Mouse Anti-DDR2 Recombinant Antibody (0994) (CBMAB-0994-YC)

Name: Mouse Anti-DDR2 Recombinant Antibody (0994)
SKU: CBMAB-0994-YC
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-DDR2 Recombinant Antibody (0994) (CBMAB-0994-YC-1) in IF

Representative images of α-SMA expression in adult mouse CFs after the indicated treatments. Scale bar, 200μm. ...View More

Weng, H., Zou, W., Tian, F., Xie, H., Liu, A., Liu, W., ... & Shu, X. (2024). Inhalable cardiac targeting peptide modified nanomedicine prevents pressure overload heart failure in male mice. Nature Communications, 15(1), 6058.

Mouse Anti-DDR2 Recombinant Antibody (0994) (CBMAB-0994-YC-2) in IF

Representative immunofluorescent photomicrographs of TRPC1 or TRPC6 (green) localization.
Cardiomyocytes labeled with α-sarcomeric actin (α-SCA) (red), macrophages labeled with CD68 (red), or
fibrocytes labeled with DDR2 (red) in ventricles of WT, Trpc1-/-, and Trpc6-/- mice 6 h after LPS challenge.
Blue indicates cellular nuclei stained with DAPI. The colocalization is shown in yellow (white arrows) (n =
6 images from 3 male mice per group). ...View More

Tang, N., Tian, W., Ma, G. Y., Xiao, X., Zhou, L., Li, Z. Z., ... & Cao, W. (2022). TRPC channels blockade abolishes endotoxemic cardiac dysfunction by hampering intracellular inflammation and Ca2+ leakage. Nature Communications, 13(1), 7455.

Mouse Anti-DDR2 Recombinant Antibody (0994) (CBMAB-0994-YC-3) in IF

Immunofluorescence (IF) staining for phosphorylated DDR1, DDR2 and collagen1a in TGF-β1-induced PSCs. β-actin was used as loading control. All values are represented as mean±SEM (n=3). ...View More

Bansod, S., Saifi, M. A., & Godugu, C. (2021). Inhibition of discoidin domain receptors by imatinib prevented pancreatic fibrosis demonstrated in experimental chronic pancreatitis model. Scientific Reports, 11(1), 12894.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

0994

Application

WB, IP, IF, IHC-P

Immunogen

Amino acids 422-639 of human DDR2.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium Azide

Concentration

0.2 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Discoidin Domain Receptor Tyrosine Kinase 2

Introduction

Discoidin domain receptor tyrosine kinase 2 (DDR2) is a member of the discoidin domain receptor subclass of the receptor tyrosine kinase (RTKs) protein family. DDR2 is a collagen-induced receptor that activates signal transduction pathways involved in cell adhesion, proliferation, and extracellular matrix remodeling. Mutations in this gene are the cause of short limb-hand type spondylometaepiphyseal dysplasia.

Entrez Gene ID

Human	4921
Mouse	18214
Rat	685781

UniProt ID

Human	Q16832
Mouse	Q62371
Rat	B1WC09

Alternative Names

TKT; MIG20a; NTRKR3; TYRO10

Function

Tyrosine kinase involved in the regulation of tissues remodeling (PubMed:30449416).

It functions as cell surface receptor for fibrillar collagen and regulates cell differentiation, remodeling of the extracellular matrix, cell migration and cell proliferation. Required for normal bone development. Regulates osteoblast differentiation and chondrocyte maturation via a signaling pathway that involves MAP kinases and leads to the activation of the transcription factor RUNX2. Regulates remodeling of the extracellular matrix by up-regulation of the collagenases MMP1, MMP2 and MMP13, and thereby facilitates cell migration and tumor cell invasion. Promotes fibroblast migration and proliferation, and thereby contributes to cutaneous wound healing.

Biological Process

Biomineral tissue development Source: UniProtKB
Cell adhesion Source: ProtInc
Chondrocyte proliferation Source: UniProtKB
Collagen-activated tyrosine kinase receptor signaling pathway Source: UniProtKB
Collagen fibril organization Source: UniProtKB
Endochondral bone growth Source: UniProtKB
Extracellular matrix organization Source: Reactome
Multicellular organism development Source: GO_Central
Ossification Source: UniProtKB-KW
Peptidyl-tyrosine phosphorylation Source: UniProtKB
Positive regulation of DNA-binding transcription factor activity Source: UniProtKB
Positive regulation of extracellular matrix disassembly Source: UniProtKB
Positive regulation of fibroblast migration Source: UniProtKB
Positive regulation of fibroblast proliferation Source: UniProtKB
Positive regulation of kinase activity Source: GO_Central
Positive regulation of osteoblast differentiation Source: UniProtKB
Positive regulation of protein kinase activity Source: UniProtKB
Protein autophosphorylation Source: UniProtKB
Regulation of bone mineralization Source: UniProtKB
Regulation of extracellular matrix disassembly Source: UniProtKB
Regulation of tissue remodeling Source: UniProtKB
Signal transduction Source: ProtInc
Transmembrane receptor protein tyrosine kinase signaling pathway Source: GO_Central

