Mouse Anti-CYP4A11 Recombinant Antibody (CBWJC-2109) (V2LY-0125-LY1003)

Name: Mouse Anti-CYP4A11 Recombinant Antibody (CBWJC-2109)
SKU: V2LY-0125-LY1003
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBWJC-2109

Application

WB, ELISA, IP

Immunogen

Amino acids 499-507 of human CYP4A11.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:100-1:1,000
IP	1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium Azide

Concentration

0.2 mg/mL

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Cytochrome P450 Family 4 Subfamily A Member 11

Entrez Gene ID

Human	1579
Rat	24306

UniProt ID

Human	Q02928
Rat	P20816

Function

A cytochrome P450 monooxygenase involved in the metabolism of fatty acids and their oxygenated derivatives (oxylipins) (PubMed:7679927, PubMed:1739747, PubMed:8914854, PubMed:10553002, PubMed:10660572, PubMed:15611369).

Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase) (PubMed:7679927, PubMed:1739747, PubMed:8914854, PubMed:10553002, PubMed:10660572, PubMed:15611369).

Catalyzes predominantly the oxidation of the terminal carbon (omega-oxidation) of saturated and unsaturated fatty acids, the catalytic efficiency decreasing in the following order: dodecanoic> tetradecanoic> (9Z)-octadecenoic> (9Z,12Z)-octadecadienoic> hexadecanoic acid (PubMed:10553002, PubMed:10660572).

Acts as a major omega-hydroxylase for dodecanoic (lauric) acid in liver (PubMed:7679927, PubMed:1739747, PubMed:8914854, PubMed:15611369).

Participates in omega-hydroxylation of (5Z,8Z,11Z,14Z)-eicosatetraenoic acid (arachidonate) to 20-hydroxyeicosatetraenoic acid (20-HETE), a signaling molecule acting both as vasoconstrictive and natriuretic with overall effect on arterial blood pressure (PubMed:10620324, PubMed:10660572, PubMed:15611369).

Can also catalyze the oxidation of the penultimate carbon (omega-1 oxidation) of fatty acids with lower efficiency (PubMed:7679927).

May contribute to the degradation of saturated very long-chain fatty acids (VLCFAs) such as docosanoic acid, by catalyzing successive omega-oxidations to the corresponding dicarboxylic acid, thereby initiating chain shortening (PubMed:18182499).

Omega-hydroxylates (9R,10S)-epoxy-octadecanoate stereoisomer (PubMed:15145985).

Plays a minor role in omega-oxidation of long-chain 3-hydroxy fatty acids (PubMed:18065749).

Has little activity toward prostaglandins A1 and E1 (PubMed:7679927).

Biological Process

Arachidonic acid metabolic process Source: UniProtKB
Epoxygenase P450 pathway Source: UniProtKB
Fatty acid metabolic process Source: Reactome
Icosanoid biosynthetic process Source: GO_Central
Kidney development Source: GO_Central
Lauric acid metabolic process Source: GO_Central
Leukotriene metabolic process Source: UniProtKB
Linoleic acid metabolic process Source: GO_Central
Long-chain fatty acid metabolic process Source: BHF-UCL
Omega-hydroxylase P450 pathway Source: Reactome
Oxylipin biosynthetic process Source: UniProtKB-UniPathway
Positive regulation of icosanoid secretion Source: UniProtKB
Pressure natriuresis Source: UniProtKB
Regulation of metabolic process Source: Reactome
Renal water homeostasis Source: UniProtKB
Sodium ion homeostasis Source: UniProtKB

Cellular Location

Endoplasmic reticulum membrane; Microsome membrane

Gao, H., Cao, Y., Xia, H., Zhu, X., & Jin, Y. (2020). CYP4A11 is involved in the development of nonalcoholic fatty liver disease via ROS‑induced lipid peroxidation and inflammation. International journal of molecular medicine, 45(4), 1121-1129.

Han, L., Zhang, H., Zeng, Y., Lv, Y., Tao, L., Ma, J., ... & Chen, L. (2020). Identification of the miRNA-3185/CYP4A11 axis in cardiac tissue as a biomarker for mechanical asphyxia. Forensic Science International, 311, 110293.

Kim, S., Kim, J. M., Lee, H. J., Lim, J. S., Seong, I. O., & Kim, K. H. (2020). Alteration of CYP4A11 expression in renal cell carcinoma: diagnostic and prognostic implications. Journal of Cancer, 11(6), 1478.

Borin, T. F., Ali, S., Angara, K., Rashid, M., Myers, S., Chawla, D., ... & Arbab, A. S. (2019). CYP4A11 overexpression increases aggressiveness in glioblastoma and breast cancer.

Yu, K., Zhang, T., & Li, X. (2018). Genetic role of CYP4A11 polymorphisms in the risk of developing cardiovascular and cerebrovascular diseases. Annals of Human Genetics, 82(6), 370-381.

Sirotina, S., Ponomarenko, I., Kharchenko, A., Bykanova, M., Bocharova, A., Vagaytseva, K., ... & Polonikov, A. (2018). A novel polymorphism in the promoter of the CYP4A11 gene is associated with susceptibility to coronary artery disease. Disease Markers, 2018.

Yamaori, S., Araki, N., Shionoiri, M., Ikehata, K., Kamijo, S., Ohmori, S., & Watanabe, K. (2018). A specific probe substrate for evaluation of CYP4A11 activity in human tissue microsomes and a highly Selective CYP4A11 inhibitor: luciferin-4A and epalrestat. Journal of Pharmacology and Experimental Therapeutics, 366(3), 446-457.

Yang, M., Lv, J., Zhang, L., Li, M., Zhou, Y., Lan, X., ... & Chen, H. (2017). Association study and expression analysis of CYP4A11 gene copy number variation in Chinese cattle. Scientific reports, 7(1), 1-8.

Zhang, H., Jin, L., Mu, T., Fan, Y., Zhang, H., Zhu, Y., ... & Tang, S. (2017). Associations of CYP4A11 gene–gene and gene–smoking interactions with essential hypertension in the male eastern Chinese Han population. Clinical and Experimental Hypertension, 39(5), 448-453.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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