plasma membrane organization Source: ARUK-UCL
Positive regulation of activation of membrane attack complex Source: ARUK-UCL
Positive regulation of cell population proliferation Source: ARUK-UCL
Positive regulation of regulatory T cell differentiation Source: ARUK-UCL
Positive regulation of serine-type endopeptidase activity Source: UniProtKB
Regulation of complement activation Source: Reactome
Regulation of regulatory T cell differentiation Source: Reactome

Cellular Location

Membrane

Topology

Extracellular: 42-1971
Helical: 1972-1996
Cytoplasmic: 1997-2039

Kisserli, A., Schneider, N., Audonnet, S., Tabary, T., Goury, A., Cousson, J., ... & Cohen, J. H. (2021). Acquired decrease of the C3b/C4b receptor (CR1, CD35) and increased C4d deposits on erythrocytes from ICU COVID-19 patients. Immunobiology, 226(3), 152093.

Wymann, S., Dai, Y., Nair, A. G., Cao, H., Powers, G. A., Schnell, A., ... & Hardy, M. P. (2021). A novel soluble complement receptor 1 fragment with enhanced therapeutic potential. Journal of Biological Chemistry, 296.

Qidwai, T. (2021). Human Complement Receptor 1 Polymorphisms. In Exploration of Host Genetic Factors associated with Malaria (pp. 113-122). Springer, Singapore.

Simmons, K. T., Mazzilli, J. L., Mueller-Ortiz, S. L., Domozhirov, A. Y., Garcia, C. A., Zsigmond, E. M., & Wetsel, R. A. (2020). Complement Receptor 1 (CR1/CD35)-expressing retinal pigment epithelial cells as a potential therapy for age-related macular degeneration. Molecular Immunology, 118, 91-98.

Kisserli, A., Audonnet, S., Duret, V., Tabary, T., Cohen, J. H. M., & Mahmoudi, R. (2020). Measuring erythrocyte complement receptor 1 using flow cytometry. JoVE (Journal of Visualized Experiments), (159), e60810.

Kucukkilic, E., Brookes, K., Barber, I., Guetta-Baranes, T., Morgan, K., & Hollox, E. J. (2018). Complement receptor 1 gene (CR1) intragenic duplication and risk of Alzheimer’s disease. Human genetics, 137(4), 305-314.

Kretzschmar, G. C., Oliveira, L. C., Nisihara, R. M., Velavan, T. P., Stinghen, S. T., Stahlke, E. R., ... & Boldt, A. B. W. (2018). Complement receptor 1 (CR1, CD35) association with susceptibility to leprosy. PLoS neglected tropical diseases, 12(8), e0006705.

Mahmoudi, R., Feldman, S., Kisserli, A., Duret, V., Tabary, T., Bertholon, L. A., ... & Cohen, J. H. M. (2018). Inherited and acquired decrease in complement receptor 1 (CR1) density on red blood cells associated with high levels of soluble CR1 in Alzheimer’s disease. International Journal of Molecular Sciences, 19(8), 2175.

Jacquet, M., Cioci, G., Fouet, G., Bally, I., Thielens, N. M., Gaboriaud, C., & Rossi, V. (2018). C1q and mannose-binding lectin interact with CR1 in the same region on CCP24-25 modules. Frontiers in Immunology, 9, 453.

Kisserli, A., Tabary, T., Cohen, J. H. M., Duret, V., & Mahmoudi, R. (2017). High-resolution melting PCR for complement receptor 1 length polymorphism genotyping: an innovative tool for Alzheimer's disease gene susceptibility assessment. JoVE (Journal of Visualized Experiments), (125), e56012.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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For research use only. Not intended for any clinical use.

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