Cellular Location

Cell membrane

Involvement in disease

Spondyloepimetaphyseal dysplasia, short limb-hand type (SEMD-SL):
A bone disease characterized by short-limbed dwarfism, a narrow chest with pectus excavatum, brachydactyly in the hands and feet, a characteristic craniofacial appearance and premature calcifications. The radiological findings are distinctive and comprise short long bones throughout the skeleton with striking epiphyses that are stippled, flattened and fragmented and flared, irregular metaphyses. Platyspondyly in the spine with wide intervertebral spaces is observed and some vertebral bodies are pear-shaped with central humps, anterior protrusions and posterior scalloping.
Warburg-Cinotti syndrome (WRCN):
An autosomal dominant disease characterized by progressive corneal neovascularization, keloid formation, chronic skin ulcers, wasting of subcutaneous tissue, flexion contractures of the fingers, and acro-osteolysis.

Topology

Extracellular: 22-399
Helical: 400-421
Cytoplasmic: 422-855

PTM

N-glycosylated.
Tyrosine phosphorylated in response to collagen binding. Phosphorylated by SRC; this is required for activation and subsequent autophosphorylation on additional tyrosine residues.

Berestjuk, I., Lecacheur, M., Carminati, A., Diazzi, S., Rovera, C., Prod’homme, V., ... & Tartare‐Deckert, S. (2022). Targeting Discoidin Domain Receptors DDR1 and DDR2 overcomes matrix‐mediated tumor cell adaptation and tolerance to BRAF‐targeted therapy in melanoma. EMBO molecular medicine, 14(2), e11814.

Lin, C. C., Yang, W. H., Lin, Y. T., Tang, X., Chen, P. H., Ding, C. K. C., ... & Chi, J. T. (2021). DDR2 upregulation confers ferroptosis susceptibility of recurrent breast tumors through the Hippo pathway. Oncogene, 40(11), 2018-2034.

Matada, G. S. P., Das, A., Dhiwar, P. S., & Ghara, A. (2021). DDR1 and DDR2: A review on signaling pathway and small molecule inhibitors as an anticancer agent. Medicinal Chemistry Research, 30(3), 535-551.

Barcus, C. E., Hwang, P. Y., Morikis, V., Brenot, A., Pence, P., Clarke, M., & Longmore, G. D. (2021). Tyrosine kinase-independent actions of DDR2 in tumor cells and cancer-associated fibroblasts influence tumor invasion, migration and metastasis. Journal of cell science, 134(19), jcs258431.

Deng, L., Liu, G., Zheng, C., Zhang, L., Kang, Y., & Yang, F. (2019). Circ-LAMP1 promotes T-cell lymphoblastic lymphoma progression via acting as a ceRNA for miR-615-5p to regulate DDR2 expression. Gene, 701, 146-151.

Tu, M. M., Lee, F. Y., Jones, R. T., Kimball, A. K., Saravia, E., Graziano, R. F., ... & Theodorescu, D. (2019). Targeting DDR2 enhances tumor response to anti–PD-1 immunotherapy. Science advances, 5(2), eaav2437.

Bayer, S. V., Grither, W. R., Brenot, A., Hwang, P. Y., Barcus, C. E., Ernst, M., ... & Longmore, G. D. (2019). DDR2 controls breast tumor stiffness and metastasis by regulating integrin mediated mechanotransduction in CAFs. Elife, 8, e45508.

Cario, M. (2018). DDR1 and DDR2 in skin. Cell Adhesion & Migration, 12(4), 386-393.

Grither, W. R., & Longmore, G. D. (2018). Inhibition of tumor–microenvironment interaction and tumor invasion by small-molecule allosteric inhibitor of DDR2 extracellular domain. Proceedings of the National Academy of Sciences, 115(33), E7786-E7794.

Gonzalez, M. E., Martin, E. E., Anwar, T., Arellano-Garcia, C., Medhora, N., Lama, A., ... & Kleer, C. G. (2017). Mesenchymal stem cell-induced DDR2 mediates stromal-breast cancer interactions and metastasis growth. Cell reports, 18(5), 1215-1228.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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For research use only. Not intended for any clinical use.

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