Mouse Anti-D2HGDH Recombinant Antibody (D0134) (V2LY-0125-LY1040)

Name: Mouse Anti-D2HGDH Recombinant Antibody (D0134)
SKU: V2LY-0125-LY1040
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

D0134

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 41-146 mapping near the N-terminus of human D2HGDH.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:100-1:1,000
IP	1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)
ELISA	1:100-1:1,000
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium Azide

Concentration

0.2 mg/mL

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

D-2-Hydroxyglutarate Dehydrogenase

Entrez Gene ID

728294

UniProt ID

Q8N465

Function

Catalyzes the oxidation of D-2-hydroxyglutarate to alpha-ketoglutarate.

Biological Process

2-oxoglutarate metabolic process Source: Reactome
Cellular protein metabolic process Source: HGNC-UCL
Response to cobalt ion Source: HGNC-UCL
Response to manganese ion Source: HGNC-UCL
Response to zinc ion Source: HGNC-UCL

Cellular Location

Mitochondrion

Involvement in disease

D-2-hydroxyglutaric aciduria 1 (D2HGA1):
A rare recessive neurometabolic disorder causing developmental delay, epilepsy, hypotonia, and dysmorphic features. Both a mild and a severe phenotype exist. The severe phenotype is homogeneous and is characterized by early infantile-onset epileptic encephalopathy and cardiomyopathy. The mild phenotype has a more variable clinical presentation. Diagnosis is based on the presence of an excess of D-2-hydroxyglutaric acid in the urine.

Xiao, D., Zhang, W., Guo, X., Liu, Y., Hu, C., Guo, S., ... & Xu, P. (2021). A D-2-hydroxyglutarate biosensor based on specific transcriptional regulator DhdR. Nature communications, 12(1), 1-14.

Chou, F. J., Liu, Y., Lang, F., & Yang, C. (2021). D-2-Hydroxyglutarate in Glioma Biology. Cells, 10(9), 2345.

Berger, R. S., Ellmann, L., Reinders, J., Kreutz, M., Stempfl, T., Oefner, P. J., & Dettmer, K. (2019). Degradation of D-2-hydroxyglutarate in the presence of isocitrate dehydrogenase mutations. Scientific reports, 9(1), 1-11.

Pop, A., Struys, E. A., Jansen, E. E., Fernandez, M. R., Kanhai, W. A., van Dooren, S. J., ... & Salomons, G. S. (2019). D‐2‐hydroxyglutaric aciduria Type I: Functional analysis of D2HGDH missense variants. Human mutation, 40(7), 975-982.

Toplak, M., Brunner, J., Schmidt, J., & Macheroux, P. (2019). Biochemical characterization of human D-2-hydroxyglutarate dehydrogenase and two disease related variants reveals the molecular cause of D-2-hydroxyglutaric aciduria. Biochimica et Biophysica Acta (BBA)-Proteins and Proteomics, 1867(11), 140255.

Guo, X., Zhang, M., Cao, M., Zhang, W., Kang, Z., Xu, P., ... & Gao, C. (2018). d-2-Hydroxyglutarate dehydrogenase plays a dual role in l-serine biosynthesis and d-malate utilization in the bacterium Pseudomonas stutzeri. Journal of Biological Chemistry, 293(40), 15513-15523.

Han, J., Jackson, D., Holm, J., Turner, K., Ashcraft, P., Wang, X., ... & Theiss, A. L. (2018). Elevated d-2-hydroxyglutarate during colitis drives progression to colorectal cancer. Proceedings of the National Academy of Sciences, 115(5), 1057-1062.

Zhang, W., Zhang, M., Gao, C., Zhang, Y., Ge, Y., Guo, S., ... & Xu, P. (2017). Coupling between d-3-phosphoglycerate dehydrogenase and d-2-hydroxyglutarate dehydrogenase drives bacterial l-serine synthesis. Proceedings of the National Academy of Sciences, 114(36), E7574-E7582.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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