Rabbit Anti-CYP17A1 Recombinant Antibody (
5A9) (V2LY-0725-LY1236)

Name: Rabbit Anti-CYP17A1 Recombinant Antibody (5A9)
SKU: V2LY-0725-LY1236
Rating: 5 (1 reviews)

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Tested Data

Rabbit Anti-CYP17A1 Recombinant Antibody (5A9) (V2LY-0725-LY1236) in WB

Western Blot
Positive WB detected in: HepG2 whole cell lysate, Rat Heart whole cell lysate, Rat Brain whole cell lysate
All lanes: CYP17A1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 58 kDa
Observed band size: 58 kDa

Rabbit Anti-CYP17A1 Recombinant Antibody (5A9) (V2LY-0725-LY1236) in IHC

IHC image of V2LY-0725-LY1236 diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Rabbit Anti-CYP17A1 Recombinant Antibody (5A9) (V2LY-0725-LY1236) in IF

Immunofluorescence staining of Hela Cells with V2LY-0725-LY1236 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

5A9

Application

ELISA, WB, IHC, IF

Immunogen

A synthesized peptide derived from human Cytochrome P450 17A1.

Host Species

Rabbit

Specificity

Human, Rat

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:5,000
IHC	1:50-1:200
IF	1:20-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Cytochrome P450 Family 17 Subfamily A Member 1

Entrez Gene ID

1586

UniProt ID

P05093

Function

A cytochrome P450 monooxygenase involved in corticoid and androgen biosynthesis (PubMed:9452426, PubMed:27339894, PubMed:22266943, PubMed:25301938).

Catalyzes 17-alpha hydroxylation of C21 steroids, which is common for both pathways. A second oxidative step, required only for androgen synthesis, involves an acyl-carbon cleavage. The 17-alpha hydroxy intermediates, as part of adrenal glucocorticoids biosynthesis pathway, are precursors of cortisol (PubMed:9452426, PubMed:25301938) (Probable).

Hydroxylates steroid hormones, pregnenolone and progesterone to form 17-alpha hydroxy metabolites, followed by the cleavage of the C17-C20 bond to form C19 steroids, dehydroepiandrosterone (DHEA) and androstenedione (PubMed:9452426, PubMed:27339894, PubMed:22266943, PubMed:25301938).

Has 16-alpha hydroxylase activity. Catalyzes 16-alpha hydroxylation of 17-alpha hydroxy pregnenolone, followed by the cleavage of the C17-C20 bond to form 16-alpha-hydroxy DHEA. Also 16-alpha hydroxylates androgens, relevant for estriol synthesis (PubMed:27339894, PubMed:25301938).

Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase) (PubMed:9452426, PubMed:27339894, PubMed:22266943, PubMed:25301938).

Biological Process

Androgen biosynthetic process Source: Reactome
Glucocorticoid biosynthetic process Source: Reactome
Hormone biosynthetic process Source: UniProtKB
Progesterone metabolic process Source: UniProtKB
Sex differentiation Source: ProtInc
Steroid biosynthetic process Source: ProtInc
Steroid metabolic process Source: UniProtKB

Cellular Location

Endoplasmic reticulum membrane; Microsome membrane

Involvement in disease

Adrenal hyperplasia 5 (AH5):
A form of congenital adrenal hyperplasia, a common recessive disease due to defective synthesis of cortisol. Congenital adrenal hyperplasia is characterized by androgen excess leading to ambiguous genitalia in affected females, rapid somatic growth during childhood in both sexes with premature closure of the epiphyses and short adult stature. Four clinical types: 'salt wasting' (SW, the most severe type), 'simple virilizing' (SV, less severely affected patients), with normal aldosterone biosynthesis, 'non-classic form' or late-onset (NC or LOAH) and 'cryptic' (asymptomatic).

PTM

Phosphorylation is necessary for 17,20-lyase, but not for 17-alpha-hydroxylase activity.

Zhai, G., Shu, T., Chen, K., Lou, Q., Jia, J., Huang, J., ... & Yin, Z. (2022). Successful production of an all-female common carp (Cyprinus carpio L.) population using cyp17a1-deficient neomale carp. Engineering, 8, 181-189.

Liu, Y., Denisov, I. G., Sligar, S. G., & Kincaid, J. R. (2021). Substrate-Specific Allosteric Effects on the Enhancement of CYP17A1 Lyase Efficiency by Cytochrome b 5. Journal of the American Chemical Society, 143(10), 3729-3733.

Tian, J., Li, Y., Fu, H., Ren, L., He, Y., Zhai, S., ... & Liu, S. (2021). Physiological role of CYP17A1-like in cadmium detoxification and its transcriptional regulation in the Pacific oyster, Crassostrea gigas. Science of The Total Environment, 796, 149039.

Xiao, F., Song, X., Tian, P., Gan, M., Verkhivker, G. M., & Hu, G. (2020). Comparative dynamics and functional mechanisms of the CYP17A1 tunnels regulated by ligand binding. Journal of Chemical Information and Modeling, 60(7), 3632-3647.

Lin, H. Y., Ko, C. Y., Kao, T. J., Yang, W. B., Tsai, Y. T., Chuang, J. Y., ... & Hsu, T. I. (2019). CYP17A1 Maintains the survival of glioblastomas by regulating SAR1-mediated endoplasmic reticulum health and redox homeostasis. Cancers, 11(9), 1378.

Kaur, R., Kaur, T., & Kaur, A. (2018). Genetic association study from North India to analyze association of CYP19A1 and CYP17A1 with polycystic ovary syndrome. Journal of assisted reproduction and genetics, 35(6), 1123-1129.

Almassi, N., Reichard, C., Li, J., Russell, C., Perry, J., Ryan, C. J., ... & Sharifi, N. (2018). HSD3B1 and response to a nonsteroidal CYP17A1 inhibitor in castration-resistant prostate cancer. JAMA oncology, 4(4), 554-557.

Zhai, G., Shu, T., Xia, Y., Lu, Y., Shang, G., Jin, X., ... & Yin, Z. (2018). Characterization of sexual trait development in cyp17a1-deficient zebrafish. Endocrinology, 159(10), 3549-3562.

Li, A., Yadav, R., White, J. K., Herroon, M. K., Callahan, B. P., Podgorski, I., ... & Kodanko, J. J. (2017). Illuminating cytochrome P450 binding: Ru (ii)-caged inhibitors of CYP17A1. Chemical Communications, 53(26), 3673-3676.

Sivonova, M. K., Jurecekova, J., Tatarkova, Z., Kaplan, P., Lichardusova, L., & Hatok, J. (2017). The role of CYP17A1 in prostate cancer development: structure, function, mechanism of action, genetic variations and its inhibition. Gen Physiol Biophys, 36(5), 487-499.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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